Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_160

Experiment: Ying_all64 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_158 and Pf6N2E2_159 are separated by 5 nucleotidesPf6N2E2_159 and Pf6N2E2_160 are separated by 248 nucleotidesPf6N2E2_160 and Pf6N2E2_161 are separated by 133 nucleotides Pf6N2E2_158 - Endonuclease I precursor (EC 3.1.21.1), at 212,509 to 213,198 _158 Pf6N2E2_159 - DNA damage resistance protein (from data), at 213,204 to 213,452 _159 Pf6N2E2_160 - hypothetical protein, at 213,701 to 214,147 _160 Pf6N2E2_161 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 214,281 to 215,237 _161 Position (kb) 213 214 215Strain fitness (log2 ratio) -3 -2 -1 0 1at 212.882 kb on + strand, within Pf6N2E2_158at 212.882 kb on + strand, within Pf6N2E2_158at 212.883 kb on - strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.967 kb on + strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 212.968 kb on - strand, within Pf6N2E2_158at 213.013 kb on + strand, within Pf6N2E2_158at 213.014 kb on - strand, within Pf6N2E2_158at 213.014 kb on - strand, within Pf6N2E2_158at 213.017 kb on + strand, within Pf6N2E2_158at 213.018 kb on - strand, within Pf6N2E2_158at 213.018 kb on - strand, within Pf6N2E2_158at 213.053 kb on + strand, within Pf6N2E2_158at 213.053 kb on + strand, within Pf6N2E2_158at 213.053 kb on + strand, within Pf6N2E2_158at 213.058 kb on - strand, within Pf6N2E2_158at 213.058 kb on - strand, within Pf6N2E2_158at 213.068 kb on + strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.069 kb on - strand, within Pf6N2E2_158at 213.092 kb on + strand, within Pf6N2E2_158at 213.092 kb on + strand, within Pf6N2E2_158at 213.411 kb on - strand, within Pf6N2E2_159at 213.411 kb on - strand, within Pf6N2E2_159at 213.412 kb on + strand, within Pf6N2E2_159at 213.412 kb on + strand, within Pf6N2E2_159at 213.412 kb on + strand, within Pf6N2E2_159at 213.413 kb on - strand, within Pf6N2E2_159at 213.413 kb on - strand, within Pf6N2E2_159at 213.420 kb on + strand, within Pf6N2E2_159at 213.421 kb on - strand, within Pf6N2E2_159at 213.428 kb on + strandat 213.580 kb on + strandat 213.592 kb on + strandat 213.592 kb on + strandat 213.593 kb on - strandat 213.995 kb on + strand, within Pf6N2E2_160at 213.996 kb on - strand, within Pf6N2E2_160at 214.069 kb on - strand, within Pf6N2E2_160at 214.135 kb on + strandat 214.135 kb on + strandat 214.135 kb on + strandat 214.135 kb on + strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.136 kb on - strandat 214.197 kb on + strandat 214.198 kb on - strandat 214.202 kb on + strandat 214.202 kb on + strandat 214.202 kb on + strandat 214.203 kb on - strandat 214.203 kb on - strandat 214.257 kb on + strandat 214.257 kb on + strandat 214.257 kb on + strandat 214.258 kb on - strandat 214.258 kb on - strandat 214.264 kb on - strandat 214.264 kb on - strandat 214.455 kb on + strand, within Pf6N2E2_161at 214.455 kb on + strand, within Pf6N2E2_161at 214.455 kb on + strand, within Pf6N2E2_161at 214.456 kb on - strand, within Pf6N2E2_161at 214.753 kb on - strand, within Pf6N2E2_161at 214.753 kb on - strand, within Pf6N2E2_161

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 3
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212,882 + Pf6N2E2_158 0.54 -1.2
212,882 + Pf6N2E2_158 0.54 +0.3
212,883 - Pf6N2E2_158 0.54 -0.7
212,967 + Pf6N2E2_158 0.66 +0.3
212,967 + Pf6N2E2_158 0.66 +0.2
212,967 + Pf6N2E2_158 0.66 +0.3
212,967 + Pf6N2E2_158 0.66 -0.7
212,967 + Pf6N2E2_158 0.66 -0.7
212,967 + Pf6N2E2_158 0.66 +0.3
212,968 - Pf6N2E2_158 0.67 +0.3
212,968 - Pf6N2E2_158 0.67 +0.3
212,968 - Pf6N2E2_158 0.67 -0.4
212,968 - Pf6N2E2_158 0.67 +0.3
212,968 - Pf6N2E2_158 0.67 +0.3
213,013 + Pf6N2E2_158 0.73 +0.3
213,014 - Pf6N2E2_158 0.73 +0.1
213,014 - Pf6N2E2_158 0.73 +0.3
213,017 + Pf6N2E2_158 0.74 -0.6
213,018 - Pf6N2E2_158 0.74 +0.3
213,018 - Pf6N2E2_158 0.74 -1.2
213,053 + Pf6N2E2_158 0.79 -0.7
213,053 + Pf6N2E2_158 0.79 -0.4
213,053 + Pf6N2E2_158 0.79 -0.7
213,058 - Pf6N2E2_158 0.80 -0.5
213,058 - Pf6N2E2_158 0.80 +0.3
213,068 + Pf6N2E2_158 0.81 -0.7
213,069 - Pf6N2E2_158 0.81 +1.3
213,069 - Pf6N2E2_158 0.81 +0.3
213,069 - Pf6N2E2_158 0.81 -1.2
213,069 - Pf6N2E2_158 0.81 +0.3
213,092 + Pf6N2E2_158 0.84 -1.2
213,092 + Pf6N2E2_158 0.84 -0.7
213,411 - Pf6N2E2_159 0.83 -1.5
213,411 - Pf6N2E2_159 0.83 -1.8
213,412 + Pf6N2E2_159 0.84 +0.3
213,412 + Pf6N2E2_159 0.84 +0.3
213,412 + Pf6N2E2_159 0.84 +0.3
213,413 - Pf6N2E2_159 0.84 +0.3
213,413 - Pf6N2E2_159 0.84 -0.7
213,420 + Pf6N2E2_159 0.87 -0.7
213,421 - Pf6N2E2_159 0.87 +0.3
213,428 + +0.3
213,580 + -0.6
213,592 + +0.3
213,592 + +0.3
213,593 - +0.3
213,995 + Pf6N2E2_160 0.66 +0.3
213,996 - Pf6N2E2_160 0.66 -1.4
214,069 - Pf6N2E2_160 0.82 -2.0
214,135 + +0.3
214,135 + -1.2
214,135 + -1.7
214,135 + -0.7
214,136 - +0.3
214,136 - +0.3
214,136 - +0.3
214,136 - -0.7
214,136 - -3.1
214,136 - -0.7
214,136 - +0.3
214,197 + +0.3
214,198 - -1.2
214,202 + -1.8
214,202 + -0.7
214,202 + -0.7
214,203 - -0.7
214,203 - -1.2
214,257 + +0.3
214,257 + -0.1
214,257 + -0.7
214,258 - -2.0
214,258 - -1.5
214,264 - -2.7
214,264 - +0.3
214,455 + Pf6N2E2_161 0.18 +0.3
214,455 + Pf6N2E2_161 0.18 +0.3
214,455 + Pf6N2E2_161 0.18 +0.3
214,456 - Pf6N2E2_161 0.18 -0.7
214,753 - Pf6N2E2_161 0.49 +0.3
214,753 - Pf6N2E2_161 0.49 +0.3

Or see this region's nucleotide sequence