Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS17245

Experiment: L-Isoleucine (C)

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	L-Isoleucine (C)
3,921,523	+		PFLU_RS17240	0.29	+1.4
3,921,524	-		PFLU_RS17240	0.30	+1.0
3,921,542	+		PFLU_RS17240	0.31	-0.3
3,921,543	-		PFLU_RS17240	0.31	+0.5
3,921,543	-		PFLU_RS17240	0.31	+0.0
3,921,543	-		PFLU_RS17240	0.31	-1.2
3,921,543	-		PFLU_RS17240	0.31	+0.6
3,921,543	-		PFLU_RS17240	0.31	-2.0
3,921,543	-		PFLU_RS17240	0.31	+0.1
3,921,674	+		PFLU_RS17240	0.45	-2.4
3,921,674	+		PFLU_RS17240	0.45	-0.5
3,921,675	-		PFLU_RS17240	0.45	-2.2
3,921,733	+		PFLU_RS17240	0.50	-2.1
3,921,734	-		PFLU_RS17240	0.51	-2.9
3,921,855	+		PFLU_RS17240	0.63	-0.9
3,921,855	+		PFLU_RS17240	0.63	-1.9
3,921,927	+		PFLU_RS17240	0.70	-0.9
3,921,927	+		PFLU_RS17240	0.70	-0.5
3,921,941	+		PFLU_RS17240	0.71	+0.7
3,921,941	+		PFLU_RS17240	0.71	-1.3
3,921,942	-		PFLU_RS17240	0.71	-1.7
3,922,007	+		PFLU_RS17240	0.78	-3.0
3,922,007	+		PFLU_RS17240	0.78	-1.7
3,922,007	+		PFLU_RS17240	0.78	-0.1
3,922,007	+		PFLU_RS17240	0.78	-2.3
3,922,007	+		PFLU_RS17240	0.78	-0.1
3,922,007	+		PFLU_RS17240	0.78	+1.7
3,922,007	+		PFLU_RS17240	0.78	+0.8
3,922,007	+		PFLU_RS17240	0.78	-1.3
3,922,007	+		PFLU_RS17240	0.78	+1.3
3,922,007	+		PFLU_RS17240	0.78	-1.5
3,922,007	+		PFLU_RS17240	0.78	+3.2
3,922,007	+		PFLU_RS17240	0.78	+1.3
3,922,007	+		PFLU_RS17240	0.78	+1.2
3,922,008	-		PFLU_RS17240	0.78	-0.4
3,922,008	-		PFLU_RS17240	0.78	-0.4
3,922,008	-		PFLU_RS17240	0.78	+0.1
3,922,008	-		PFLU_RS17240	0.78	+1.0
3,922,008	-		PFLU_RS17240	0.78	-1.3
3,922,008	-		PFLU_RS17240	0.78	-2.3
3,922,008	-		PFLU_RS17240	0.78	+0.5
3,922,008	-		PFLU_RS17240	0.78	-0.5
3,922,076	+		PFLU_RS17240	0.85	-2.0
3,922,076	+		PFLU_RS17240	0.85	-1.9
3,922,079	+		PFLU_RS17240	0.85	-2.5
3,922,171	+				-0.1
3,922,227	+				-1.7
3,922,227	+				+0.9
3,922,227	+				+1.0
3,922,227	+				-0.3
3,922,227	+				-1.7
3,922,227	+				+1.6
3,922,228	-				-1.0
3,922,228	-				-0.7
3,922,228	-				+0.7
3,922,228	-				-1.4
3,922,228	-				-2.0
3,922,228	-				-1.1
3,922,228	-				+1.5
3,922,359	-				-0.1
3,922,359	-				-1.9
3,922,363	+				+0.5
3,922,363	+				+1.5
3,922,363	+				-1.2
3,922,364	-				-2.4
3,922,364	-				+0.5
3,922,364	-				+1.1
3,922,439	+				-0.1
3,922,439	+				-1.7
3,922,439	+				-0.2
3,922,439	+				-0.5
3,922,439	+				+1.7
3,922,439	+				+0.5
3,922,440	-				+1.3
3,922,440	-				+2.3
3,922,440	-				-0.5
3,922,443	+				+0.7
3,922,444	-				-1.7
3,922,444	-				-0.9
3,922,493	+				+1.2
3,922,493	+				-1.5
3,922,494	-				-2.4
3,922,494	-				-1.3
3,922,494	-				-1.4
3,922,494	-				+0.1
3,922,494	-				+1.1
3,922,683	+		PFLU_RS17245	0.12	-0.3
3,922,683	+		PFLU_RS17245	0.12	+0.5
