Experiment: Ying_all64 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1668 and Pf6N2E2_1669 are separated by 134 nucleotides Pf6N2E2_1669 and Pf6N2E2_1670 are separated by 62 nucleotides Pf6N2E2_1670 and Pf6N2E2_1671 are separated by 24 nucleotides Pf6N2E2_1671 and Pf6N2E2_1672 are separated by 148 nucleotides
Pf6N2E2_1668 - Xylonate dehydratase (EC 4.2.1.82) (from data) , at 1,974,601 to 1,976,385
_1668
Pf6N2E2_1669 - Probable tautomerase, at 1,976,520 to 1,976,738
_1669
Pf6N2E2_1670 - conserved membrane protein, at 1,976,801 to 1,977,310
_1670
Pf6N2E2_1671 - Transcriptional regulator, TetR family, at 1,977,335 to 1,978,012
_1671
Pf6N2E2_1672 - hypothetical protein, at 1,978,161 to 1,979,042
_1672
Position (kb)
1976
1977
1978 Strain fitness (log2 ratio)
-2
-1
0
1 at 1975.825 kb on + strand, within Pf6N2E2_1668 at 1975.825 kb on + strand, within Pf6N2E2_1668 at 1975.825 kb on + strand, within Pf6N2E2_1668 at 1975.826 kb on - strand, within Pf6N2E2_1668 at 1975.826 kb on - strand, within Pf6N2E2_1668 at 1975.826 kb on - strand, within Pf6N2E2_1668 at 1975.851 kb on + strand, within Pf6N2E2_1668 at 1975.852 kb on - strand, within Pf6N2E2_1668 at 1975.852 kb on - strand, within Pf6N2E2_1668 at 1975.874 kb on + strand, within Pf6N2E2_1668 at 1975.874 kb on + strand, within Pf6N2E2_1668 at 1975.894 kb on + strand, within Pf6N2E2_1668 at 1975.895 kb on - strand, within Pf6N2E2_1668 at 1976.247 kb on + strand at 1976.282 kb on - strand at 1976.441 kb on + strand at 1976.441 kb on + strand at 1976.441 kb on + strand at 1976.442 kb on - strand at 1976.501 kb on + strand at 1976.535 kb on + strand at 1976.536 kb on - strand at 1976.536 kb on - strand at 1976.536 kb on - strand at 1976.613 kb on + strand, within Pf6N2E2_1669 at 1976.744 kb on - strand at 1976.750 kb on + strand at 1976.922 kb on - strand, within Pf6N2E2_1670 at 1976.922 kb on - strand, within Pf6N2E2_1670 at 1977.182 kb on + strand, within Pf6N2E2_1670 at 1977.183 kb on - strand, within Pf6N2E2_1670 at 1977.211 kb on - strand, within Pf6N2E2_1670 at 1977.258 kb on - strand, within Pf6N2E2_1670 at 1977.284 kb on + strand at 1977.284 kb on + strand at 1977.390 kb on + strand at 1977.390 kb on + strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.391 kb on - strand at 1977.471 kb on - strand at 1977.471 kb on - strand, within Pf6N2E2_1671 at 1977.471 kb on - strand, within Pf6N2E2_1671 at 1977.472 kb on + strand, within Pf6N2E2_1671 at 1977.605 kb on + strand, within Pf6N2E2_1671 at 1977.716 kb on + strand, within Pf6N2E2_1671 at 1977.716 kb on + strand, within Pf6N2E2_1671 at 1977.717 kb on - strand, within Pf6N2E2_1671 at 1977.828 kb on - strand, within Pf6N2E2_1671 at 1977.837 kb on + strand, within Pf6N2E2_1671 at 1978.027 kb on - strand at 1978.029 kb on - strand at 1978.029 kb on - strand at 1978.029 kb on - strand at 1978.035 kb on - strand at 1978.063 kb on + strand at 1978.063 kb on + strand at 1978.067 kb on + strand at 1978.067 kb on + strand at 1978.068 kb on - strand at 1978.068 kb on - strand at 1978.092 kb on + strand at 1978.093 kb on - strand at 1978.143 kb on + strand at 1978.143 kb on + strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.144 kb on - strand at 1978.154 kb on + strand at 1978.155 kb on - strand at 1978.155 kb on - strand at 1978.174 kb on + strand at 1978.191 kb on + strand at 1978.254 kb on + strand, within Pf6N2E2_1672
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3 remove 1,975,825 + Pf6N2E2_1668 0.69 +0.2 1,975,825 + Pf6N2E2_1668 0.69 -0.7 1,975,825 + Pf6N2E2_1668 0.69 +0.2 1,975,826 - Pf6N2E2_1668 0.69 +1.2 1,975,826 - Pf6N2E2_1668 0.69 +0.2 1,975,826 - Pf6N2E2_1668 0.69 -1.4 1,975,851 + Pf6N2E2_1668 0.70 +0.2 1,975,852 - Pf6N2E2_1668 0.70 -0.8 1,975,852 - Pf6N2E2_1668 0.70 +0.2 1,975,874 + Pf6N2E2_1668 0.71 -1.2 1,975,874 + Pf6N2E2_1668 0.71 +0.2 1,975,894 + Pf6N2E2_1668 0.72 +0.2 1,975,895 - Pf6N2E2_1668 0.72 -0.4 1,976,247 + +0.2 1,976,282 - -2.3 1,976,441 + -1.3 1,976,441 + +0.2 1,976,441 + +0.2 1,976,442 - +0.2 1,976,501 + -1.4 1,976,535 + -1.4 1,976,536 - -1.1 1,976,536 - +0.2 1,976,536 - +0.2 1,976,613 + Pf6N2E2_1669 0.42 -1.6 1,976,744 - +1.2 1,976,750 + -0.8 1,976,922 - Pf6N2E2_1670 0.24 -1.2 1,976,922 - Pf6N2E2_1670 0.24 -0.8 1,977,182 + Pf6N2E2_1670 0.75 +0.2 1,977,183 - Pf6N2E2_1670 0.75 -0.8 1,977,211 - Pf6N2E2_1670 0.80 +0.2 1,977,258 - Pf6N2E2_1670 0.90 -1.4 1,977,284 + +0.2 1,977,284 + -0.7 1,977,390 + -0.8 1,977,390 + +0.2 1,977,391 - +0.2 1,977,391 - +0.2 1,977,391 - -0.8 1,977,391 - +0.8 1,977,471 - +0.2 1,977,471 - Pf6N2E2_1671 0.20 +0.2 1,977,471 - Pf6N2E2_1671 0.20 -1.4 1,977,472 + Pf6N2E2_1671 0.20 +0.2 1,977,605 + Pf6N2E2_1671 0.40 +0.2 1,977,716 + Pf6N2E2_1671 0.56 +0.2 1,977,716 + Pf6N2E2_1671 0.56 +0.2 1,977,717 - Pf6N2E2_1671 0.56 -0.1 1,977,828 - Pf6N2E2_1671 0.73 +0.2 1,977,837 + Pf6N2E2_1671 0.74 +0.2 1,978,027 - +0.2 1,978,029 - +0.2 1,978,029 - +0.2 1,978,029 - -1.4 1,978,035 - -0.5 1,978,063 + +0.2 1,978,063 + +0.2 1,978,067 + +0.2 1,978,067 + -0.3 1,978,068 - +0.2 1,978,068 - +0.2 1,978,092 + +0.2 1,978,093 - -1.6 1,978,143 + +0.2 1,978,143 + +0.2 1,978,144 - +0.2 1,978,144 - +0.5 1,978,144 - -1.4 1,978,154 + +0.2 1,978,155 - +0.2 1,978,155 - +0.2 1,978,174 + -0.1 1,978,191 + -2.1 1,978,254 + Pf6N2E2_1672 0.11 +0.2
Or see this region's nucleotide sequence