Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_650

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_649 and Pf6N2E2_650 are separated by 16 nucleotidesPf6N2E2_650 and Pf6N2E2_651 overlap by 4 nucleotides Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835 _649 Pf6N2E2_650 - General secretion pathway protein M, at 738,852 to 739,400 _650 Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760 _651 Position (kb) 738 739 740Strain fitness (log2 ratio) -1 0 1at 738.077 kb on + strand, within Pf6N2E2_649at 738.113 kb on - strand, within Pf6N2E2_649at 738.413 kb on + strand, within Pf6N2E2_649at 738.834 kb on - strandat 738.834 kb on - strandat 739.008 kb on + strand, within Pf6N2E2_650at 739.152 kb on + strand, within Pf6N2E2_650at 739.152 kb on + strand, within Pf6N2E2_650at 739.153 kb on - strand, within Pf6N2E2_650at 739.153 kb on - strand, within Pf6N2E2_650at 739.153 kb on - strand, within Pf6N2E2_650at 739.156 kb on + strand, within Pf6N2E2_650at 739.157 kb on - strand, within Pf6N2E2_650at 739.597 kb on + strandat 739.946 kb on + strand, within Pf6N2E2_651at 739.947 kb on - strand, within Pf6N2E2_651at 740.004 kb on - strand, within Pf6N2E2_651at 740.354 kb on + strand, within Pf6N2E2_651at 740.354 kb on + strand, within Pf6N2E2_651at 740.354 kb on + strand, within Pf6N2E2_651

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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738,077 + Pf6N2E2_649 0.35 -0.8
738,113 - Pf6N2E2_649 0.38 +0.2
738,413 + Pf6N2E2_649 0.64 -0.8
738,834 - -0.8
738,834 - +0.2
739,008 + Pf6N2E2_650 0.28 -0.8
739,152 + Pf6N2E2_650 0.55 +0.2
739,152 + Pf6N2E2_650 0.55 -0.8
739,153 - Pf6N2E2_650 0.55 +0.2
739,153 - Pf6N2E2_650 0.55 -0.8
739,153 - Pf6N2E2_650 0.55 -0.8
739,156 + Pf6N2E2_650 0.55 -1.1
739,157 - Pf6N2E2_650 0.56 -0.8
739,597 + -0.3
739,946 + Pf6N2E2_651 0.23 +0.2
739,947 - Pf6N2E2_651 0.23 +0.2
740,004 - Pf6N2E2_651 0.26 -0.9
740,354 + Pf6N2E2_651 0.40 +0.2
740,354 + Pf6N2E2_651 0.40 -0.8
740,354 + Pf6N2E2_651 0.40 -1.4

Or see this region's nucleotide sequence