Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1411 and Pf6N2E2_1412 overlap by 15 nucleotides Pf6N2E2_1412 and Pf6N2E2_1413 are separated by 53 nucleotides Pf6N2E2_1413 and Pf6N2E2_1414 are separated by 158 nucleotides Pf6N2E2_1414 and Pf6N2E2_1415 overlap by 18 nucleotides
Pf6N2E2_1411 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 1,656,648 to 1,657,742
_1411
Pf6N2E2_1412 - hypothetical protein, at 1,657,728 to 1,658,063
_1412
Pf6N2E2_1413 - putative membrane protein, at 1,658,117 to 1,658,560
_1413
Pf6N2E2_1414 - hypothetical protein, at 1,658,719 to 1,658,901
_1414
Pf6N2E2_1415 - mannuronan C-5-epimerase, putative, at 1,658,884 to 1,664,496
_1415
Position (kb)
1657
1658
1659 Strain fitness (log2 ratio)
-1
0
1 at 1656.988 kb on + strand, within Pf6N2E2_1411 at 1656.989 kb on - strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.014 kb on + strand, within Pf6N2E2_1411 at 1657.015 kb on - strand at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.015 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.019 kb on - strand, within Pf6N2E2_1411 at 1657.037 kb on + strand, within Pf6N2E2_1411 at 1657.086 kb on - strand, within Pf6N2E2_1411 at 1657.174 kb on + strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.175 kb on - strand, within Pf6N2E2_1411 at 1657.300 kb on + strand, within Pf6N2E2_1411 at 1657.301 kb on - strand, within Pf6N2E2_1411 at 1657.615 kb on + strand, within Pf6N2E2_1411 at 1657.616 kb on - strand, within Pf6N2E2_1411 at 1657.616 kb on - strand, within Pf6N2E2_1411 at 1657.753 kb on + strand at 1657.754 kb on - strand at 1657.754 kb on - strand at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.935 kb on - strand, within Pf6N2E2_1412 at 1657.941 kb on - strand, within Pf6N2E2_1412 at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.130 kb on + strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.131 kb on - strand at 1658.683 kb on - strand at 1658.817 kb on + strand, within Pf6N2E2_1414 at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.995 kb on + strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1658.996 kb on - strand at 1659.014 kb on - strand at 1659.014 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 1,656,988 + Pf6N2E2_1411 0.31 -0.5 1,656,989 - Pf6N2E2_1411 0.31 +0.3 1,657,014 + Pf6N2E2_1411 0.33 +0.3 1,657,014 + Pf6N2E2_1411 0.33 +0.3 1,657,014 + Pf6N2E2_1411 0.33 +0.3 1,657,015 - -0.7 1,657,015 - Pf6N2E2_1411 0.34 +0.3 1,657,015 - Pf6N2E2_1411 0.34 -1.1 1,657,015 - Pf6N2E2_1411 0.34 -0.5 1,657,019 - Pf6N2E2_1411 0.34 -0.3 1,657,019 - Pf6N2E2_1411 0.34 +0.3 1,657,019 - Pf6N2E2_1411 0.34 +0.3 1,657,037 + Pf6N2E2_1411 0.36 -0.1 1,657,086 - Pf6N2E2_1411 0.40 +0.3 1,657,174 + Pf6N2E2_1411 0.48 +0.3 1,657,175 - Pf6N2E2_1411 0.48 -0.7 1,657,175 - Pf6N2E2_1411 0.48 +0.3 1,657,175 - Pf6N2E2_1411 0.48 -0.5 1,657,175 - Pf6N2E2_1411 0.48 +0.3 1,657,175 - Pf6N2E2_1411 0.48 +0.3 1,657,175 - Pf6N2E2_1411 0.48 -0.7 1,657,175 - Pf6N2E2_1411 0.48 +0.3 1,657,300 + Pf6N2E2_1411 0.60 -1.7 1,657,301 - Pf6N2E2_1411 0.60 -0.7 1,657,615 + Pf6N2E2_1411 0.88 +0.3 1,657,616 - Pf6N2E2_1411 0.88 +0.3 1,657,616 - Pf6N2E2_1411 0.88 +0.3 1,657,753 + -0.6 1,657,754 - -0.2 1,657,754 - -0.7 1,657,935 - Pf6N2E2_1412 0.62 -1.3 1,657,935 - Pf6N2E2_1412 0.62 -0.7 1,657,935 - Pf6N2E2_1412 0.62 +0.3 1,657,935 - Pf6N2E2_1412 0.62 +0.3 1,657,941 - Pf6N2E2_1412 0.63 -0.7 1,658,130 + +0.3 1,658,130 + +0.3 1,658,130 + -0.0 1,658,131 - +0.3 1,658,131 - +0.3 1,658,131 - -1.1 1,658,131 - +0.3 1,658,683 - -1.3 1,658,817 + Pf6N2E2_1414 0.54 +0.3 1,658,995 + +0.3 1,658,995 + +0.3 1,658,995 + +0.3 1,658,995 + +0.3 1,658,995 + +0.3 1,658,995 + +0.3 1,658,995 + +0.3 1,658,996 - +0.3 1,658,996 - -0.7 1,658,996 - +0.3 1,658,996 - +0.3 1,658,996 - -0.7 1,659,014 - +0.3 1,659,014 - +0.3
Or see this region's nucleotide sequence