Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1650

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1649 and Pf6N2E2_1650 overlap by 4 nucleotidesPf6N2E2_1650 and Pf6N2E2_1651 are separated by 84 nucleotides Pf6N2E2_1649 - Maltose/maltodextrin transport ATP-binding protein MalK (EC 3.6.3.19), at 1,954,691 to 1,955,845 _1649 Pf6N2E2_1650 - Sucrose alpha-glucosidase (EC 3.2.1.48) (from data), at 1,955,842 to 1,957,344 _1650 Pf6N2E2_1651 - Sucrose specific transcriptional regulator CscR, LacI family, at 1,957,429 to 1,958,412 _1651 Position (kb) 1955 1956 1957 1958Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1954.941 kb on - strand, within Pf6N2E2_1649at 1954.949 kb on + strand, within Pf6N2E2_1649at 1955.255 kb on + strand, within Pf6N2E2_1649at 1955.255 kb on + strand, within Pf6N2E2_1649at 1955.255 kb on + strand, within Pf6N2E2_1649at 1955.256 kb on - strand, within Pf6N2E2_1649at 1955.355 kb on - strand, within Pf6N2E2_1649at 1955.355 kb on - strand, within Pf6N2E2_1649at 1955.613 kb on - strand, within Pf6N2E2_1649at 1955.841 kb on + strandat 1955.841 kb on + strandat 1955.841 kb on + strandat 1955.842 kb on - strandat 1955.954 kb on - strandat 1956.031 kb on + strand, within Pf6N2E2_1650at 1956.032 kb on - strand, within Pf6N2E2_1650at 1956.032 kb on - strand, within Pf6N2E2_1650at 1956.032 kb on - strand, within Pf6N2E2_1650at 1956.070 kb on + strand, within Pf6N2E2_1650at 1956.070 kb on + strand, within Pf6N2E2_1650at 1956.071 kb on - strand, within Pf6N2E2_1650at 1956.209 kb on - strand, within Pf6N2E2_1650at 1956.508 kb on + strand, within Pf6N2E2_1650at 1956.508 kb on + strand, within Pf6N2E2_1650at 1956.509 kb on - strand, within Pf6N2E2_1650at 1956.563 kb on - strand, within Pf6N2E2_1650at 1956.563 kb on - strand, within Pf6N2E2_1650at 1956.592 kb on + strand, within Pf6N2E2_1650at 1956.610 kb on + strand, within Pf6N2E2_1650at 1956.611 kb on - strand, within Pf6N2E2_1650at 1957.060 kb on + strand, within Pf6N2E2_1650at 1957.061 kb on - strand, within Pf6N2E2_1650at 1957.063 kb on + strand, within Pf6N2E2_1650at 1957.176 kb on - strand, within Pf6N2E2_1650at 1957.243 kb on + strandat 1957.275 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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1,954,941 - Pf6N2E2_1649 0.22 +1.1
1,954,949 + Pf6N2E2_1649 0.22 +0.2
1,955,255 + Pf6N2E2_1649 0.49 -0.8
1,955,255 + Pf6N2E2_1649 0.49 +0.2
1,955,255 + Pf6N2E2_1649 0.49 +0.2
1,955,256 - Pf6N2E2_1649 0.49 +0.2
1,955,355 - Pf6N2E2_1649 0.57 +0.2
1,955,355 - Pf6N2E2_1649 0.57 -0.8
1,955,613 - Pf6N2E2_1649 0.80 +0.2
1,955,841 + +0.2
1,955,841 + +0.2
1,955,841 + +0.2
1,955,842 - -0.2
1,955,954 - -0.8
1,956,031 + Pf6N2E2_1650 0.13 +0.2
1,956,032 - Pf6N2E2_1650 0.13 -2.0
1,956,032 - Pf6N2E2_1650 0.13 +0.2
1,956,032 - Pf6N2E2_1650 0.13 +0.2
1,956,070 + Pf6N2E2_1650 0.15 -1.4
1,956,070 + Pf6N2E2_1650 0.15 -3.8
1,956,071 - Pf6N2E2_1650 0.15 -3.5
1,956,209 - Pf6N2E2_1650 0.24 -0.8
1,956,508 + Pf6N2E2_1650 0.44 +0.2
1,956,508 + Pf6N2E2_1650 0.44 +0.2
1,956,509 - Pf6N2E2_1650 0.44 +0.2
1,956,563 - Pf6N2E2_1650 0.48 +0.2
1,956,563 - Pf6N2E2_1650 0.48 +0.2
1,956,592 + Pf6N2E2_1650 0.50 +0.2
1,956,610 + Pf6N2E2_1650 0.51 +0.2
1,956,611 - Pf6N2E2_1650 0.51 +0.2
1,957,060 + Pf6N2E2_1650 0.81 -4.4
1,957,061 - Pf6N2E2_1650 0.81 +0.2
1,957,063 + Pf6N2E2_1650 0.81 -2.1
1,957,176 - Pf6N2E2_1650 0.89 -3.6
1,957,243 + -2.2
1,957,275 - +0.2

Or see this region's nucleotide sequence