Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11555

Experiment: Outgrowth after Tn7 transformation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11545 and BPHYT_RS11550 are separated by 76 nucleotidesBPHYT_RS11550 and BPHYT_RS11555 overlap by 4 nucleotidesBPHYT_RS11555 and BPHYT_RS11560 are separated by 239 nucleotides BPHYT_RS11545: BPHYT_RS11545 - allantoicase, at 2,624,807 to 2,625,820 _RS11545 BPHYT_RS11550: BPHYT_RS11550 - OHCU decarboxylase, at 2,625,897 to 2,626,418 _RS11550 BPHYT_RS11555: BPHYT_RS11555 - allantoinase, at 2,626,415 to 2,627,365 _RS11555 BPHYT_RS11560: BPHYT_RS11560 - Asp/Glu racemase, at 2,627,605 to 2,628,471 _RS11560 Position (kb) 2626 2627 2628Strain fitness (log2 ratio) -2 -1 0 1at 2625.655 kb on - strand, within BPHYT_RS11545at 2625.898 kb on + strandat 2626.623 kb on + strand, within BPHYT_RS11555at 2626.872 kb on + strand, within BPHYT_RS11555at 2627.134 kb on - strand, within BPHYT_RS11555at 2627.140 kb on - strand, within BPHYT_RS11555at 2627.346 kb on + strandat 2627.445 kb on + strandat 2627.583 kb on - strandat 2627.597 kb on - strandat 2627.995 kb on - strand, within BPHYT_RS11560

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Per-strain Table

Position Strand Gene LocusTag Fraction Outgrowth after Tn7 transformation
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2,625,655 - BPHYT_RS11545 0.84 -0.4
2,625,898 + +0.2
2,626,623 + BPHYT_RS11555 0.22 -0.9
2,626,872 + BPHYT_RS11555 0.48 -1.3
2,627,134 - BPHYT_RS11555 0.76 -0.2
2,627,140 - BPHYT_RS11555 0.76 -2.1
2,627,346 + -2.0
2,627,445 + -0.1
2,627,583 - -1.1
2,627,597 - -0.3
2,627,995 - BPHYT_RS11560 0.45 -0.9

Or see this region's nucleotide sequence