Experiment: Ying_all64 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4757 and Pf6N2E2_4758 are separated by 58 nucleotides Pf6N2E2_4758 and Pf6N2E2_4759 are separated by 91 nucleotides Pf6N2E2_4759 and Pf6N2E2_4760 overlap by 19 nucleotides Pf6N2E2_4760 and Pf6N2E2_4761 are separated by 36 nucleotides
Pf6N2E2_4757 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 5,496,502 to 5,497,266
_4757
Pf6N2E2_4758 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 5,497,325 to 5,498,065
_4758
Pf6N2E2_4759 - Biotin synthase (EC 2.8.1.6), at 5,498,157 to 5,499,212
_4759
Pf6N2E2_4760 - hypothetical protein, at 5,499,194 to 5,499,337
_4760
Pf6N2E2_4761 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 5,499,374 to 5,500,552
_4761
Position (kb) 5498
5499
5500 Strain fitness (log2 ratio) -2
-1
0
1 at 5497.365 kb on + strand at 5497.365 kb on + strand at 5497.365 kb on + strand at 5497.365 kb on + strand at 5497.366 kb on - strand at 5497.366 kb on - strand at 5497.366 kb on - strand at 5497.366 kb on - strand at 5497.821 kb on + strand, within Pf6N2E2_4758 at 5497.822 kb on - strand, within Pf6N2E2_4758 at 5497.822 kb on - strand, within Pf6N2E2_4758 at 5497.966 kb on + strand, within Pf6N2E2_4758 at 5497.966 kb on + strand, within Pf6N2E2_4758 at 5497.966 kb on + strand, within Pf6N2E2_4758 at 5497.967 kb on - strand, within Pf6N2E2_4758 at 5497.967 kb on - strand, within Pf6N2E2_4758 at 5497.967 kb on - strand, within Pf6N2E2_4758 at 5497.967 kb on - strand, within Pf6N2E2_4758 at 5498.076 kb on + strand at 5498.076 kb on + strand at 5498.076 kb on + strand at 5498.076 kb on + strand at 5498.076 kb on + strand at 5498.077 kb on - strand at 5498.077 kb on - strand at 5498.077 kb on - strand at 5498.080 kb on + strand at 5498.080 kb on + strand at 5498.080 kb on + strand at 5498.080 kb on + strand at 5498.081 kb on - strand at 5498.140 kb on + strand at 5498.140 kb on + strand at 5498.140 kb on + strand at 5498.141 kb on - strand at 5498.141 kb on - strand at 5498.141 kb on - strand at 5498.141 kb on - strand at 5498.141 kb on - strand at 5498.141 kb on - strand at 5498.141 kb on - strand at 5498.349 kb on + strand, within Pf6N2E2_4759 at 5498.349 kb on + strand, within Pf6N2E2_4759 at 5498.349 kb on + strand, within Pf6N2E2_4759 at 5498.349 kb on + strand, within Pf6N2E2_4759 at 5498.349 kb on + strand, within Pf6N2E2_4759 at 5498.350 kb on - strand, within Pf6N2E2_4759 at 5498.350 kb on - strand, within Pf6N2E2_4759 at 5498.350 kb on - strand, within Pf6N2E2_4759 at 5498.350 kb on - strand, within Pf6N2E2_4759 at 5498.350 kb on - strand, within Pf6N2E2_4759 at 5498.351 kb on - strand, within Pf6N2E2_4759 at 5498.370 kb on + strand, within Pf6N2E2_4759 at 5498.370 kb on + strand, within Pf6N2E2_4759 at 5498.370 kb on + strand, within Pf6N2E2_4759 at 5498.370 kb on + strand, within Pf6N2E2_4759 at 5498.370 kb on + strand, within Pf6N2E2_4759 at 5498.371 kb on - strand, within Pf6N2E2_4759 at 5498.371 kb on - strand, within Pf6N2E2_4759 at 5498.371 kb on - strand, within Pf6N2E2_4759 at 5498.371 kb on - strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.509 kb on - strand, within Pf6N2E2_4759 at 5498.509 kb on - strand, within Pf6N2E2_4759 at 5498.509 kb on - strand, within Pf6N2E2_4759 at 5498.619 kb on + strand, within Pf6N2E2_4759 at 5498.619 kb on + strand, within Pf6N2E2_4759 at 5498.620 kb on - strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.630 kb on + strand, within Pf6N2E2_4759 at 5498.630 kb on + strand, within Pf6N2E2_4759 at 5498.631 kb on - strand, within Pf6N2E2_4759 at 5498.631 kb on - strand, within Pf6N2E2_4759 at 5498.631 kb on - strand, within Pf6N2E2_4759 at 5498.656 kb on - strand, within Pf6N2E2_4759 at 5498.656 kb on - strand, within Pf6N2E2_4759 at 5498.683 kb on - strand, within Pf6N2E2_4759 at 5498.683 kb on - strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.710 kb on - strand, within Pf6N2E2_4759 at 5498.710 kb on - strand, within Pf6N2E2_4759 at 5498.710 kb on - strand, within Pf6N2E2_4759 at 5499.195 kb on + strand at 5499.196 kb on - strand at 5499.196 kb on - strand at 5499.356 kb on + strand at 5499.426 kb on - strand at 5499.513 kb on - strand, within Pf6N2E2_4761 at 5499.513 kb on - strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.693 kb on - strand, within Pf6N2E2_4761 at 5499.816 kb on - strand, within Pf6N2E2_4761 at 5499.872 kb on - strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.158 kb on - strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3 remove 5,497,365 + +0.5 5,497,365 + +0.5 5,497,365 + -1.1 5,497,365 + +0.5 5,497,366 - +1.5 5,497,366 - +0.5 5,497,366 - +0.5 5,497,366 - +0.5 5,497,821 + Pf6N2E2_4758 0.67 -0.1 5,497,822 - Pf6N2E2_4758 0.67 -0.6 5,497,822 - Pf6N2E2_4758 0.67 -0.5 5,497,966 + Pf6N2E2_4758 0.87 -0.5 5,497,966 + Pf6N2E2_4758 0.87 -1.7 5,497,966 + Pf6N2E2_4758 0.87 -0.5 5,497,967 - Pf6N2E2_4758 0.87 -0.6 5,497,967 - Pf6N2E2_4758 0.87 +0.5 5,497,967 - Pf6N2E2_4758 0.87 -0.2 5,497,967 - Pf6N2E2_4758 0.87 -0.5 5,498,076 + -0.7 5,498,076 + +0.5 5,498,076 + +0.5 5,498,076 + +0.5 5,498,076 + +0.5 5,498,077 - -1.1 5,498,077 - -0.5 5,498,077 - -1.1 5,498,080 + +0.5 5,498,080 + -1.1 5,498,080 + -1.2 5,498,080 + -0.5 5,498,081 - +0.5 5,498,140 + -1.1 5,498,140 + +0.5 5,498,140 + -0.5 5,498,141 - +0.5 5,498,141 - +0.5 5,498,141 - -0.5 5,498,141 - +0.5 5,498,141 - +0.5 5,498,141 - +0.5 5,498,141 - -0.5 5,498,349 + Pf6N2E2_4759 0.18 +0.5 5,498,349 + Pf6N2E2_4759 0.18 -0.5 5,498,349 + Pf6N2E2_4759 0.18 -1.1 5,498,349 + Pf6N2E2_4759 0.18 -1.8 5,498,349 + Pf6N2E2_4759 0.18 +0.5 5,498,350 - Pf6N2E2_4759 0.18 -0.5 5,498,350 - Pf6N2E2_4759 0.18 +0.5 5,498,350 - Pf6N2E2_4759 0.18 -1.1 5,498,350 - Pf6N2E2_4759 0.18 +0.5 5,498,350 - Pf6N2E2_4759 0.18 -0.5 5,498,351 - Pf6N2E2_4759 0.18 -0.5 5,498,370 + Pf6N2E2_4759 0.20 +0.5 5,498,370 + Pf6N2E2_4759 0.20 -0.5 5,498,370 + Pf6N2E2_4759 0.20 +0.5 5,498,370 + Pf6N2E2_4759 0.20 +0.5 5,498,370 + Pf6N2E2_4759 0.20 -0.5 5,498,371 - Pf6N2E2_4759 0.20 -0.5 5,498,371 - Pf6N2E2_4759 0.20 +0.5 5,498,371 - Pf6N2E2_4759 0.20 +0.5 5,498,371 - Pf6N2E2_4759 0.20 +0.5 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.5 5,498,508 + Pf6N2E2_4759 0.33 -1.1 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.8 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.5 5,498,508 + Pf6N2E2_4759 0.33 -0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.1 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -0.5 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,509 - Pf6N2E2_4759 0.33 -0.5 5,498,509 - Pf6N2E2_4759 0.33 +0.5 5,498,509 - Pf6N2E2_4759 0.33 +0.5 5,498,619 + Pf6N2E2_4759 0.44 +0.5 5,498,619 + Pf6N2E2_4759 0.44 -0.5 5,498,620 - Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -1.1 5,498,622 + Pf6N2E2_4759 0.44 -0.1 5,498,622 + Pf6N2E2_4759 0.44 -1.8 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 +0.5 5,498,622 + Pf6N2E2_4759 0.44 +0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -1.8 5,498,622 + Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -1.1 5,498,623 - Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -0.5 5,498,623 - Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -0.5 5,498,623 - Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -0.5 5,498,630 + Pf6N2E2_4759 0.45 +0.5 5,498,630 + Pf6N2E2_4759 0.45 -0.5 5,498,631 - Pf6N2E2_4759 0.45 +0.5 5,498,631 - Pf6N2E2_4759 0.45 -0.5 5,498,631 - Pf6N2E2_4759 0.45 +0.5 5,498,656 - Pf6N2E2_4759 0.47 +0.5 5,498,656 - Pf6N2E2_4759 0.47 -0.5 5,498,683 - Pf6N2E2_4759 0.50 -1.1 5,498,683 - Pf6N2E2_4759 0.50 +0.5 5,498,709 + Pf6N2E2_4759 0.52 +0.5 5,498,709 + Pf6N2E2_4759 0.52 +0.5 5,498,709 + Pf6N2E2_4759 0.52 -0.5 5,498,709 + Pf6N2E2_4759 0.52 -1.1 5,498,709 + Pf6N2E2_4759 0.52 -1.5 5,498,709 + Pf6N2E2_4759 0.52 -1.1 5,498,710 - Pf6N2E2_4759 0.52 +0.5 5,498,710 - Pf6N2E2_4759 0.52 +0.5 5,498,710 - Pf6N2E2_4759 0.52 +0.5 5,499,195 + +0.5 5,499,196 - -0.5 5,499,196 - +0.5 5,499,356 + -1.1 5,499,426 - -1.5 5,499,513 - Pf6N2E2_4761 0.12 -0.5 5,499,513 - Pf6N2E2_4761 0.12 -0.5 5,499,692 + Pf6N2E2_4761 0.27 +0.5 5,499,692 + Pf6N2E2_4761 0.27 +0.5 5,499,692 + Pf6N2E2_4761 0.27 -0.5 5,499,692 + Pf6N2E2_4761 0.27 +0.5 5,499,693 - Pf6N2E2_4761 0.27 +0.5 5,499,816 - Pf6N2E2_4761 0.37 +0.5 5,499,872 - Pf6N2E2_4761 0.42 +0.5 5,500,157 + Pf6N2E2_4761 0.66 -1.1 5,500,157 + Pf6N2E2_4761 0.66 -0.5 5,500,157 + Pf6N2E2_4761 0.66 -0.5 5,500,157 + Pf6N2E2_4761 0.66 -1.1 5,500,157 + Pf6N2E2_4761 0.66 -0.5 5,500,158 - Pf6N2E2_4761 0.66 +0.5 5,500,163 + Pf6N2E2_4761 0.67 +0.5 5,500,163 + Pf6N2E2_4761 0.67 -0.5 5,500,163 + Pf6N2E2_4761 0.67 +0.5 5,500,163 + Pf6N2E2_4761 0.67 -0.5 5,500,163 + Pf6N2E2_4761 0.67 -0.5 5,500,163 + Pf6N2E2_4761 0.67 +1.5 5,500,163 + Pf6N2E2_4761 0.67 -1.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 -1.1 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,164 - Pf6N2E2_4761 0.67 -0.5
Or see this region's nucleotide sequence