Experiment: Ying_all64 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1959 and Pf6N2E2_1960 are separated by 57 nucleotides Pf6N2E2_1960 and Pf6N2E2_1961 are separated by 57 nucleotides Pf6N2E2_1961 and Pf6N2E2_1962 are separated by 10 nucleotides
Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,323,967 to 2,324,740
_1959
Pf6N2E2_1960 - ABC transporter for D-sorbitol, ATPase component (from data) , at 2,324,798 to 2,325,895
_1960
Pf6N2E2_1961 - ABC transporter for D-sorbitol, permease component 2 (from data) , at 2,325,953 to 2,326,786
_1961
Pf6N2E2_1962 - ABC transporter for D-sorbitol, permease component 1 (from data) , at 2,326,797 to 2,327,639
_1962
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2323.800 kb on + strand at 2323.804 kb on + strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.824 kb on + strand at 2323.999 kb on - strand at 2324.016 kb on + strand at 2324.123 kb on + strand, within Pf6N2E2_1959 at 2324.251 kb on + strand, within Pf6N2E2_1959 at 2324.578 kb on - strand, within Pf6N2E2_1959 at 2324.637 kb on - strand, within Pf6N2E2_1959 at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.722 kb on + strand at 2324.748 kb on - strand at 2324.881 kb on - strand at 2324.881 kb on - strand at 2325.232 kb on - strand, within Pf6N2E2_1960 at 2325.232 kb on - strand, within Pf6N2E2_1960 at 2325.330 kb on + strand, within Pf6N2E2_1960 at 2325.331 kb on - strand, within Pf6N2E2_1960 at 2325.331 kb on - strand, within Pf6N2E2_1960 at 2325.355 kb on + strand, within Pf6N2E2_1960 at 2325.387 kb on - strand, within Pf6N2E2_1960 at 2325.502 kb on + strand, within Pf6N2E2_1960 at 2325.502 kb on + strand, within Pf6N2E2_1960 at 2325.503 kb on - strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.900 kb on - strand at 2326.021 kb on + strand at 2326.021 kb on + strand at 2326.021 kb on + strand at 2326.022 kb on - strand at 2326.022 kb on - strand at 2326.022 kb on - strand at 2326.299 kb on + strand, within Pf6N2E2_1961 at 2326.306 kb on - strand, within Pf6N2E2_1961 at 2326.345 kb on - strand, within Pf6N2E2_1961 at 2326.345 kb on - strand, within Pf6N2E2_1961 at 2326.345 kb on - strand, within Pf6N2E2_1961 at 2326.401 kb on + strand, within Pf6N2E2_1961 at 2326.401 kb on + strand, within Pf6N2E2_1961 at 2326.401 kb on + strand, within Pf6N2E2_1961 at 2326.401 kb on + strand, within Pf6N2E2_1961 at 2326.401 kb on + strand, within Pf6N2E2_1961 at 2326.401 kb on + strand, within Pf6N2E2_1961 at 2326.405 kb on + strand, within Pf6N2E2_1961 at 2326.406 kb on - strand, within Pf6N2E2_1961 at 2326.406 kb on - strand, within Pf6N2E2_1961 at 2326.581 kb on + strand, within Pf6N2E2_1961 at 2326.582 kb on - strand, within Pf6N2E2_1961 at 2326.608 kb on + strand, within Pf6N2E2_1961 at 2326.609 kb on - strand, within Pf6N2E2_1961 at 2326.622 kb on - strand, within Pf6N2E2_1961 at 2326.748 kb on + strand at 2326.774 kb on + strand at 2326.774 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3 remove 2,323,800 + +0.3 2,323,804 + -0.7 2,323,805 - -0.7 2,323,805 - -0.7 2,323,805 - +0.3 2,323,824 + +0.3 2,323,999 - +0.3 2,324,016 + -1.2 2,324,123 + Pf6N2E2_1959 0.20 +0.8 2,324,251 + Pf6N2E2_1959 0.37 +0.3 2,324,578 - Pf6N2E2_1959 0.79 +0.3 2,324,637 - Pf6N2E2_1959 0.87 +0.3 2,324,664 + +0.3 2,324,664 + +1.3 2,324,664 + +0.3 2,324,665 - -1.3 2,324,665 - +0.3 2,324,665 - +0.3 2,324,665 - -0.3 2,324,722 + -0.5 2,324,748 - -0.7 2,324,881 - +0.3 2,324,881 - +0.3 2,325,232 - Pf6N2E2_1960 0.40 -0.7 2,325,232 - Pf6N2E2_1960 0.40 +0.3 2,325,330 + Pf6N2E2_1960 0.48 +0.3 2,325,331 - Pf6N2E2_1960 0.49 -0.7 2,325,331 - Pf6N2E2_1960 0.49 -0.7 2,325,355 + Pf6N2E2_1960 0.51 -0.7 2,325,387 - Pf6N2E2_1960 0.54 +0.3 2,325,502 + Pf6N2E2_1960 0.64 +0.3 2,325,502 + Pf6N2E2_1960 0.64 +0.3 2,325,503 - Pf6N2E2_1960 0.64 +0.3 2,325,645 + Pf6N2E2_1960 0.77 +0.3 2,325,645 + Pf6N2E2_1960 0.77 -0.7 2,325,645 + Pf6N2E2_1960 0.77 -0.7 2,325,646 - Pf6N2E2_1960 0.77 -1.4 2,325,646 - Pf6N2E2_1960 0.77 -3.1 2,325,646 - Pf6N2E2_1960 0.77 -0.7 2,325,900 - +0.3 2,326,021 + +0.3 2,326,021 + -1.6 2,326,021 + +0.3 2,326,022 - +0.3 2,326,022 - +0.3 2,326,022 - -1.3 2,326,299 + Pf6N2E2_1961 0.41 +0.3 2,326,306 - Pf6N2E2_1961 0.42 -0.7 2,326,345 - Pf6N2E2_1961 0.47 +0.3 2,326,345 - Pf6N2E2_1961 0.47 -0.7 2,326,345 - Pf6N2E2_1961 0.47 +0.8 2,326,401 + Pf6N2E2_1961 0.54 +0.3 2,326,401 + Pf6N2E2_1961 0.54 +0.3 2,326,401 + Pf6N2E2_1961 0.54 -2.4 2,326,401 + Pf6N2E2_1961 0.54 +0.3 2,326,401 + Pf6N2E2_1961 0.54 -0.7 2,326,401 + Pf6N2E2_1961 0.54 +0.3 2,326,405 + Pf6N2E2_1961 0.54 -0.7 2,326,406 - Pf6N2E2_1961 0.54 +0.3 2,326,406 - Pf6N2E2_1961 0.54 +0.3 2,326,581 + Pf6N2E2_1961 0.75 +1.3 2,326,582 - Pf6N2E2_1961 0.75 +0.3 2,326,608 + Pf6N2E2_1961 0.79 +0.3 2,326,609 - Pf6N2E2_1961 0.79 -0.7 2,326,622 - Pf6N2E2_1961 0.80 -0.1 2,326,748 + -1.3 2,326,774 + -0.7 2,326,774 + -0.8
Or see this region's nucleotide sequence