Experiment: D-Mannitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01415 and PFLU_RS01420 overlap by 4 nucleotides PFLU_RS01420 and PFLU_RS01425 overlap by 4 nucleotides PFLU_RS01425 and PFLU_RS01430 are separated by 151 nucleotides
PFLU_RS01415: PFLU_RS01415 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 313,923 to 314,468
_RS01415
PFLU_RS01420: PFLU_RS01420 - glucose-1-phosphate thymidylyltransferase RfbA, at 314,465 to 315,340
_RS01420
PFLU_RS01425: PFLU_RS01425 - dTDP-glucose 4,6-dehydratase, at 315,337 to 316,419
_RS01425
PFLU_RS01430: PFLU_RS01430 - aminotransferase, at 316,571 to 319,480
_RS01430
Position (kb) 315
316
317 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 315.355 kb on - strand at 315.355 kb on - strand at 315.355 kb on - strand at 315.355 kb on - strand at 315.355 kb on - strand at 315.386 kb on + strand at 315.387 kb on - strand at 315.387 kb on - strand at 316.448 kb on + strand at 316.449 kb on - strand at 316.449 kb on - strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.463 kb on + strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.464 kb on - strand at 316.528 kb on + strand at 316.528 kb on + strand at 316.529 kb on - strand at 316.529 kb on - strand at 316.529 kb on - strand at 316.529 kb on - strand at 316.529 kb on - strand at 316.529 kb on - strand at 316.539 kb on + strand at 316.540 kb on - strand at 316.540 kb on - strand at 316.541 kb on + strand at 316.541 kb on + strand at 316.541 kb on + strand at 316.541 kb on + strand at 316.541 kb on + strand at 316.541 kb on + strand at 316.542 kb on - strand at 316.542 kb on - strand at 316.542 kb on - strand at 316.542 kb on - strand at 316.542 kb on - strand at 316.542 kb on - strand at 316.658 kb on + strand at 316.658 kb on + strand at 316.709 kb on + strand at 316.709 kb on + strand at 316.709 kb on + strand at 316.709 kb on + strand at 316.709 kb on + strand at 316.709 kb on + strand at 316.709 kb on + strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.710 kb on - strand at 316.711 kb on + strand at 316.711 kb on + strand at 316.711 kb on + strand at 316.711 kb on + strand at 316.712 kb on - strand at 316.712 kb on - strand at 316.712 kb on - strand at 316.764 kb on - strand at 316.920 kb on - strand, within PFLU_RS01430 at 317.151 kb on + strand, within PFLU_RS01430 at 317.151 kb on + strand, within PFLU_RS01430 at 317.316 kb on + strand, within PFLU_RS01430 at 317.316 kb on + strand, within PFLU_RS01430 at 317.316 kb on + strand, within PFLU_RS01430 at 317.316 kb on + strand, within PFLU_RS01430 at 317.316 kb on + strand, within PFLU_RS01430 at 317.409 kb on + strand, within PFLU_RS01430
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannitol (C) remove 315,355 - +0.1 315,355 - +0.5 315,355 - -2.7 315,355 - -2.8 315,355 - -2.0 315,386 + +1.4 315,387 - +0.2 315,387 - -1.0 316,448 + -1.7 316,449 - +1.8 316,449 - +0.5 316,463 + +0.2 316,463 + +0.3 316,463 + -0.7 316,463 + -1.7 316,463 + +0.6 316,463 + +1.0 316,463 + -0.0 316,463 + +1.1 316,463 + -0.4 316,463 + +1.2 316,464 - -0.5 316,464 - -1.6 316,464 - +2.3 316,464 - +0.3 316,464 - -1.1 316,464 - -1.9 316,464 - -1.4 316,464 - +0.1 316,464 - +2.5 316,464 - +1.8 316,464 - +2.2 316,464 - +1.6 316,528 + -1.8 316,528 + -1.7 316,529 - -0.9 316,529 - -1.2 316,529 - +0.6 316,529 - -1.4 316,529 - -1.6 316,529 - -0.0 316,539 + +3.8 316,540 - +1.0 316,540 - +0.7 316,541 + -2.7 316,541 + +2.0 316,541 + -0.7 316,541 + -0.7 316,541 + -1.6 316,541 + +0.7 316,542 - +0.1 316,542 - +0.3 316,542 - -0.5 316,542 - +0.7 316,542 - -2.4 316,542 - -0.4 316,658 + +1.5 316,658 + -0.8 316,709 + +1.1 316,709 + +2.5 316,709 + -1.4 316,709 + -0.4 316,709 + +1.0 316,709 + -1.2 316,709 + -0.2 316,710 - +0.7 316,710 - -0.6 316,710 - +0.5 316,710 - +0.6 316,710 - -1.8 316,710 - -2.0 316,710 - +0.7 316,710 - -0.6 316,710 - -1.0 316,710 - +1.1 316,710 - -0.3 316,711 + -0.8 316,711 + -0.4 316,711 + -1.0 316,711 + +1.5 316,712 - -1.3 316,712 - -0.4 316,712 - +0.3 316,764 - -1.6 316,920 - PFLU_RS01430 0.12 +0.0 317,151 + PFLU_RS01430 0.20 -0.7 317,151 + PFLU_RS01430 0.20 -1.5 317,316 + PFLU_RS01430 0.26 +0.1 317,316 + PFLU_RS01430 0.26 +0.2 317,316 + PFLU_RS01430 0.26 +0.1 317,316 + PFLU_RS01430 0.26 -1.1 317,316 + PFLU_RS01430 0.26 -0.2 317,409 + PFLU_RS01430 0.29 +0.4
Or see this region's nucleotide sequence