Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2583 and Pf6N2E2_2584 are separated by 9 nucleotides Pf6N2E2_2584 and Pf6N2E2_2585 are separated by 232 nucleotides Pf6N2E2_2585 and Pf6N2E2_2586 are separated by 88 nucleotides Pf6N2E2_2586 and Pf6N2E2_2587 are separated by 31 nucleotides
Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627
_2583
Pf6N2E2_2584 - Flagellar biosynthesis protein FliL, at 3,041,637 to 3,042,140
_2584
Pf6N2E2_2585 - Flagellar hook-length control protein FliK, at 3,042,373 to 3,043,746
_2585
Pf6N2E2_2586 - Hpt domain protein, at 3,043,835 to 3,044,179
_2586
Pf6N2E2_2587 - Serine phosphatase RsbU, regulator of sigma subunit, at 3,044,211 to 3,045,917
_2587
Position (kb)
3042
3043
3044 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3041.515 kb on + strand, within Pf6N2E2_2583 at 3041.639 kb on - strand at 3041.639 kb on - strand at 3041.647 kb on - strand at 3041.870 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3042.019 kb on + strand, within Pf6N2E2_2584 at 3042.019 kb on + strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.114 kb on + strand at 3042.114 kb on + strand at 3042.146 kb on + strand at 3042.147 kb on - strand at 3042.157 kb on + strand at 3042.201 kb on + strand at 3042.304 kb on + strand at 3042.305 kb on - strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.380 kb on + strand at 3042.380 kb on + strand at 3042.380 kb on + strand at 3042.381 kb on - strand at 3042.381 kb on - strand at 3042.383 kb on + strand at 3042.384 kb on - strand at 3042.417 kb on + strand at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.773 kb on + strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.774 kb on - strand, within Pf6N2E2_2585 at 3042.825 kb on + strand, within Pf6N2E2_2585 at 3042.826 kb on - strand, within Pf6N2E2_2585 at 3042.864 kb on + strand, within Pf6N2E2_2585 at 3042.865 kb on - strand, within Pf6N2E2_2585 at 3042.865 kb on - strand, within Pf6N2E2_2585 at 3043.549 kb on - strand, within Pf6N2E2_2585 at 3043.650 kb on + strand at 3043.728 kb on + strand at 3043.728 kb on + strand at 3043.729 kb on - strand at 3043.729 kb on - strand at 3043.729 kb on - strand at 3043.774 kb on + strand at 3043.775 kb on - strand at 3043.775 kb on - strand at 3043.775 kb on - strand at 3043.775 kb on - strand at 3043.779 kb on - strand at 3043.779 kb on - strand at 3043.881 kb on + strand, within Pf6N2E2_2586 at 3043.882 kb on - strand, within Pf6N2E2_2586 at 3044.018 kb on - strand, within Pf6N2E2_2586 at 3044.388 kb on + strand, within Pf6N2E2_2587 at 3044.388 kb on + strand, within Pf6N2E2_2587 at 3044.388 kb on + strand, within Pf6N2E2_2587 at 3044.443 kb on + strand, within Pf6N2E2_2587 at 3044.443 kb on + strand, within Pf6N2E2_2587 at 3044.444 kb on - strand, within Pf6N2E2_2587 at 3044.444 kb on - strand, within Pf6N2E2_2587 at 3044.485 kb on + strand, within Pf6N2E2_2587 at 3044.485 kb on + strand, within Pf6N2E2_2587 at 3044.485 kb on + strand, within Pf6N2E2_2587 at 3044.486 kb on - strand, within Pf6N2E2_2587 at 3044.486 kb on - strand, within Pf6N2E2_2587 at 3044.489 kb on - strand, within Pf6N2E2_2587 at 3044.489 kb on - strand, within Pf6N2E2_2587 at 3044.563 kb on + strand, within Pf6N2E2_2587 at 3044.564 kb on - strand, within Pf6N2E2_2587 at 3044.564 kb on - strand, within Pf6N2E2_2587 at 3044.564 kb on - strand, within Pf6N2E2_2587 at 3044.584 kb on + strand, within Pf6N2E2_2587 at 3044.585 kb on - strand, within Pf6N2E2_2587 at 3044.585 kb on - strand, within Pf6N2E2_2587 at 3044.625 kb on + strand, within Pf6N2E2_2587 at 3044.626 kb on - strand, within Pf6N2E2_2587 at 3044.626 kb on - strand, within Pf6N2E2_2587 at 3044.626 kb on - strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.641 kb on + strand, within Pf6N2E2_2587 at 3044.642 kb on - strand, within Pf6N2E2_2587
