Experiment: Outgrowth after Tn7 transformation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36015 and BPHYT_RS36020 are separated by 181 nucleotides BPHYT_RS36020 and BPHYT_RS36025 are separated by 150 nucleotides BPHYT_RS36025 and BPHYT_RS36030 are separated by 50 nucleotides BPHYT_RS36030 and BPHYT_RS36035 are separated by 44 nucleotides BPHYT_RS36035 and BPHYT_RS36040 are separated by 24 nucleotides
BPHYT_RS36015: BPHYT_RS36015 - hypothetical protein, at 33,974 to 34,153
_RS36015
BPHYT_RS36020: BPHYT_RS36020 - hypothetical protein, at 34,335 to 34,571
_RS36020
BPHYT_RS36025: BPHYT_RS36025 - hypothetical protein, at 34,722 to 35,090
_RS36025
BPHYT_RS36030: BPHYT_RS36030 - hypothetical protein, at 35,141 to 35,560
_RS36030
BPHYT_RS36035: BPHYT_RS36035 - hypothetical protein, at 35,605 to 36,003
_RS36035
BPHYT_RS36040: BPHYT_RS36040 - hypothetical protein, at 36,028 to 36,657
_RS36040
Position (kb) 35
36 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 34.153 kb on + strand at 34.154 kb on - strand at 34.273 kb on - strand at 34.731 kb on - strand at 34.880 kb on + strand, within BPHYT_RS36025 at 34.881 kb on - strand, within BPHYT_RS36025 at 34.881 kb on - strand, within BPHYT_RS36025 at 34.924 kb on - strand, within BPHYT_RS36025 at 35.013 kb on - strand, within BPHYT_RS36025 at 35.013 kb on - strand, within BPHYT_RS36025 at 35.362 kb on - strand, within BPHYT_RS36030 at 35.418 kb on - strand, within BPHYT_RS36030 at 35.692 kb on - strand, within BPHYT_RS36035 at 35.789 kb on + strand, within BPHYT_RS36035 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040 at 36.250 kb on + strand, within BPHYT_RS36040
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth after Tn7 transformation remove 34,153 + +0.1 34,154 - -1.2 34,273 - -0.4 34,731 - +1.4 34,880 + BPHYT_RS36025 0.43 +0.5 34,881 - BPHYT_RS36025 0.43 -0.4 34,881 - BPHYT_RS36025 0.43 -0.2 34,924 - BPHYT_RS36025 0.55 +0.7 35,013 - BPHYT_RS36025 0.79 +0.2 35,013 - BPHYT_RS36025 0.79 -1.9 35,362 - BPHYT_RS36030 0.53 -0.7 35,418 - BPHYT_RS36030 0.66 -0.2 35,692 - BPHYT_RS36035 0.22 +0.1 35,789 + BPHYT_RS36035 0.46 -1.9 36,250 + BPHYT_RS36040 0.35 +3.2 36,250 + BPHYT_RS36040 0.35 -0.1 36,250 + BPHYT_RS36040 0.35 -0.4 36,250 + BPHYT_RS36040 0.35 +0.3
Or see this region's nucleotide sequence