Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1146 and Pf6N2E2_1147 are separated by 13 nucleotides Pf6N2E2_1147 and Pf6N2E2_1148 are separated by 231 nucleotides
Pf6N2E2_1146 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,343,285 to 1,344,412
_1146
Pf6N2E2_1147 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,344,426 to 1,345,199
_1147
Pf6N2E2_1148 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,345,431 to 1,346,582
_1148
Position (kb)
1344
1345
1346 Strain fitness (log2 ratio)
-2
-1
0
1 at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.973 kb on - strand, within Pf6N2E2_1146 at 1344.068 kb on + strand, within Pf6N2E2_1146 at 1344.069 kb on - strand, within Pf6N2E2_1146 at 1344.179 kb on + strand, within Pf6N2E2_1146 at 1344.362 kb on + strand at 1344.362 kb on + strand at 1344.362 kb on + strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.660 kb on + strand, within Pf6N2E2_1147 at 1344.661 kb on - strand, within Pf6N2E2_1147 at 1345.031 kb on + strand, within Pf6N2E2_1147 at 1345.032 kb on - strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.194 kb on - strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.198 kb on - strand at 1345.423 kb on + strand at 1345.423 kb on + strand at 1345.424 kb on - strand at 1345.441 kb on + strand at 1345.476 kb on + strand at 1345.477 kb on - strand at 1345.495 kb on + strand at 1345.495 kb on + strand at 1345.495 kb on + strand at 1345.496 kb on - strand at 1345.496 kb on - strand at 1345.496 kb on - strand at 1345.522 kb on + strand at 1345.525 kb on + strand at 1345.526 kb on - strand at 1345.526 kb on - strand at 1345.789 kb on + strand, within Pf6N2E2_1148 at 1346.012 kb on + strand, within Pf6N2E2_1148
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 1,343,483 + Pf6N2E2_1146 0.18 +0.2 1,343,483 + Pf6N2E2_1146 0.18 +0.2 1,343,720 + Pf6N2E2_1146 0.39 -1.2 1,343,720 + Pf6N2E2_1146 0.39 +0.2 1,343,720 + Pf6N2E2_1146 0.39 -0.8 1,343,720 + Pf6N2E2_1146 0.39 +0.2 1,343,720 + Pf6N2E2_1146 0.39 +0.2 1,343,721 - Pf6N2E2_1146 0.39 -0.8 1,343,721 - Pf6N2E2_1146 0.39 +0.2 1,343,972 + Pf6N2E2_1146 0.61 +0.2 1,343,972 + Pf6N2E2_1146 0.61 -0.8 1,343,973 - Pf6N2E2_1146 0.61 +0.2 1,344,068 + Pf6N2E2_1146 0.69 +0.2 1,344,069 - Pf6N2E2_1146 0.70 +0.2 1,344,179 + Pf6N2E2_1146 0.79 +0.2 1,344,362 + +0.2 1,344,362 + +0.2 1,344,362 + +1.2 1,344,363 - -1.3 1,344,363 - -1.8 1,344,363 - -0.8 1,344,363 - +0.2 1,344,363 - -0.8 1,344,433 - -0.8 1,344,433 - -2.3 1,344,433 - -0.8 1,344,660 + Pf6N2E2_1147 0.30 -2.3 1,344,661 - Pf6N2E2_1147 0.30 +1.2 1,345,031 + Pf6N2E2_1147 0.78 +0.2 1,345,032 - +0.2 1,345,193 + -0.8 1,345,193 + +0.2 1,345,193 + +0.2 1,345,194 - +0.2 1,345,197 + -0.8 1,345,197 + -0.8 1,345,197 + -0.7 1,345,197 + +0.2 1,345,198 - +0.2 1,345,423 + -0.8 1,345,423 + +0.2 1,345,424 - -0.8 1,345,441 + -0.4 1,345,476 + +0.2 1,345,477 - -0.8 1,345,495 + -1.1 1,345,495 + +0.2 1,345,495 + -0.3 1,345,496 - +0.2 1,345,496 - +0.1 1,345,496 - -0.2 1,345,522 + -0.2 1,345,525 + -0.8 1,345,526 - -0.8 1,345,526 - +0.2 1,345,789 + Pf6N2E2_1148 0.31 +0.2 1,346,012 + Pf6N2E2_1148 0.50 +0.2
Or see this region's nucleotide sequence