Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_47

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_46 and Pf6N2E2_47 overlap by 4 nucleotidesPf6N2E2_47 and Pf6N2E2_48 are separated by 89 nucleotidesPf6N2E2_48 and Pf6N2E2_6095 are separated by 251 nucleotidesPf6N2E2_6095 and Pf6N2E2_49 are separated by 235 nucleotides Pf6N2E2_46 - membrane protein, putative, at 86,264 to 87,325 _46 Pf6N2E2_47 - FIG00961567: hypothetical protein, at 87,322 to 87,504 _47 Pf6N2E2_48 - FIG00955661: hypothetical protein, at 87,594 to 87,788 _48 Pf6N2E2_6095 - tRNA-Asn-GTT, at 88,040 to 88,112 _6095 Pf6N2E2_49 - FIG00967200: hypothetical protein, at 88,348 to 88,575 _49 Position (kb) 87 88Strain fitness (log2 ratio) -2 -1 0 1 2at 86.506 kb on + strand, within Pf6N2E2_46at 86.506 kb on + strand, within Pf6N2E2_46at 86.537 kb on + strand, within Pf6N2E2_46at 86.537 kb on + strand, within Pf6N2E2_46at 86.538 kb on - strand, within Pf6N2E2_46at 86.538 kb on - strand, within Pf6N2E2_46at 86.771 kb on + strand, within Pf6N2E2_46at 86.771 kb on + strand, within Pf6N2E2_46at 86.772 kb on - strand, within Pf6N2E2_46at 86.772 kb on - strand, within Pf6N2E2_46at 86.816 kb on - strand, within Pf6N2E2_46at 87.059 kb on + strand, within Pf6N2E2_46at 87.060 kb on - strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.185 kb on + strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.186 kb on - strand, within Pf6N2E2_46at 87.323 kb on + strandat 87.338 kb on + strandat 87.339 kb on - strandat 87.531 kb on + strandat 87.531 kb on + strandat 87.536 kb on + strandat 87.536 kb on + strandat 87.536 kb on + strandat 87.575 kb on + strandat 87.575 kb on + strandat 87.575 kb on + strandat 87.576 kb on - strandat 87.578 kb on + strandat 87.579 kb on - strandat 87.733 kb on + strand, within Pf6N2E2_48at 87.734 kb on - strand, within Pf6N2E2_48at 87.734 kb on - strand, within Pf6N2E2_48at 87.788 kb on + strandat 87.788 kb on + strandat 87.806 kb on + strandat 87.806 kb on + strandat 87.807 kb on - strandat 87.906 kb on + strandat 87.928 kb on + strandat 87.929 kb on - strandat 87.975 kb on + strandat 88.034 kb on - strandat 88.077 kb on - strand, within Pf6N2E2_6095at 88.077 kb on - strand, within Pf6N2E2_6095at 88.093 kb on - strand, within Pf6N2E2_6095at 88.093 kb on - strand, within Pf6N2E2_6095at 88.126 kb on + strandat 88.233 kb on + strandat 88.234 kb on - strandat 88.285 kb on + strandat 88.286 kb on - strandat 88.286 kb on - strandat 88.286 kb on - strandat 88.286 kb on - strandat 88.291 kb on - strandat 88.291 kb on - strandat 88.311 kb on + strandat 88.312 kb on - strandat 88.405 kb on + strand, within Pf6N2E2_49at 88.406 kb on - strand, within Pf6N2E2_49at 88.406 kb on - strand, within Pf6N2E2_49at 88.491 kb on + strand, within Pf6N2E2_49

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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86,506 + Pf6N2E2_46 0.23 +0.3
86,506 + Pf6N2E2_46 0.23 -0.4
86,537 + Pf6N2E2_46 0.26 +0.3
86,537 + Pf6N2E2_46 0.26 +0.3
86,538 - Pf6N2E2_46 0.26 +0.3
86,538 - Pf6N2E2_46 0.26 +0.3
86,771 + Pf6N2E2_46 0.48 -0.7
86,771 + Pf6N2E2_46 0.48 +0.3
86,772 - Pf6N2E2_46 0.48 -0.0
86,772 - Pf6N2E2_46 0.48 +0.3
86,816 - Pf6N2E2_46 0.52 +0.3
87,059 + Pf6N2E2_46 0.75 -0.6
87,060 - Pf6N2E2_46 0.75 +0.3
87,185 + Pf6N2E2_46 0.87 +0.3
87,185 + Pf6N2E2_46 0.87 +0.3
87,185 + Pf6N2E2_46 0.87 -0.7
87,185 + Pf6N2E2_46 0.87 -0.4
87,185 + Pf6N2E2_46 0.87 +0.3
87,186 - Pf6N2E2_46 0.87 -0.4
87,186 - Pf6N2E2_46 0.87 -0.5
87,186 - Pf6N2E2_46 0.87 -0.7
87,323 + -0.7
87,338 + +0.3
87,339 - +0.3
87,531 + +0.3
87,531 + +0.3
87,536 + +0.3
87,536 + +0.3
87,536 + +0.3
87,575 + +0.0
87,575 + -0.7
87,575 + +0.3
87,576 - -0.7
87,578 + -0.7
87,579 - -0.3
87,733 + Pf6N2E2_48 0.71 -0.7
87,734 - Pf6N2E2_48 0.72 -0.7
87,734 - Pf6N2E2_48 0.72 -1.3
87,788 + +1.9
87,788 + -1.3
87,806 + +0.3
87,806 + -0.0
87,807 - +0.3
87,906 + +0.3
87,928 + -1.3
87,929 - -0.7
87,975 + +0.3
88,034 - +1.0
88,077 - Pf6N2E2_6095 0.51 -0.7
88,077 - Pf6N2E2_6095 0.51 -0.3
88,093 - Pf6N2E2_6095 0.73 -0.7
88,093 - Pf6N2E2_6095 0.73 +0.5
88,126 + +0.3
88,233 + -0.7
88,234 - -0.4
88,285 + -0.4
88,286 - -0.5
88,286 - +0.3
88,286 - +0.3
88,286 - -0.1
88,291 - +0.3
88,291 - +0.3
88,311 + -0.7
88,312 - -0.7
88,405 + Pf6N2E2_49 0.25 +0.3
88,406 - Pf6N2E2_49 0.25 +0.3
88,406 - Pf6N2E2_49 0.25 -2.0
88,491 + Pf6N2E2_49 0.63 +0.3

Or see this region's nucleotide sequence