Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1026 and Pf6N2E2_1027 overlap by 4 nucleotides Pf6N2E2_1027 and Pf6N2E2_1028 are separated by 195 nucleotides Pf6N2E2_1028 and Pf6N2E2_1029 are separated by 126 nucleotides
Pf6N2E2_1026 - Rhodanese-related sulfurtransferase, at 1,199,480 to 1,201,063
_1026
Pf6N2E2_1027 - Cysteine dioxygenase (EC 1.13.11.20), at 1,201,060 to 1,201,695
_1027
Pf6N2E2_1028 - Transcriptional regulator, LysR family, at 1,201,891 to 1,202,796
_1028
Pf6N2E2_1029 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 1,202,923 to 1,203,717
_1029
Position (kb)
1201
1202
1203 Strain fitness (log2 ratio)
-1
0
1 at 1200.903 kb on + strand, within Pf6N2E2_1026 at 1201.052 kb on + strand at 1201.053 kb on - strand at 1201.064 kb on - strand at 1201.157 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.481 kb on + strand, within Pf6N2E2_1027 at 1201.482 kb on - strand at 1201.482 kb on - strand, within Pf6N2E2_1027 at 1201.832 kb on + strand at 1201.864 kb on - strand at 1201.864 kb on - strand at 1201.864 kb on - strand at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.449 kb on + strand, within Pf6N2E2_1028 at 1202.704 kb on + strand, within Pf6N2E2_1028 at 1202.881 kb on - strand at 1202.899 kb on + strand at 1202.946 kb on + strand at 1203.058 kb on + strand, within Pf6N2E2_1029 at 1203.058 kb on + strand, within Pf6N2E2_1029 at 1203.058 kb on + strand, within Pf6N2E2_1029 at 1203.059 kb on - strand, within Pf6N2E2_1029 at 1203.059 kb on - strand, within Pf6N2E2_1029 at 1203.059 kb on - strand, within Pf6N2E2_1029 at 1203.193 kb on - strand, within Pf6N2E2_1029 at 1203.220 kb on + strand, within Pf6N2E2_1029 at 1203.446 kb on + strand, within Pf6N2E2_1029 at 1203.446 kb on + strand, within Pf6N2E2_1029 at 1203.447 kb on - strand, within Pf6N2E2_1029 at 1203.746 kb on - strand at 1203.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 1,200,903 + Pf6N2E2_1026 0.90 +0.3 1,201,052 + -0.3 1,201,053 - +0.3 1,201,064 - -0.6 1,201,157 + Pf6N2E2_1027 0.15 +0.3 1,201,481 + Pf6N2E2_1027 0.66 +0.3 1,201,481 + Pf6N2E2_1027 0.66 -0.7 1,201,481 + Pf6N2E2_1027 0.66 +0.3 1,201,482 - +0.3 1,201,482 - Pf6N2E2_1027 0.66 -0.7 1,201,832 + -1.1 1,201,864 - +0.7 1,201,864 - -0.8 1,201,864 - +0.3 1,202,449 + Pf6N2E2_1028 0.62 -0.7 1,202,449 + Pf6N2E2_1028 0.62 +0.3 1,202,449 + Pf6N2E2_1028 0.62 -0.7 1,202,704 + Pf6N2E2_1028 0.90 -0.7 1,202,881 - +0.3 1,202,899 + +0.3 1,202,946 + +0.3 1,203,058 + Pf6N2E2_1029 0.17 -0.7 1,203,058 + Pf6N2E2_1029 0.17 +0.3 1,203,058 + Pf6N2E2_1029 0.17 +0.3 1,203,059 - Pf6N2E2_1029 0.17 +0.8 1,203,059 - Pf6N2E2_1029 0.17 +0.3 1,203,059 - Pf6N2E2_1029 0.17 +0.3 1,203,193 - Pf6N2E2_1029 0.34 -0.5 1,203,220 + Pf6N2E2_1029 0.37 +0.3 1,203,446 + Pf6N2E2_1029 0.66 +0.8 1,203,446 + Pf6N2E2_1029 0.66 +0.3 1,203,447 - Pf6N2E2_1029 0.66 +0.3 1,203,746 - -0.3 1,203,760 + +0.3
Or see this region's nucleotide sequence