Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS15540

Experiment: L-Isoleucine (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	L-Isoleucine (C)
3,477,777	+		PFLU_RS15535	0.32	+0.3
3,477,777	+		PFLU_RS15535	0.32	+1.0
3,477,778	-		PFLU_RS15535	0.32	+1.1
3,477,861	+		PFLU_RS15535	0.39	+0.7
3,477,861	+		PFLU_RS15535	0.39	+0.1
3,477,861	+		PFLU_RS15535	0.39	-0.5
3,477,862	-		PFLU_RS15535	0.39	+2.0
3,477,862	-		PFLU_RS15535	0.39	+0.0
3,477,862	-		PFLU_RS15535	0.39	+0.5
3,477,862	-		PFLU_RS15535	0.39	+1.1
3,477,897	+		PFLU_RS15535	0.42	-0.9
3,477,897	+		PFLU_RS15535	0.42	+0.7
3,477,898	-		PFLU_RS15535	0.42	-1.3
3,477,905	-		PFLU_RS15535	0.42	-2.5
3,477,905	-		PFLU_RS15535	0.42	+0.3
3,477,905	-		PFLU_RS15535	0.42	-0.0
3,477,932	-		PFLU_RS15535	0.45	-3.4
3,477,945	+		PFLU_RS15535	0.46	+1.9
3,477,945	+		PFLU_RS15535	0.46	-0.9
3,477,945	+		PFLU_RS15535	0.46	-2.5
3,477,946	-		PFLU_RS15535	0.46	+2.5
3,477,946	-		PFLU_RS15535	0.46	-0.4
3,478,025	+		PFLU_RS15535	0.52	+1.1
3,478,025	+		PFLU_RS15535	0.52	+0.6
3,478,026	-		PFLU_RS15535	0.52	+0.8
3,478,041	+		PFLU_RS15535	0.53	+0.3
3,478,041	+		PFLU_RS15535	0.53	-0.8
3,478,041	+		PFLU_RS15535	0.53	-1.0
3,478,041	+		PFLU_RS15535	0.53	-0.4
3,478,041	+		PFLU_RS15535	0.53	+1.6
3,478,042	-		PFLU_RS15535	0.53	+2.6
3,478,042	-		PFLU_RS15535	0.53	-2.0
3,478,042	-		PFLU_RS15535	0.53	-0.3
3,478,042	-		PFLU_RS15535	0.53	-1.9
3,478,262	-		PFLU_RS15535	0.71	+0.9
3,478,377	+		PFLU_RS15535	0.80	+1.7
3,478,378	-		PFLU_RS15535	0.80	-0.7
3,478,378	-		PFLU_RS15535	0.80	-1.2
3,478,378	-		PFLU_RS15535	0.80	+0.4
3,478,378	-		PFLU_RS15535	0.80	-0.9
3,478,417	+		PFLU_RS15535	0.83	-0.0
3,478,498	+		PFLU_RS15535	0.89	+0.1
3,478,499	-		PFLU_RS15535	0.90	+2.6
3,478,499	-		PFLU_RS15535	0.90	-0.5
3,478,513	+				+1.1
3,478,563	+				-0.5
3,478,563	+				-0.7
3,478,564	-				-0.1
3,478,621	+				+0.0
3,478,622	-				-1.7
3,478,673	+				+2.1
3,478,674	-				-3.2
3,478,674	-				+0.4
3,478,674	-				+3.9
3,478,677	+				-0.3
3,478,677	+				+0.8
3,478,678	-				+0.1
3,478,678	-				-0.5
3,478,678	-				+1.1
3,478,678	-				+1.1
3,478,702	+				-1.9
3,478,702	+				-1.1
3,478,703	-				-0.3
3,478,703	-				-0.5
3,478,745	-				-3.0
3,478,745	-				+0.5
3,478,766	+				+1.5
3,478,767	-				-2.9
3,478,767	-				-1.7
3,478,858	+		PFLU_RS15540	0.27	+0.4
3,478,859	-		PFLU_RS15540	0.27	-0.4
3,478,859	-		PFLU_RS15540	0.27	-2.2
3,478,859	-		PFLU_RS15540	0.27	-2.5
3,478,859	-		PFLU_RS15540	0.27	-0.2
3,478,859	-		PFLU_RS15540	0.27	+1.5
3,479,041	+		PFLU_RS15540	0.70	+0.1
3,479,041	+		PFLU_RS15540	0.70	+0.5
3,479,042	-		PFLU_RS15540	0.70	+2.4
