Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1561

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1560 and Pf6N2E2_1561 are separated by 113 nucleotidesPf6N2E2_1561 and Pf6N2E2_1562 are separated by 154 nucleotides Pf6N2E2_1560 - Glycine cleavage system transcriptional activator GcvA, at 1,821,858 to 1,822,763 _1560 Pf6N2E2_1561 - Glutathione S-transferase (EC 2.5.1.18), at 1,822,877 to 1,823,476 _1561 Pf6N2E2_1562 - Threonine dehydratase (EC 4.3.1.19), at 1,823,631 to 1,824,587 _1562 Position (kb) 1822 1823 1824Strain fitness (log2 ratio) -2 -1 0 1 2at 1821.903 kb on - strandat 1821.903 kb on - strandat 1822.028 kb on - strand, within Pf6N2E2_1560at 1822.373 kb on + strand, within Pf6N2E2_1560at 1822.373 kb on + strand, within Pf6N2E2_1560at 1822.558 kb on + strand, within Pf6N2E2_1560at 1822.558 kb on + strand, within Pf6N2E2_1560at 1822.703 kb on + strandat 1822.730 kb on + strandat 1822.795 kb on - strandat 1822.799 kb on + strandat 1822.799 kb on + strandat 1822.886 kb on + strandat 1822.887 kb on - strandat 1822.887 kb on - strandat 1823.027 kb on + strand, within Pf6N2E2_1561at 1823.027 kb on + strand, within Pf6N2E2_1561at 1823.027 kb on + strand, within Pf6N2E2_1561at 1823.090 kb on + strand, within Pf6N2E2_1561at 1823.179 kb on + strand, within Pf6N2E2_1561at 1823.180 kb on - strand, within Pf6N2E2_1561at 1823.225 kb on - strand, within Pf6N2E2_1561at 1823.279 kb on + strand, within Pf6N2E2_1561at 1823.279 kb on + strand, within Pf6N2E2_1561at 1823.280 kb on - strand, within Pf6N2E2_1561at 1823.342 kb on + strand, within Pf6N2E2_1561at 1823.342 kb on + strand, within Pf6N2E2_1561at 1823.342 kb on + strand, within Pf6N2E2_1561at 1823.342 kb on + strand, within Pf6N2E2_1561at 1823.343 kb on - strand, within Pf6N2E2_1561at 1823.343 kb on - strand, within Pf6N2E2_1561at 1823.343 kb on - strand, within Pf6N2E2_1561at 1823.343 kb on - strand, within Pf6N2E2_1561at 1823.475 kb on - strandat 1823.650 kb on + strandat 1823.651 kb on - strandat 1823.651 kb on - strandat 1823.666 kb on + strandat 1823.782 kb on + strand, within Pf6N2E2_1562at 1823.782 kb on + strand, within Pf6N2E2_1562at 1823.782 kb on + strand, within Pf6N2E2_1562at 1823.783 kb on - strand, within Pf6N2E2_1562at 1823.783 kb on - strand, within Pf6N2E2_1562at 1823.785 kb on + strand, within Pf6N2E2_1562at 1823.786 kb on - strand, within Pf6N2E2_1562at 1823.826 kb on + strand, within Pf6N2E2_1562at 1823.851 kb on + strand, within Pf6N2E2_1562at 1823.851 kb on + strand, within Pf6N2E2_1562at 1823.851 kb on + strand, within Pf6N2E2_1562at 1823.851 kb on + strand, within Pf6N2E2_1562at 1823.851 kb on + strand, within Pf6N2E2_1562at 1823.851 kb on + strand, within Pf6N2E2_1562at 1823.852 kb on - strand, within Pf6N2E2_1562at 1823.852 kb on - strand, within Pf6N2E2_1562at 1823.852 kb on - strand, within Pf6N2E2_1562at 1823.852 kb on - strand, within Pf6N2E2_1562at 1823.852 kb on - strand, within Pf6N2E2_1562at 1823.907 kb on - strand, within Pf6N2E2_1562at 1823.907 kb on - strand, within Pf6N2E2_1562at 1824.064 kb on + strand, within Pf6N2E2_1562at 1824.064 kb on + strand, within Pf6N2E2_1562at 1824.065 kb on - strand, within Pf6N2E2_1562at 1824.065 kb on - strand, within Pf6N2E2_1562at 1824.110 kb on - strand, within Pf6N2E2_1562at 1824.276 kb on - strand, within Pf6N2E2_1562

