Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4686 and Pf6N2E2_4687 are separated by 144 nucleotides Pf6N2E2_4687 and Pf6N2E2_4688 are separated by 300 nucleotides
Pf6N2E2_4686 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,420,314 to 5,421,417
_4686
Pf6N2E2_4687 - FIG00953780: hypothetical protein, at 5,421,562 to 5,421,825
_4687
Pf6N2E2_4688 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 5,422,126 to 5,423,289
_4688
Position (kb)
5421
5422 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5420.562 kb on + strand, within Pf6N2E2_4686 at 5420.599 kb on - strand, within Pf6N2E2_4686 at 5420.781 kb on + strand, within Pf6N2E2_4686 at 5420.915 kb on - strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.059 kb on - strand, within Pf6N2E2_4686 at 5421.104 kb on - strand, within Pf6N2E2_4686 at 5421.104 kb on - strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.128 kb on - strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.207 kb on - strand, within Pf6N2E2_4686 at 5421.254 kb on - strand, within Pf6N2E2_4686 at 5421.385 kb on - strand at 5421.415 kb on + strand at 5421.416 kb on - strand at 5421.479 kb on + strand at 5421.480 kb on - strand at 5421.488 kb on + strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.642 kb on + strand, within Pf6N2E2_4687 at 5421.643 kb on - strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.757 kb on + strand, within Pf6N2E2_4687 at 5421.818 kb on + strand at 5421.818 kb on + strand at 5421.819 kb on - strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.923 kb on - strand at 5421.923 kb on - strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.283 kb on - strand, within Pf6N2E2_4688 at 5422.627 kb on + strand, within Pf6N2E2_4688 at 5422.627 kb on + strand, within Pf6N2E2_4688 at 5422.677 kb on - strand, within Pf6N2E2_4688
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 5,420,562 + Pf6N2E2_4686 0.22 -0.5 5,420,599 - Pf6N2E2_4686 0.26 -1.1 5,420,781 + Pf6N2E2_4686 0.42 -0.5 5,420,915 - Pf6N2E2_4686 0.54 -0.2 5,421,058 + Pf6N2E2_4686 0.67 +0.5 5,421,058 + Pf6N2E2_4686 0.67 +0.5 5,421,058 + Pf6N2E2_4686 0.67 +0.5 5,421,058 + Pf6N2E2_4686 0.67 +0.5 5,421,059 - Pf6N2E2_4686 0.67 +0.3 5,421,104 - Pf6N2E2_4686 0.72 -1.1 5,421,104 - Pf6N2E2_4686 0.72 -0.5 5,421,127 + Pf6N2E2_4686 0.74 +0.0 5,421,127 + Pf6N2E2_4686 0.74 -0.5 5,421,128 - Pf6N2E2_4686 0.74 -1.1 5,421,130 + Pf6N2E2_4686 0.74 +0.5 5,421,130 + Pf6N2E2_4686 0.74 +0.5 5,421,130 + Pf6N2E2_4686 0.74 +0.5 5,421,130 + Pf6N2E2_4686 0.74 -0.5 5,421,130 + Pf6N2E2_4686 0.74 -1.1 5,421,130 + Pf6N2E2_4686 0.74 -0.5 5,421,131 - Pf6N2E2_4686 0.74 -0.5 5,421,131 - Pf6N2E2_4686 0.74 -0.9 5,421,207 - Pf6N2E2_4686 0.81 -0.1 5,421,254 - Pf6N2E2_4686 0.85 -0.8 5,421,385 - -2.5 5,421,415 + +2.5 5,421,416 - -1.1 5,421,479 + +0.5 5,421,480 - -0.5 5,421,488 + -1.1 5,421,489 - +0.5 5,421,489 - -1.1 5,421,489 - -0.5 5,421,489 - +0.5 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 -1.1 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 -0.5 5,421,642 + Pf6N2E2_4687 0.30 +0.5 5,421,643 - Pf6N2E2_4687 0.31 -0.5 5,421,659 + Pf6N2E2_4687 0.37 +0.5 5,421,659 + Pf6N2E2_4687 0.37 -1.5 5,421,659 + Pf6N2E2_4687 0.37 -0.5 5,421,659 + Pf6N2E2_4687 0.37 -0.2 5,421,659 + Pf6N2E2_4687 0.37 +0.5 5,421,660 - Pf6N2E2_4687 0.37 +0.5 5,421,660 - Pf6N2E2_4687 0.37 -1.6 5,421,660 - Pf6N2E2_4687 0.37 -0.5 5,421,660 - Pf6N2E2_4687 0.37 -2.2 5,421,660 - Pf6N2E2_4687 0.37 -0.8 5,421,660 - Pf6N2E2_4687 0.37 -1.1 5,421,660 - Pf6N2E2_4687 0.37 -1.1 5,421,757 + Pf6N2E2_4687 0.74 -1.5 5,421,818 + -0.5 5,421,818 + +0.5 5,421,819 - -0.5 5,421,922 + +0.5 5,421,922 + +0.5 5,421,922 + +0.3 5,421,923 - -1.1 5,421,923 - -0.2 5,422,175 + -0.4 5,422,175 + -1.8 5,422,175 + -0.7 5,422,175 + +0.5 5,422,175 + -0.5 5,422,175 + -0.5 5,422,175 + +1.1 5,422,175 + +2.5 5,422,175 + -2.5 5,422,175 + -0.5 5,422,175 + -0.5 5,422,176 - -0.3 5,422,176 - -0.5 5,422,176 - -0.8 5,422,176 - -0.5 5,422,176 - -0.5 5,422,176 - +0.5 5,422,176 - +0.5 5,422,176 - -0.1 5,422,176 - -0.5 5,422,176 - +0.5 5,422,176 - +0.5 5,422,176 - -1.1 5,422,176 - -1.7 5,422,283 - Pf6N2E2_4688 0.13 -0.5 5,422,627 + Pf6N2E2_4688 0.43 -0.5 5,422,627 + Pf6N2E2_4688 0.43 +0.2 5,422,677 - Pf6N2E2_4688 0.47 +0.5
Or see this region's nucleotide sequence