3,479,042	-		PFLU_RS15540	0.70	+0.2
3,479,056	-		PFLU_RS15540	0.73	-0.3
3,479,095	-		PFLU_RS15540	0.83	+0.1
3,479,095	-		PFLU_RS15540	0.83	-1.0
3,479,095	-		PFLU_RS15540	0.83	-0.5
3,479,104	+		PFLU_RS15540	0.85	+0.1
3,479,118	-		PFLU_RS15540	0.88	+1.7
3,479,118	-		PFLU_RS15540	0.88	+0.0
3,479,118	-		PFLU_RS15540	0.88	+0.4
3,479,118	-		PFLU_RS15540	0.88	-0.5
3,479,131	+				-1.5
3,479,131	+				-1.3
3,479,131	+				-0.9
3,479,132	-				-1.2
3,479,132	-				+1.8
3,479,132	-				-2.2
3,479,132	-				-1.7
3,479,132	-				+1.1
3,479,132	-				+1.1
3,479,132	-				-1.0
3,479,253	+				-0.5
3,479,253	+				-2.8
3,479,253	+				-0.2
3,479,254	-				-1.2
3,479,254	-				+2.5
3,479,254	-				-2.8
3,479,315	+				-0.7
3,479,316	-				-2.0
3,479,334	-				-0.1
3,479,365	-		PFLU_RS30840	0.16	+2.5
3,479,365	-		PFLU_RS30840	0.16	-1.9
3,479,426	+		PFLU_RS30840	0.45	+1.9
3,479,426	+		PFLU_RS30840	0.45	+1.1
3,479,426	+		PFLU_RS30840	0.45	-0.7
3,479,426	+		PFLU_RS30840	0.45	-2.3
3,479,427	-		PFLU_RS30840	0.45	-1.0
3,479,427	-		PFLU_RS30840	0.45	+0.5
3,479,464	+		PFLU_RS30840	0.63	-0.9
3,479,464	+		PFLU_RS30840	0.63	-1.0
3,479,464	+		PFLU_RS30840	0.63	-0.1
3,479,465	-		PFLU_RS30840	0.63	+0.5
3,479,609	+				-0.9
3,479,609	+				+0.5
3,479,610	-				+0.8
3,479,659	+				-0.2
3,479,708	+				+0.0
3,479,709	-				+1.1
3,479,709	-				-0.9
3,479,709	-				+0.3
3,479,748	-				+0.9
3,479,809	+				+0.2
3,479,809	+				-0.2
3,479,809	+				+0.9
3,479,809	+				+0.5
3,479,809	+				-1.5
3,479,809	+				-1.5
3,479,810	-				+0.1
3,479,810	-				+1.1
3,479,810	-				-1.2
3,479,810	-				-0.6
3,479,811	+				+1.1
3,479,811	+				+0.4
3,479,811	+				+1.0
3,479,811	+				-0.2
3,479,811	+				+0.5
3,479,811	+				+0.7
3,479,812	-				-2.0
3,479,812	-				-0.9
3,479,812	-				+2.4
3,479,812	-				+0.6
3,479,812	-				+1.7
3,479,882	+				+0.6
3,479,882	+				-0.7
3,479,883	-				-2.8
3,479,883	-				-0.5
3,479,905	+				-2.0
3,479,905	+				+1.1
3,479,906	-				+1.2
3,479,906	-				-0.4
3,479,906	-				-1.0
3,479,906	-				+0.1
3,479,917	+				-0.9
3,479,917	+				+1.1
3,479,917	+				+2.1
3,479,917	+				+1.1
3,479,917	+				+0.3
3,479,917	+				+0.7
3,479,918	-				+0.8
3,479,918	-				+1.6
3,479,918	-				-1.7
3,479,918	-				-0.7
3,479,918	-				-1.0
3,479,918	-				-0.7
3,479,938	+				-0.3
3,479,938	+				-1.2
3,479,938	+				-1.0
3,479,938	+				-1.5
3,479,938	+				+0.9
3,479,939	-				-1.0
3,479,939	-				-0.4
3,479,939	-				+0.5
3,479,939	-				+0.7
3,479,939	-				-0.6
3,479,950	+				-3.0
3,479,950	+				-0.5
3,479,960	+				-2.5
3,479,992	+				+0.8
3,479,993	-				-1.3
3,479,993	-				-0.5
3,480,033	-				-1.9
3,480,033	-				+0.0
3,480,033	-				-1.1