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 3,041,515 + Pf6N2E2_2583 0.88 +0.4 3,041,639 - -0.6 3,041,639 - -4.0 3,041,647 - +0.3 3,041,870 + Pf6N2E2_2584 0.46 +0.3 3,041,887 + Pf6N2E2_2584 0.50 +0.3 3,041,887 + Pf6N2E2_2584 0.50 -1.1 3,041,887 + Pf6N2E2_2584 0.50 +0.3 3,041,887 + Pf6N2E2_2584 0.50 -1.2 3,041,888 - Pf6N2E2_2584 0.50 +0.3 3,041,888 - Pf6N2E2_2584 0.50 -1.2 3,041,888 - Pf6N2E2_2584 0.50 +0.3 3,041,888 - Pf6N2E2_2584 0.50 +0.3 3,041,911 + Pf6N2E2_2584 0.54 +0.3 3,041,911 + Pf6N2E2_2584 0.54 +2.2 3,041,911 + Pf6N2E2_2584 0.54 +1.9 3,041,912 - Pf6N2E2_2584 0.55 -0.4 3,041,912 - Pf6N2E2_2584 0.55 -0.7 3,041,912 - Pf6N2E2_2584 0.55 -0.5 3,041,912 - Pf6N2E2_2584 0.55 -1.2 3,041,912 - Pf6N2E2_2584 0.55 +0.2 3,041,912 - Pf6N2E2_2584 0.55 -0.7 3,042,019 + Pf6N2E2_2584 0.76 +1.2 3,042,019 + Pf6N2E2_2584 0.76 -0.7 3,042,040 - Pf6N2E2_2584 0.80 -0.7 3,042,040 - Pf6N2E2_2584 0.80 +0.3 3,042,040 - Pf6N2E2_2584 0.80 -0.7 3,042,114 + +0.3 3,042,114 + -1.2 3,042,146 + +1.2 3,042,147 - +0.3 3,042,157 + +0.3 3,042,201 + -1.2 3,042,304 + +0.3 3,042,305 - +0.3 3,042,315 + -2.0 3,042,315 + +0.3 3,042,315 + -0.7 3,042,315 + +0.3 3,042,380 + +0.3 3,042,380 + +0.3 3,042,380 + -0.7 3,042,381 - -0.7 3,042,381 - +0.7 3,042,383 + +0.3 3,042,384 - +1.3 3,042,417 + +0.3 3,042,773 + Pf6N2E2_2585 0.29 +1.1 3,042,773 + Pf6N2E2_2585 0.29 +0.9 3,042,773 + Pf6N2E2_2585 0.29 +1.1 3,042,773 + Pf6N2E2_2585 0.29 +0.3 3,042,773 + Pf6N2E2_2585 0.29 +0.9 3,042,773 + Pf6N2E2_2585 0.29 -0.7 3,042,773 + Pf6N2E2_2585 0.29 +0.3 3,042,774 - Pf6N2E2_2585 0.29 +1.1 3,042,774 - Pf6N2E2_2585 0.29 +1.2 3,042,774 - Pf6N2E2_2585 0.29 +0.2 3,042,774 - Pf6N2E2_2585 0.29 +0.3 3,042,774 - Pf6N2E2_2585 0.29 +0.3 3,042,774 - Pf6N2E2_2585 0.29 +0.5 3,042,774 - Pf6N2E2_2585 0.29 +0.3 3,042,774 - Pf6N2E2_2585 0.29 +1.3 3,042,774 - Pf6N2E2_2585 0.29 +0.3 3,042,774 - Pf6N2E2_2585 0.29 +1.2 3,042,774 - Pf6N2E2_2585 0.29 +1.7 3,042,825 + Pf6N2E2_2585 0.33 +0.3 3,042,826 - Pf6N2E2_2585 0.33 -0.7 3,042,864 + Pf6N2E2_2585 0.36 +0.3 3,042,865 - Pf6N2E2_2585 0.36 -1.2 3,042,865 - Pf6N2E2_2585 0.36 -0.7 3,043,549 - Pf6N2E2_2585 0.86 +0.3 3,043,650 + +0.3 3,043,728 + -0.7 3,043,728 + +0.3 3,043,729 - +0.3 3,043,729 - +0.3 3,043,729 - +0.3 3,043,774 + -2.2 3,043,775 - -0.7 3,043,775 - +0.3 3,043,775 - +0.3 3,043,775 - +0.3 3,043,779 - -0.7 3,043,779 - +0.3 3,043,881 + Pf6N2E2_2586 0.13 +0.3 3,043,882 - Pf6N2E2_2586 0.14 +0.3 3,044,018 - Pf6N2E2_2586 0.53 -1.4 3,044,388 + Pf6N2E2_2587 0.10 +0.3 3,044,388 + Pf6N2E2_2587 0.10 -1.7 3,044,388 + Pf6N2E2_2587 0.10 -0.7 3,044,443 + Pf6N2E2_2587 0.14 +0.3 3,044,443 + Pf6N2E2_2587 0.14 -0.7 3,044,444 - Pf6N2E2_2587 0.14 +0.3 3,044,444 - Pf6N2E2_2587 0.14 -1.0 3,044,485 + Pf6N2E2_2587 0.16 +0.3 3,044,485 + Pf6N2E2_2587 0.16 -1.0 3,044,485 + Pf6N2E2_2587 0.16 +0.3 3,044,486 - Pf6N2E2_2587 0.16 -0.7 3,044,486 - Pf6N2E2_2587 0.16 +0.3 3,044,489 - Pf6N2E2_2587 0.16 +0.3 3,044,489 - Pf6N2E2_2587 0.16 -0.4 3,044,563 + Pf6N2E2_2587 0.21 -1.6 3,044,564 - Pf6N2E2_2587 0.21 +0.3 3,044,564 - Pf6N2E2_2587 0.21 +0.3 3,044,564 - Pf6N2E2_2587 0.21 +0.3 3,044,584 + Pf6N2E2_2587 0.22 -1.8 3,044,585 - Pf6N2E2_2587 0.22 +0.3 3,044,585 - Pf6N2E2_2587 0.22 +0.3 3,044,625 + Pf6N2E2_2587 0.24 +0.3 3,044,626 - Pf6N2E2_2587 0.24 +1.7 3,044,626 - Pf6N2E2_2587 0.24 -1.4 3,044,626 - Pf6N2E2_2587 0.24 +0.3 3,044,641 + Pf6N2E2_2587 0.25 -0.7 3,044,641 + Pf6N2E2_2587 0.25 -0.7 3,044,641 + Pf6N2E2_2587 0.25 -1.5 3,044,641 + Pf6N2E2_2587 0.25 -0.7 3,044,641 + Pf6N2E2_2587 0.25 -1.1 3,044,642 - Pf6N2E2_2587 0.25 +0.3
Or see this region's nucleotide sequence