Experiment: Ying_all64 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3276 and Pf6N2E2_3277 overlap by 8 nucleotides Pf6N2E2_3277 and Pf6N2E2_3278 are separated by 284 nucleotides
Pf6N2E2_3276 - Fumarate hydratase class II (EC 4.2.1.2), at 3,779,056 to 3,780,432
_3276
Pf6N2E2_3277 - FagA protein, at 3,780,425 to 3,780,820
_3277
Pf6N2E2_3278 - TldE protein, part of TldE/TldD proteolytic complex, at 3,781,105 to 3,782,463
_3278
Position (kb)
3780
3781 Strain fitness (log2 ratio)
-2
-1
0
1 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3780.194 kb on - strand, within Pf6N2E2_3276 at 3780.253 kb on + strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.315 kb on + strand at 3780.315 kb on + strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.457 kb on + strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.715 kb on - strand, within Pf6N2E2_3277 at 3780.792 kb on + strand at 3780.792 kb on + strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.950 kb on + strand at 3781.189 kb on + strand at 3781.216 kb on - strand at 3781.295 kb on + strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.410 kb on + strand, within Pf6N2E2_3278 at 3781.411 kb on - strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.463 kb on + strand, within Pf6N2E2_3278 at 3781.464 kb on - strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.478 kb on + strand, within Pf6N2E2_3278 at 3781.479 kb on - strand, within Pf6N2E2_3278 at 3781.479 kb on - strand, within Pf6N2E2_3278 at 3781.517 kb on + strand, within Pf6N2E2_3278 at 3781.517 kb on + strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278 at 3781.518 kb on - strand, within Pf6N2E2_3278
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3 remove 3,779,950 + Pf6N2E2_3276 0.65 +0.3 3,779,950 + Pf6N2E2_3276 0.65 +0.4 3,779,950 + Pf6N2E2_3276 0.65 -1.4 3,779,951 - Pf6N2E2_3276 0.65 -0.6 3,779,951 - Pf6N2E2_3276 0.65 +0.4 3,780,194 - Pf6N2E2_3276 0.83 +0.4 3,780,253 + Pf6N2E2_3276 0.87 -1.1 3,780,254 - Pf6N2E2_3276 0.87 -1.7 3,780,254 - Pf6N2E2_3276 0.87 -1.1 3,780,254 - Pf6N2E2_3276 0.87 -0.6 3,780,315 + -0.1 3,780,315 + -0.6 3,780,316 - +0.4 3,780,316 - +0.4 3,780,316 - -0.6 3,780,424 - -1.1 3,780,424 - -0.6 3,780,424 - +0.4 3,780,426 + +0.4 3,780,426 + +0.4 3,780,426 + -1.1 3,780,426 + -0.6 3,780,427 - -0.7 3,780,427 - -1.6 3,780,427 - +0.4 3,780,427 - -1.1 3,780,427 - +0.4 3,780,457 + +0.1 3,780,458 - -1.1 3,780,458 - -1.1 3,780,458 - -2.1 3,780,714 + Pf6N2E2_3277 0.73 +0.4 3,780,714 + Pf6N2E2_3277 0.73 -1.9 3,780,715 - Pf6N2E2_3277 0.73 -0.6 3,780,792 + -1.1 3,780,792 + -0.6 3,780,793 - +0.4 3,780,793 - -0.9 3,780,793 - -2.1 3,780,950 + +0.0 3,781,189 + -1.4 3,781,216 - -0.1 3,781,295 + Pf6N2E2_3278 0.14 -0.6 3,781,296 - Pf6N2E2_3278 0.14 -0.6 3,781,296 - Pf6N2E2_3278 0.14 -1.9 3,781,410 + Pf6N2E2_3278 0.22 -0.6 3,781,411 - Pf6N2E2_3278 0.23 +0.4 3,781,463 + Pf6N2E2_3278 0.26 +0.4 3,781,463 + Pf6N2E2_3278 0.26 -1.1 3,781,463 + Pf6N2E2_3278 0.26 -1.3 3,781,464 - Pf6N2E2_3278 0.26 -0.6 3,781,478 + Pf6N2E2_3278 0.27 +0.4 3,781,478 + Pf6N2E2_3278 0.27 +1.2 3,781,478 + Pf6N2E2_3278 0.27 +0.4 3,781,478 + Pf6N2E2_3278 0.27 +0.4 3,781,478 + Pf6N2E2_3278 0.27 +0.4 3,781,478 + Pf6N2E2_3278 0.27 +0.4 3,781,479 - Pf6N2E2_3278 0.28 -1.6 3,781,479 - Pf6N2E2_3278 0.28 +0.4 3,781,517 + Pf6N2E2_3278 0.30 +0.4 3,781,517 + Pf6N2E2_3278 0.30 -0.6 3,781,518 - Pf6N2E2_3278 0.30 +0.4 3,781,518 - Pf6N2E2_3278 0.30 +0.4 3,781,518 - Pf6N2E2_3278 0.30 -1.1
Or see this region's nucleotide sequence