Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20285

Experiment: Outgrowth after Tn7 transformation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20280 and BPHYT_RS20285 are separated by 163 nucleotidesBPHYT_RS20285 and BPHYT_RS20290 are separated by 289 nucleotides BPHYT_RS20280: BPHYT_RS20280 - hypothetical protein, at 108,869 to 109,711 _RS20280 BPHYT_RS20285: BPHYT_RS20285 - MFS transporter, at 109,875 to 111,341 _RS20285 BPHYT_RS20290: BPHYT_RS20290 - LysR family transcriptional regulator, at 111,631 to 112,569 _RS20290 Position (kb) 109 110 111 112Strain fitness (log2 ratio) -1 0 1at 108.891 kb on - strandat 108.944 kb on + strandat 108.944 kb on + strandat 108.962 kb on + strand, within BPHYT_RS20280at 109.407 kb on - strand, within BPHYT_RS20280at 109.512 kb on - strand, within BPHYT_RS20280at 109.781 kb on - strandat 109.784 kb on + strandat 109.919 kb on - strandat 109.919 kb on - strandat 110.299 kb on - strand, within BPHYT_RS20285at 110.357 kb on + strand, within BPHYT_RS20285at 110.503 kb on + strand, within BPHYT_RS20285at 110.504 kb on - strand, within BPHYT_RS20285at 110.784 kb on - strand, within BPHYT_RS20285at 110.942 kb on + strand, within BPHYT_RS20285at 111.826 kb on + strand, within BPHYT_RS20290at 111.829 kb on + strand, within BPHYT_RS20290

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Per-strain Table

Position Strand Gene LocusTag Fraction Outgrowth after Tn7 transformation
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108,891 - -0.4
108,944 + -0.3
108,944 + +0.4
108,962 + BPHYT_RS20280 0.11 +1.0
109,407 - BPHYT_RS20280 0.64 -0.5
109,512 - BPHYT_RS20280 0.76 +0.2
109,781 - -1.1
109,784 + -0.2
109,919 - +0.1
109,919 - -0.1
110,299 - BPHYT_RS20285 0.29 -0.2
110,357 + BPHYT_RS20285 0.33 -0.1
110,503 + BPHYT_RS20285 0.43 -0.8
110,504 - BPHYT_RS20285 0.43 -1.2
110,784 - BPHYT_RS20285 0.62 +0.2
110,942 + BPHYT_RS20285 0.73 -0.1
111,826 + BPHYT_RS20290 0.21 -0.4
111,829 + BPHYT_RS20290 0.21 +0.3

Or see this region's nucleotide sequence