Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1033

Experiment: Ying_all64 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1031 and Pf6N2E2_1032 are separated by 1 nucleotidesPf6N2E2_1032 and Pf6N2E2_1033 are separated by 25 nucleotidesPf6N2E2_1033 and Pf6N2E2_1034 are separated by 46 nucleotidesPf6N2E2_1034 and Pf6N2E2_1035 are separated by 84 nucleotides Pf6N2E2_1031 - Alkanesulfonates transport system permease protein, at 1,205,278 to 1,206,222 _1031 Pf6N2E2_1032 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 1,206,224 to 1,207,036 _1032 Pf6N2E2_1033 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,207,062 to 1,208,087 _1033 Pf6N2E2_1034 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,208,134 to 1,208,943 _1034 Pf6N2E2_1035 - MotA/TolQ/ExbB proton channel family protein, at 1,209,028 to 1,209,753 _1035 Position (kb) 1207 1208 1209Strain fitness (log2 ratio) -3 -2 -1 0 1at 1206.133 kb on + strandat 1206.133 kb on + strandat 1206.133 kb on + strandat 1206.133 kb on + strandat 1206.134 kb on - strandat 1206.134 kb on - strandat 1206.134 kb on - strandat 1206.134 kb on - strandat 1206.134 kb on - strandat 1206.224 kb on - strandat 1206.757 kb on - strand, within Pf6N2E2_1032at 1207.000 kb on + strandat 1207.006 kb on + strandat 1207.006 kb on + strandat 1207.007 kb on - strandat 1207.007 kb on - strandat 1207.035 kb on - strandat 1207.035 kb on - strandat 1207.263 kb on + strand, within Pf6N2E2_1033at 1207.264 kb on - strand, within Pf6N2E2_1033at 1207.264 kb on - strand, within Pf6N2E2_1033at 1207.528 kb on - strand, within Pf6N2E2_1033at 1207.846 kb on - strand, within Pf6N2E2_1033at 1207.927 kb on + strand, within Pf6N2E2_1033at 1208.119 kb on + strandat 1208.120 kb on - strandat 1208.120 kb on - strandat 1208.395 kb on - strand, within Pf6N2E2_1034at 1208.689 kb on + strand, within Pf6N2E2_1034at 1208.942 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 3
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1,206,133 + -0.3
1,206,133 + -0.1
1,206,133 + +0.1
1,206,133 + +0.1
1,206,134 - -0.9
1,206,134 - +0.1
1,206,134 - +0.1
1,206,134 - +0.1
1,206,134 - +0.1
1,206,224 - +0.1
1,206,757 - Pf6N2E2_1032 0.66 -0.4
1,207,000 + +0.1
1,207,006 + +0.1
1,207,006 + +0.1
1,207,007 - +0.7
1,207,007 - -1.4
1,207,035 - +0.1
1,207,035 - +0.0
1,207,263 + Pf6N2E2_1033 0.20 -0.9
1,207,264 - Pf6N2E2_1033 0.20 -1.4
1,207,264 - Pf6N2E2_1033 0.20 +0.1
1,207,528 - Pf6N2E2_1033 0.45 +0.1
1,207,846 - Pf6N2E2_1033 0.76 -3.0
1,207,927 + Pf6N2E2_1033 0.84 +0.1
1,208,119 + -0.5
1,208,120 - +0.1
1,208,120 - -0.9
1,208,395 - Pf6N2E2_1034 0.32 -0.6
1,208,689 + Pf6N2E2_1034 0.69 +1.7
1,208,942 - +0.1

Or see this region's nucleotide sequence