3,922,683	+		PFLU_RS17245	0.12	-0.5
3,922,683	+		PFLU_RS17245	0.12	+1.5
3,922,684	-		PFLU_RS17245	0.12	+1.5
3,922,752	+		PFLU_RS17245	0.17	+0.1
3,922,752	+		PFLU_RS17245	0.17	+1.1
3,922,752	+		PFLU_RS17245	0.17	-1.9
3,922,752	+		PFLU_RS17245	0.17	+3.5
3,922,791	+		PFLU_RS17245	0.19	-0.9
3,922,791	+		PFLU_RS17245	0.19	-0.3
3,922,792	-		PFLU_RS17245	0.19	+3.7
3,922,998	+		PFLU_RS17245	0.33	+0.6
3,922,998	+		PFLU_RS17245	0.33	-1.7
3,922,998	+		PFLU_RS17245	0.33	-0.2
3,922,998	+		PFLU_RS17245	0.33	+0.7
3,922,999	-		PFLU_RS17245	0.33	+1.2
3,922,999	-		PFLU_RS17245	0.33	+1.8
3,922,999	-		PFLU_RS17245	0.33	+1.3
3,922,999	-		PFLU_RS17245	0.33	+0.2
3,923,212	-		PFLU_RS17245	0.47	-0.7
3,923,346	+		PFLU_RS17245	0.55	-1.7
3,923,346	+		PFLU_RS17245	0.55	+2.0
3,923,385	+		PFLU_RS17245	0.58	+1.6
3,923,385	+		PFLU_RS17245	0.58	-0.3
3,923,386	-		PFLU_RS17245	0.58	+0.3
3,923,386	-		PFLU_RS17245	0.58	+0.5
3,923,458	+		PFLU_RS17245	0.63	+1.5
3,923,604	+		PFLU_RS17245	0.72	-1.3
3,923,604	+		PFLU_RS17245	0.72	-0.0
3,923,675	+		PFLU_RS17245	0.77	+0.9
3,923,676	-		PFLU_RS17245	0.77	-1.7
3,923,676	-		PFLU_RS17245	0.77	+2.1
3,923,712	+		PFLU_RS17245	0.79	-0.5
3,923,712	+		PFLU_RS17245	0.79	-1.7
3,923,712	+		PFLU_RS17245	0.79	+0.5
3,923,712	+		PFLU_RS17245	0.79	+0.0
3,923,712	+		PFLU_RS17245	0.79	+0.5
3,923,713	-		PFLU_RS17245	0.79	+1.7
3,923,713	-		PFLU_RS17245	0.79	-0.9
3,923,713	-		PFLU_RS17245	0.79	-1.5
3,923,713	-		PFLU_RS17245	0.79	+0.0
3,923,713	-		PFLU_RS17245	0.79	-0.9
3,923,713	-		PFLU_RS17245	0.79	+1.5
3,923,790	+		PFLU_RS17245	0.84	-1.1
3,923,791	-		PFLU_RS17245	0.84	+0.7
3,923,791	-		PFLU_RS17245	0.84	+1.0
3,923,791	-		PFLU_RS17245	0.84	+0.7
3,923,791	-		PFLU_RS17245	0.84	+0.5
3,923,856	+		PFLU_RS17245	0.88	+2.2
3,923,949	+				-0.5
3,923,949	+				+0.5
3,923,949	+				-0.3
3,923,949	+				-1.9
3,923,949	+				+0.4
3,923,949	+				-0.4
3,923,949	+				-2.0
3,923,949	+				-0.9
3,923,950	-				-0.1
3,923,950	-				-0.4
3,923,950	-				-0.5
3,924,211	+		PFLU_RS17250	0.20	-0.3
3,924,211	+		PFLU_RS17250	0.20	-2.3
3,924,348	-		PFLU_RS17250	0.36	+2.9
3,924,419	+		PFLU_RS17250	0.44	+0.5
3,924,500	+		PFLU_RS17250	0.53	-0.7
3,924,501	-		PFLU_RS17250	0.53	-0.9
3,924,617	-		PFLU_RS17250	0.66	+1.3
3,924,750	+		PFLU_RS17250	0.81	-0.3
3,924,750	+		PFLU_RS17250	0.81	-2.0
3,924,750	+		PFLU_RS17250	0.81	-1.1
3,924,750	+		PFLU_RS17250	0.81	+0.3
3,924,751	-		PFLU_RS17250	0.81	-2.4
3,924,751	-		PFLU_RS17250	0.81	+1.9
3,924,754	+		PFLU_RS17250	0.81	-1.3
3,924,754	+		PFLU_RS17250	0.81	-0.5
3,924,754	+		PFLU_RS17250	0.81	-1.5
3,924,754	+		PFLU_RS17250	0.81	-1.3
3,924,755	-		PFLU_RS17250	0.81	-1.1
3,924,791	+		PFLU_RS17250	0.86	+1.2
3,924,792	-		PFLU_RS17250	0.86	-2.0
3,924,831	-				+0.2