Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1740 and Pf6N2E2_1741 overlap by 4 nucleotides Pf6N2E2_1741 and Pf6N2E2_1742 are separated by 187 nucleotides
Pf6N2E2_1739 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 2,051,415 to 2,052,698
_1739
Pf6N2E2_1740 - Transketolase, C-terminal section (EC 2.2.1.1), at 2,052,688 to 2,053,698
_1740
Pf6N2E2_1741 - Transketolase, N-terminal section (EC 2.2.1.1), at 2,053,695 to 2,054,579
_1741
Pf6N2E2_1742 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 2,054,767 to 2,056,158
_1742
Position (kb)
2053
2054
2055 Strain fitness (log2 ratio)
-2
-1
0
1 at 2052.782 kb on + strand at 2052.782 kb on + strand at 2052.845 kb on - strand, within Pf6N2E2_1740 at 2052.845 kb on - strand, within Pf6N2E2_1740 at 2053.313 kb on + strand, within Pf6N2E2_1740 at 2053.313 kb on + strand, within Pf6N2E2_1740 at 2053.371 kb on - strand, within Pf6N2E2_1740 at 2053.629 kb on - strand at 2053.629 kb on - strand at 2053.665 kb on - strand at 2053.792 kb on + strand, within Pf6N2E2_1741 at 2053.793 kb on - strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.107 kb on + strand, within Pf6N2E2_1741 at 2054.118 kb on + strand, within Pf6N2E2_1741 at 2054.119 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.399 kb on - strand, within Pf6N2E2_1741 at 2054.401 kb on + strand, within Pf6N2E2_1741 at 2054.444 kb on - strand, within Pf6N2E2_1741 at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.899 kb on + strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.900 kb on - strand at 2054.926 kb on + strand, within Pf6N2E2_1742 at 2054.926 kb on + strand, within Pf6N2E2_1742 at 2054.927 kb on - strand, within Pf6N2E2_1742 at 2055.341 kb on - strand, within Pf6N2E2_1742
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,052,782 + +0.2 2,052,782 + +0.2 2,052,845 - Pf6N2E2_1740 0.16 +0.2 2,052,845 - Pf6N2E2_1740 0.16 +0.2 2,053,313 + Pf6N2E2_1740 0.62 -0.3 2,053,313 + Pf6N2E2_1740 0.62 -0.8 2,053,371 - Pf6N2E2_1740 0.68 -0.6 2,053,629 - +0.2 2,053,629 - +0.2 2,053,665 - +0.2 2,053,792 + Pf6N2E2_1741 0.11 +0.2 2,053,793 - Pf6N2E2_1741 0.11 +0.2 2,054,107 + Pf6N2E2_1741 0.47 -0.8 2,054,107 + Pf6N2E2_1741 0.47 -0.8 2,054,118 + Pf6N2E2_1741 0.48 -0.8 2,054,119 - Pf6N2E2_1741 0.48 -0.8 2,054,399 - Pf6N2E2_1741 0.80 +0.2 2,054,399 - Pf6N2E2_1741 0.80 -0.6 2,054,399 - Pf6N2E2_1741 0.80 -2.1 2,054,401 + Pf6N2E2_1741 0.80 -0.8 2,054,444 - Pf6N2E2_1741 0.85 -1.8 2,054,899 + -0.8 2,054,899 + +0.2 2,054,899 + -0.8 2,054,899 + +0.2 2,054,899 + +0.2 2,054,900 - -0.8 2,054,900 - +0.2 2,054,900 - -0.8 2,054,900 - +0.2 2,054,926 + Pf6N2E2_1742 0.11 +0.2 2,054,926 + Pf6N2E2_1742 0.11 +0.2 2,054,927 - Pf6N2E2_1742 0.11 +0.0 2,055,341 - Pf6N2E2_1742 0.41 -0.4
Or see this region's nucleotide sequence