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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1,821,903 - +0.0
1,821,903 - -0.7
1,822,028 - Pf6N2E2_1560 0.19 -0.7
1,822,373 + Pf6N2E2_1560 0.57 +0.3
1,822,373 + Pf6N2E2_1560 0.57 +0.3
1,822,558 + Pf6N2E2_1560 0.77 -0.7
1,822,558 + Pf6N2E2_1560 0.77 +1.8
1,822,703 + -0.7
1,822,730 + +0.3
1,822,795 - +0.3
1,822,799 + +0.3
1,822,799 + -0.2
1,822,886 + -0.4
1,822,887 - -0.7
1,822,887 - -0.7
1,823,027 + Pf6N2E2_1561 0.25 -1.3
1,823,027 + Pf6N2E2_1561 0.25 +0.3
1,823,027 + Pf6N2E2_1561 0.25 +0.3
1,823,090 + Pf6N2E2_1561 0.35 +0.3
1,823,179 + Pf6N2E2_1561 0.50 -2.1
1,823,180 - Pf6N2E2_1561 0.51 +0.3
1,823,225 - Pf6N2E2_1561 0.58 +0.3
1,823,279 + Pf6N2E2_1561 0.67 -0.9
1,823,279 + Pf6N2E2_1561 0.67 -1.3
1,823,280 - Pf6N2E2_1561 0.67 +0.3
1,823,342 + Pf6N2E2_1561 0.78 +0.3
1,823,342 + Pf6N2E2_1561 0.78 +0.3
1,823,342 + Pf6N2E2_1561 0.78 +0.3
1,823,342 + Pf6N2E2_1561 0.78 -1.5
1,823,343 - Pf6N2E2_1561 0.78 -1.7
1,823,343 - Pf6N2E2_1561 0.78 +0.3
1,823,343 - Pf6N2E2_1561 0.78 +0.3
1,823,343 - Pf6N2E2_1561 0.78 +0.3
1,823,475 - +0.3
1,823,650 + -1.7
1,823,651 - +0.3
1,823,651 - -0.7
1,823,666 + -0.9
1,823,782 + Pf6N2E2_1562 0.16 -0.7
1,823,782 + Pf6N2E2_1562 0.16 +0.3
1,823,782 + Pf6N2E2_1562 0.16 -0.7
1,823,783 - Pf6N2E2_1562 0.16 +1.1
1,823,783 - Pf6N2E2_1562 0.16 -2.5
1,823,785 + Pf6N2E2_1562 0.16 +0.3
1,823,786 - Pf6N2E2_1562 0.16 +0.3
1,823,826 + Pf6N2E2_1562 0.20 -1.3
1,823,851 + Pf6N2E2_1562 0.23 +0.3
1,823,851 + Pf6N2E2_1562 0.23 +0.3
1,823,851 + Pf6N2E2_1562 0.23 +0.3
1,823,851 + Pf6N2E2_1562 0.23 +0.3
1,823,851 + Pf6N2E2_1562 0.23 -1.3
1,823,851 + Pf6N2E2_1562 0.23 +0.3
1,823,852 - Pf6N2E2_1562 0.23 +0.3
1,823,852 - Pf6N2E2_1562 0.23 +0.3
1,823,852 - Pf6N2E2_1562 0.23 +0.3
1,823,852 - Pf6N2E2_1562 0.23 -0.7
1,823,852 - Pf6N2E2_1562 0.23 -0.7
1,823,907 - Pf6N2E2_1562 0.29 -0.3
1,823,907 - Pf6N2E2_1562 0.29 -2.7
1,824,064 + Pf6N2E2_1562 0.45 +0.3
1,824,064 + Pf6N2E2_1562 0.45 +0.3
1,824,065 - Pf6N2E2_1562 0.45 +0.3
1,824,065 - Pf6N2E2_1562 0.45 +0.3
1,824,110 - Pf6N2E2_1562 0.50 -0.7
1,824,276 - Pf6N2E2_1562 0.67 -0.3

Or see this region's nucleotide sequence