Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_149

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_148 and Pf6N2E2_149 are separated by 274 nucleotidesPf6N2E2_149 and Pf6N2E2_150 are separated by 155 nucleotidesPf6N2E2_150 and Pf6N2E2_151 are separated by 2 nucleotides Pf6N2E2_148 - Membrane fusion component of tripartite multidrug resistance system, at 203,029 to 204,225 _148 Pf6N2E2_149 - Inner membrane component of tripartite multidrug resistance system, at 204,500 to 206,029 _149 Pf6N2E2_150 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 206,185 to 206,934 _150 Pf6N2E2_151 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 206,937 to 207,440 _151 Position (kb) 204 205 206 207Strain fitness (log2 ratio) -2 -1 0 1 2at 203.676 kb on + strand, within Pf6N2E2_148at 203.677 kb on - strand, within Pf6N2E2_148at 203.702 kb on - strand, within Pf6N2E2_148at 203.863 kb on + strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.864 kb on - strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.881 kb on + strand, within Pf6N2E2_148at 203.882 kb on - strand, within Pf6N2E2_148at 203.936 kb on + strand, within Pf6N2E2_148at 204.137 kb on - strandat 204.137 kb on - strandat 204.137 kb on - strandat 204.703 kb on + strand, within Pf6N2E2_149at 204.790 kb on + strand, within Pf6N2E2_149at 204.790 kb on + strand, within Pf6N2E2_149at 204.791 kb on - strand, within Pf6N2E2_149at 204.828 kb on + strand, within Pf6N2E2_149at 204.829 kb on - strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.992 kb on + strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 204.993 kb on - strand, within Pf6N2E2_149at 205.107 kb on - strand, within Pf6N2E2_149at 205.107 kb on - strand, within Pf6N2E2_149at 205.340 kb on + strand, within Pf6N2E2_149at 205.341 kb on - strand, within Pf6N2E2_149at 205.478 kb on + strand, within Pf6N2E2_149at 205.479 kb on - strand, within Pf6N2E2_149at 205.479 kb on - strand, within Pf6N2E2_149at 205.479 kb on - strand, within Pf6N2E2_149at 205.787 kb on + strand, within Pf6N2E2_149at 205.787 kb on + strand, within Pf6N2E2_149at 205.788 kb on - strand, within Pf6N2E2_149at 205.788 kb on - strand, within Pf6N2E2_149at 205.788 kb on - strand, within Pf6N2E2_149at 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.904 kb on + strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.905 kb on - strandat 205.925 kb on + strandat 205.925 kb on + strandat 205.926 kb on - strandat 205.926 kb on - strandat 205.926 kb on - strandat 205.926 kb on - strandat 205.926 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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203,676 + Pf6N2E2_148 0.54 +0.3
203,677 - Pf6N2E2_148 0.54 +0.8
203,702 - Pf6N2E2_148 0.56 +0.3
203,863 + Pf6N2E2_148 0.70 +0.1
203,864 - Pf6N2E2_148 0.70 +0.3
203,864 - Pf6N2E2_148 0.70 +0.3
203,881 + Pf6N2E2_148 0.71 +0.3
203,881 + Pf6N2E2_148 0.71 +0.7
203,881 + Pf6N2E2_148 0.71 +1.3
203,882 - Pf6N2E2_148 0.71 +0.3
203,936 + Pf6N2E2_148 0.76 +0.3
204,137 - +0.9
204,137 - -0.2
204,137 - +0.6
204,703 + Pf6N2E2_149 0.13 +0.3
204,790 + Pf6N2E2_149 0.19 -0.7
204,790 + Pf6N2E2_149 0.19 -0.7
204,791 - Pf6N2E2_149 0.19 +0.3
204,828 + Pf6N2E2_149 0.21 -0.1
204,829 - Pf6N2E2_149 0.22 +2.3
204,992 + Pf6N2E2_149 0.32 +0.1
204,992 + Pf6N2E2_149 0.32 +0.3
204,992 + Pf6N2E2_149 0.32 +0.8
204,992 + Pf6N2E2_149 0.32 +0.3
204,992 + Pf6N2E2_149 0.32 -0.7
204,992 + Pf6N2E2_149 0.32 -0.7
204,993 - Pf6N2E2_149 0.32 +0.3
204,993 - Pf6N2E2_149 0.32 -0.7
204,993 - Pf6N2E2_149 0.32 +0.3
205,107 - Pf6N2E2_149 0.40 -0.7
205,107 - Pf6N2E2_149 0.40 +1.6
205,340 + Pf6N2E2_149 0.55 -0.3
205,341 - Pf6N2E2_149 0.55 -0.7
205,478 + Pf6N2E2_149 0.64 +0.1
205,479 - Pf6N2E2_149 0.64 -1.7
205,479 - Pf6N2E2_149 0.64 +0.3
205,479 - Pf6N2E2_149 0.64 +0.2
205,787 + Pf6N2E2_149 0.84 -0.7
205,787 + Pf6N2E2_149 0.84 -0.7
205,788 - Pf6N2E2_149 0.84 +0.3
205,788 - Pf6N2E2_149 0.84 -0.7
205,788 - Pf6N2E2_149 0.84 +0.3
205,904 + +0.7
205,904 + +0.3
205,904 + +0.3
205,904 + +0.3
205,904 + +0.4
205,904 + +0.2
205,904 + +0.3
205,904 + -0.7
205,904 + -0.7
205,904 + +0.3
205,904 + -0.1
205,904 + +0.3
205,904 + +0.3
205,905 - -0.1
205,905 - -2.0
205,905 - -0.3
205,905 - -0.8
205,905 - +0.5
205,905 - +1.0
205,905 - +0.3
205,905 - -0.7
205,905 - -1.7
205,905 - -0.4
205,905 - -0.7
205,905 - -0.7
205,925 + +0.3
205,925 + -0.9
205,926 - +0.3
205,926 - +0.2
205,926 - -0.7
205,926 - -1.3
205,926 - -0.7

Or see this region's nucleotide sequence