Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1602 and Pf6N2E2_1603 are separated by 163 nucleotides Pf6N2E2_1603 and Pf6N2E2_1604 are separated by 62 nucleotides
Pf6N2E2_1602 - hypothetical protein, at 1,874,530 to 1,875,312
_1602
Pf6N2E2_1603 - hypothetical protein, at 1,875,476 to 1,875,904
_1603
Pf6N2E2_1604 - Acetyltransferase, at 1,875,967 to 1,876,467
_1604
Position (kb)
1875
1876 Strain fitness (log2 ratio)
-2
-1
0
1 at 1874.508 kb on - strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.831 kb on + strand, within Pf6N2E2_1602 at 1874.936 kb on + strand, within Pf6N2E2_1602 at 1875.005 kb on + strand, within Pf6N2E2_1602 at 1875.154 kb on - strand, within Pf6N2E2_1602 at 1875.402 kb on - strand at 1875.402 kb on - strand at 1875.402 kb on - strand at 1875.404 kb on - strand at 1875.418 kb on - strand at 1875.419 kb on + strand at 1875.444 kb on + strand at 1875.445 kb on - strand at 1875.499 kb on + strand at 1875.518 kb on - strand at 1875.521 kb on + strand, within Pf6N2E2_1603 at 1875.521 kb on + strand, within Pf6N2E2_1603 at 1875.522 kb on - strand, within Pf6N2E2_1603 at 1875.522 kb on - strand, within Pf6N2E2_1603 at 1875.626 kb on + strand, within Pf6N2E2_1603 at 1875.822 kb on - strand, within Pf6N2E2_1603 at 1875.929 kb on - strand at 1875.961 kb on - strand at 1875.969 kb on - strand at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.085 kb on + strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.086 kb on - strand, within Pf6N2E2_1604 at 1876.313 kb on + strand, within Pf6N2E2_1604 at 1876.349 kb on + strand, within Pf6N2E2_1604 at 1876.374 kb on + strand, within Pf6N2E2_1604 at 1876.500 kb on + strand at 1876.500 kb on + strand at 1876.819 kb on + strand at 1876.819 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 1,874,508 - -0.6 1,874,543 + +0.3 1,874,543 + -1.2 1,874,543 + +0.3 1,874,766 + Pf6N2E2_1602 0.30 +0.3 1,874,766 + Pf6N2E2_1602 0.30 +0.3 1,874,768 + Pf6N2E2_1602 0.30 +0.3 1,874,768 + Pf6N2E2_1602 0.30 -0.5 1,874,831 + Pf6N2E2_1602 0.38 +0.3 1,874,936 + Pf6N2E2_1602 0.52 -1.3 1,875,005 + Pf6N2E2_1602 0.61 +0.3 1,875,154 - Pf6N2E2_1602 0.80 +0.3 1,875,402 - +0.3 1,875,402 - -2.1 1,875,402 - +0.3 1,875,404 - -0.7 1,875,418 - +0.3 1,875,419 + -0.3 1,875,444 + +0.3 1,875,445 - +0.3 1,875,499 + -0.7 1,875,518 - -1.0 1,875,521 + Pf6N2E2_1603 0.10 -0.7 1,875,521 + Pf6N2E2_1603 0.10 -1.3 1,875,522 - Pf6N2E2_1603 0.11 +0.3 1,875,522 - Pf6N2E2_1603 0.11 -0.7 1,875,626 + Pf6N2E2_1603 0.35 +0.3 1,875,822 - Pf6N2E2_1603 0.81 -0.7 1,875,929 - +0.3 1,875,961 - -0.1 1,875,969 - +0.3 1,876,085 + Pf6N2E2_1604 0.24 +0.3 1,876,085 + Pf6N2E2_1604 0.24 -0.2 1,876,085 + Pf6N2E2_1604 0.24 +0.3 1,876,085 + Pf6N2E2_1604 0.24 -0.7 1,876,085 + Pf6N2E2_1604 0.24 +0.3 1,876,086 - Pf6N2E2_1604 0.24 +0.3 1,876,086 - Pf6N2E2_1604 0.24 -0.7 1,876,086 - Pf6N2E2_1604 0.24 +0.3 1,876,086 - Pf6N2E2_1604 0.24 -0.7 1,876,313 + Pf6N2E2_1604 0.69 -0.6 1,876,349 + Pf6N2E2_1604 0.76 -0.8 1,876,374 + Pf6N2E2_1604 0.81 +0.3 1,876,500 + +0.3 1,876,500 + -0.7 1,876,819 + -0.5 1,876,819 + -0.2
Or see this region's nucleotide sequence