Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1412

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1411 and Pf6N2E2_1412 overlap by 15 nucleotidesPf6N2E2_1412 and Pf6N2E2_1413 are separated by 53 nucleotidesPf6N2E2_1413 and Pf6N2E2_1414 are separated by 158 nucleotidesPf6N2E2_1414 and Pf6N2E2_1415 overlap by 18 nucleotides Pf6N2E2_1411 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 1,656,648 to 1,657,742 _1411 Pf6N2E2_1412 - hypothetical protein, at 1,657,728 to 1,658,063 _1412 Pf6N2E2_1413 - putative membrane protein, at 1,658,117 to 1,658,560 _1413 Pf6N2E2_1414 - hypothetical protein, at 1,658,719 to 1,658,901 _1414 Pf6N2E2_1415 - mannuronan C-5-epimerase, putative, at 1,658,884 to 1,664,496 _1415 Position (kb) 1657 1658 1659Strain fitness (log2 ratio) -1 0 1at 1656.988 kb on + strand, within Pf6N2E2_1411at 1656.989 kb on - strand, within Pf6N2E2_1411at 1657.014 kb on + strand, within Pf6N2E2_1411at 1657.014 kb on + strand, within Pf6N2E2_1411at 1657.014 kb on + strand, within Pf6N2E2_1411at 1657.015 kb on - strandat 1657.015 kb on - strand, within Pf6N2E2_1411at 1657.015 kb on - strand, within Pf6N2E2_1411at 1657.015 kb on - strand, within Pf6N2E2_1411at 1657.019 kb on - strand, within Pf6N2E2_1411at 1657.019 kb on - strand, within Pf6N2E2_1411at 1657.019 kb on - strand, within Pf6N2E2_1411at 1657.037 kb on + strand, within Pf6N2E2_1411at 1657.086 kb on - strand, within Pf6N2E2_1411at 1657.174 kb on + strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.175 kb on - strand, within Pf6N2E2_1411at 1657.300 kb on + strand, within Pf6N2E2_1411at 1657.301 kb on - strand, within Pf6N2E2_1411at 1657.615 kb on + strand, within Pf6N2E2_1411at 1657.616 kb on - strand, within Pf6N2E2_1411at 1657.616 kb on - strand, within Pf6N2E2_1411at 1657.753 kb on + strandat 1657.754 kb on - strandat 1657.754 kb on - strandat 1657.935 kb on - strand, within Pf6N2E2_1412at 1657.935 kb on - strand, within Pf6N2E2_1412at 1657.935 kb on - strand, within Pf6N2E2_1412at 1657.935 kb on - strand, within Pf6N2E2_1412at 1657.941 kb on - strand, within Pf6N2E2_1412at 1658.130 kb on + strandat 1658.130 kb on + strandat 1658.130 kb on + strandat 1658.131 kb on - strandat 1658.131 kb on - strandat 1658.131 kb on - strandat 1658.131 kb on - strandat 1658.683 kb on - strandat 1658.817 kb on + strand, within Pf6N2E2_1414at 1658.995 kb on + strandat 1658.995 kb on + strandat 1658.995 kb on + strandat 1658.995 kb on + strandat 1658.995 kb on + strandat 1658.995 kb on + strandat 1658.995 kb on + strandat 1658.996 kb on - strandat 1658.996 kb on - strandat 1658.996 kb on - strandat 1658.996 kb on - strandat 1658.996 kb on - strandat 1659.014 kb on - strandat 1659.014 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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1,656,988 + Pf6N2E2_1411 0.31 -0.5
1,656,989 - Pf6N2E2_1411 0.31 +0.3
1,657,014 + Pf6N2E2_1411 0.33 +0.3
1,657,014 + Pf6N2E2_1411 0.33 +0.3
1,657,014 + Pf6N2E2_1411 0.33 +0.3
1,657,015 - -0.7
1,657,015 - Pf6N2E2_1411 0.34 +0.3
1,657,015 - Pf6N2E2_1411 0.34 -1.1
1,657,015 - Pf6N2E2_1411 0.34 -0.5
1,657,019 - Pf6N2E2_1411 0.34 -0.3
1,657,019 - Pf6N2E2_1411 0.34 +0.3
1,657,019 - Pf6N2E2_1411 0.34 +0.3
1,657,037 + Pf6N2E2_1411 0.36 -0.1
1,657,086 - Pf6N2E2_1411 0.40 +0.3
1,657,174 + Pf6N2E2_1411 0.48 +0.3
1,657,175 - Pf6N2E2_1411 0.48 -0.7
1,657,175 - Pf6N2E2_1411 0.48 +0.3
1,657,175 - Pf6N2E2_1411 0.48 -0.5
1,657,175 - Pf6N2E2_1411 0.48 +0.3
1,657,175 - Pf6N2E2_1411 0.48 +0.3
1,657,175 - Pf6N2E2_1411 0.48 -0.7
1,657,175 - Pf6N2E2_1411 0.48 +0.3
1,657,300 + Pf6N2E2_1411 0.60 -1.7
1,657,301 - Pf6N2E2_1411 0.60 -0.7
1,657,615 + Pf6N2E2_1411 0.88 +0.3
1,657,616 - Pf6N2E2_1411 0.88 +0.3
1,657,616 - Pf6N2E2_1411 0.88 +0.3
1,657,753 + -0.6
1,657,754 - -0.2
1,657,754 - -0.7
1,657,935 - Pf6N2E2_1412 0.62 -1.3
1,657,935 - Pf6N2E2_1412 0.62 -0.7
1,657,935 - Pf6N2E2_1412 0.62 +0.3
1,657,935 - Pf6N2E2_1412 0.62 +0.3
1,657,941 - Pf6N2E2_1412 0.63 -0.7
1,658,130 + +0.3
1,658,130 + +0.3
1,658,130 + -0.0
1,658,131 - +0.3
1,658,131 - +0.3
1,658,131 - -1.1
1,658,131 - +0.3
1,658,683 - -1.3
1,658,817 + Pf6N2E2_1414 0.54 +0.3
1,658,995 + +0.3
1,658,995 + +0.3
1,658,995 + +0.3
1,658,995 + +0.3
1,658,995 + +0.3
1,658,995 + +0.3
1,658,995 + +0.3
1,658,996 - +0.3
1,658,996 - -0.7
1,658,996 - +0.3
1,658,996 - +0.3
1,658,996 - -0.7
1,659,014 - +0.3
1,659,014 - +0.3

Or see this region's nucleotide sequence