Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2927

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2925 and Pf6N2E2_2926 are separated by 0 nucleotidesPf6N2E2_2926 and Pf6N2E2_2927 are separated by 277 nucleotidesPf6N2E2_2927 and Pf6N2E2_2928 are separated by 27 nucleotidesPf6N2E2_2928 and Pf6N2E2_2929 overlap by 4 nucleotides Pf6N2E2_2925 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 1 LivG (from data), at 3,418,426 to 3,419,193 _2925 Pf6N2E2_2926 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data), at 3,419,194 to 3,419,895 _2926 Pf6N2E2_2927 - Putative signal peptide protein, at 3,420,173 to 3,420,478 _2927 Pf6N2E2_2928 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,420,506 to 3,421,375 _2928 Pf6N2E2_2929 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,421,372 to 3,422,130 _2929 Position (kb) 3420 3421Strain fitness (log2 ratio) -3 -2 -1 0 1at 3419.191 kb on + strandat 3419.191 kb on + strandat 3419.191 kb on + strandat 3419.191 kb on + strandat 3419.192 kb on - strandat 3419.225 kb on - strandat 3419.371 kb on + strand, within Pf6N2E2_2926at 3419.371 kb on + strand, within Pf6N2E2_2926at 3419.453 kb on + strand, within Pf6N2E2_2926at 3419.453 kb on + strand, within Pf6N2E2_2926at 3419.454 kb on - strand, within Pf6N2E2_2926at 3419.518 kb on + strand, within Pf6N2E2_2926at 3419.519 kb on - strand, within Pf6N2E2_2926at 3419.804 kb on - strand, within Pf6N2E2_2926at 3419.804 kb on - strand, within Pf6N2E2_2926at 3419.804 kb on - strand, within Pf6N2E2_2926at 3419.882 kb on - strandat 3420.011 kb on - strandat 3420.091 kb on + strandat 3420.115 kb on + strandat 3420.115 kb on + strandat 3420.116 kb on - strandat 3420.116 kb on - strandat 3420.116 kb on - strandat 3420.116 kb on - strandat 3420.368 kb on + strand, within Pf6N2E2_2927at 3420.368 kb on + strand, within Pf6N2E2_2927at 3420.369 kb on - strand, within Pf6N2E2_2927at 3420.514 kb on + strandat 3420.591 kb on + strandat 3420.592 kb on - strandat 3420.592 kb on - strandat 3420.641 kb on + strand, within Pf6N2E2_2928at 3420.641 kb on + strand, within Pf6N2E2_2928at 3420.641 kb on + strand, within Pf6N2E2_2928at 3420.642 kb on - strand, within Pf6N2E2_2928at 3420.642 kb on - strand, within Pf6N2E2_2928at 3420.642 kb on - strand, within Pf6N2E2_2928at 3420.642 kb on - strand, within Pf6N2E2_2928at 3420.642 kb on - strand, within Pf6N2E2_2928at 3420.872 kb on + strand, within Pf6N2E2_2928at 3420.873 kb on - strand, within Pf6N2E2_2928at 3420.938 kb on - strand, within Pf6N2E2_2928at 3420.938 kb on - strand, within Pf6N2E2_2928at 3420.965 kb on + strand, within Pf6N2E2_2928at 3420.965 kb on + strand, within Pf6N2E2_2928at 3420.966 kb on - strand, within Pf6N2E2_2928at 3420.966 kb on - strand, within Pf6N2E2_2928at 3421.064 kb on + strand, within Pf6N2E2_2928at 3421.064 kb on + strand, within Pf6N2E2_2928at 3421.065 kb on - strand, within Pf6N2E2_2928at 3421.194 kb on + strand, within Pf6N2E2_2928at 3421.238 kb on + strand, within Pf6N2E2_2928at 3421.239 kb on - strand, within Pf6N2E2_2928at 3421.239 kb on - strand, within Pf6N2E2_2928at 3421.239 kb on - strand, within Pf6N2E2_2928at 3421.239 kb on - strand, within Pf6N2E2_2928at 3421.239 kb on - strand, within Pf6N2E2_2928at 3421.239 kb on - strand, within Pf6N2E2_2928

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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3,419,191 + +0.5
3,419,191 + +0.4
3,419,191 + +0.4
3,419,191 + +0.4
3,419,192 - +0.4
3,419,225 - +0.4
3,419,371 + Pf6N2E2_2926 0.25 -0.4
3,419,371 + Pf6N2E2_2926 0.25 +0.4
3,419,453 + Pf6N2E2_2926 0.37 -0.6
3,419,453 + Pf6N2E2_2926 0.37 -0.6
3,419,454 - Pf6N2E2_2926 0.37 +0.4
3,419,518 + Pf6N2E2_2926 0.46 -0.7
3,419,519 - Pf6N2E2_2926 0.46 -1.2
3,419,804 - Pf6N2E2_2926 0.87 +0.4
3,419,804 - Pf6N2E2_2926 0.87 -0.4
3,419,804 - Pf6N2E2_2926 0.87 -0.6
3,419,882 - -0.6
3,420,011 - -0.3
3,420,091 + -0.6
3,420,115 + -3.4
3,420,115 + -0.6
3,420,116 - -0.5
3,420,116 - -1.2
3,420,116 - -1.2
3,420,116 - +0.4
3,420,368 + Pf6N2E2_2927 0.64 -1.5
3,420,368 + Pf6N2E2_2927 0.64 -0.6
3,420,369 - Pf6N2E2_2927 0.64 -1.6
3,420,514 + -0.6
3,420,591 + -1.6
3,420,592 - +0.4
3,420,592 - -1.8
3,420,641 + Pf6N2E2_2928 0.16 +0.4
3,420,641 + Pf6N2E2_2928 0.16 +0.4
3,420,641 + Pf6N2E2_2928 0.16 -1.9
3,420,642 - Pf6N2E2_2928 0.16 +0.4
3,420,642 - Pf6N2E2_2928 0.16 -0.6
3,420,642 - Pf6N2E2_2928 0.16 +0.4
3,420,642 - Pf6N2E2_2928 0.16 -0.0
3,420,642 - Pf6N2E2_2928 0.16 -0.9
3,420,872 + Pf6N2E2_2928 0.42 +0.4
3,420,873 - Pf6N2E2_2928 0.42 +0.4
3,420,938 - Pf6N2E2_2928 0.50 -1.2
3,420,938 - Pf6N2E2_2928 0.50 +0.4
3,420,965 + Pf6N2E2_2928 0.53 -1.2
3,420,965 + Pf6N2E2_2928 0.53 -0.5
3,420,966 - Pf6N2E2_2928 0.53 -1.6
3,420,966 - Pf6N2E2_2928 0.53 +0.4
3,421,064 + Pf6N2E2_2928 0.64 +0.4
3,421,064 + Pf6N2E2_2928 0.64 +0.4
3,421,065 - Pf6N2E2_2928 0.64 +0.4
3,421,194 + Pf6N2E2_2928 0.79 -0.3
3,421,238 + Pf6N2E2_2928 0.84 +0.3
3,421,239 - Pf6N2E2_2928 0.84 -0.6
3,421,239 - Pf6N2E2_2928 0.84 +0.4
3,421,239 - Pf6N2E2_2928 0.84 -1.2
3,421,239 - Pf6N2E2_2928 0.84 +0.4
3,421,239 - Pf6N2E2_2928 0.84 -0.3
3,421,239 - Pf6N2E2_2928 0.84 -0.0

Or see this region's nucleotide sequence