Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1423 and Pf6N2E2_1424 are separated by 517 nucleotides Pf6N2E2_1424 and Pf6N2E2_1425 are separated by 48 nucleotides
Pf6N2E2_1423 - Gamma-aminobutyrate permease, at 1,670,500 to 1,671,900
_1423
Pf6N2E2_1424 - transcriptional regulator, TetR family, at 1,672,418 to 1,673,029
_1424
Pf6N2E2_1425 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 1,673,078 to 1,674,181
_1425
Position (kb)
1672
1673
1674 Strain fitness (log2 ratio)
-2
-1
0
1 at 1671.599 kb on + strand, within Pf6N2E2_1423 at 1671.651 kb on - strand, within Pf6N2E2_1423 at 1671.651 kb on - strand, within Pf6N2E2_1423 at 1671.740 kb on + strand, within Pf6N2E2_1423 at 1671.741 kb on - strand, within Pf6N2E2_1423 at 1672.151 kb on + strand at 1672.170 kb on - strand at 1672.170 kb on - strand at 1672.307 kb on + strand at 1672.389 kb on + strand at 1672.390 kb on - strand at 1672.390 kb on - strand at 1672.569 kb on - strand, within Pf6N2E2_1424 at 1672.686 kb on - strand, within Pf6N2E2_1424 at 1672.994 kb on - strand at 1673.147 kb on + strand at 1673.148 kb on - strand at 1673.182 kb on + strand at 1673.182 kb on + strand at 1673.182 kb on + strand at 1673.183 kb on - strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.298 kb on + strand, within Pf6N2E2_1425 at 1673.298 kb on + strand, within Pf6N2E2_1425 at 1673.298 kb on + strand, within Pf6N2E2_1425 at 1673.299 kb on - strand, within Pf6N2E2_1425 at 1673.299 kb on - strand, within Pf6N2E2_1425 at 1673.313 kb on + strand, within Pf6N2E2_1425 at 1673.523 kb on + strand, within Pf6N2E2_1425 at 1673.524 kb on - strand, within Pf6N2E2_1425 at 1673.684 kb on - strand, within Pf6N2E2_1425 at 1673.713 kb on + strand, within Pf6N2E2_1425 at 1673.714 kb on - strand, within Pf6N2E2_1425 at 1673.714 kb on - strand, within Pf6N2E2_1425 at 1673.761 kb on + strand, within Pf6N2E2_1425 at 1673.763 kb on + strand, within Pf6N2E2_1425 at 1673.763 kb on + strand, within Pf6N2E2_1425
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 1,671,599 + Pf6N2E2_1423 0.78 +0.3 1,671,651 - Pf6N2E2_1423 0.82 -0.7 1,671,651 - Pf6N2E2_1423 0.82 -1.3 1,671,740 + Pf6N2E2_1423 0.89 +0.3 1,671,741 - Pf6N2E2_1423 0.89 -0.1 1,672,151 + -1.3 1,672,170 - -1.8 1,672,170 - +0.2 1,672,307 + +0.2 1,672,389 + +0.2 1,672,390 - +0.2 1,672,390 - +0.2 1,672,569 - Pf6N2E2_1424 0.25 -2.6 1,672,686 - Pf6N2E2_1424 0.44 +0.2 1,672,994 - -0.8 1,673,147 + +0.2 1,673,148 - -1.2 1,673,182 + +0.3 1,673,182 + +0.4 1,673,182 + -0.2 1,673,183 - +0.3 1,673,184 + +0.3 1,673,184 + +0.3 1,673,184 + +0.3 1,673,184 + +0.3 1,673,184 + -1.3 1,673,184 + +0.3 1,673,184 + +0.3 1,673,184 + -0.7 1,673,184 + -0.7 1,673,184 + -0.7 1,673,184 + -0.5 1,673,185 - +0.3 1,673,185 - -0.7 1,673,185 - +0.3 1,673,185 - +0.3 1,673,185 - -0.7 1,673,185 - -0.5 1,673,185 - +0.3 1,673,185 - +0.3 1,673,185 - +0.3 1,673,185 - +0.3 1,673,185 - +0.3 1,673,185 - +0.3 1,673,298 + Pf6N2E2_1425 0.20 +0.3 1,673,298 + Pf6N2E2_1425 0.20 +0.3 1,673,298 + Pf6N2E2_1425 0.20 +0.3 1,673,299 - Pf6N2E2_1425 0.20 +0.3 1,673,299 - Pf6N2E2_1425 0.20 +0.3 1,673,313 + Pf6N2E2_1425 0.21 +0.3 1,673,523 + Pf6N2E2_1425 0.40 +0.3 1,673,524 - Pf6N2E2_1425 0.40 +0.3 1,673,684 - Pf6N2E2_1425 0.55 +0.3 1,673,713 + Pf6N2E2_1425 0.58 +0.3 1,673,714 - Pf6N2E2_1425 0.58 +0.3 1,673,714 - Pf6N2E2_1425 0.58 +0.3 1,673,761 + Pf6N2E2_1425 0.62 +0.3 1,673,763 + Pf6N2E2_1425 0.62 +0.3 1,673,763 + Pf6N2E2_1425 0.62 -0.2
Or see this region's nucleotide sequence