Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14705 and BWI76_RS14710 are separated by 919 nucleotides BWI76_RS14710 and BWI76_RS14715 are separated by 49 nucleotides BWI76_RS14715 and BWI76_RS14720 overlap by 20 nucleotides
BWI76_RS14705: BWI76_RS14705 - SefA, at 3,087,495 to 3,088,040
_RS14705
BWI76_RS14710: BWI76_RS14710 - FAD-dependent oxidoreductase, at 3,088,960 to 3,090,282
_RS14710
BWI76_RS14715: BWI76_RS14715 - type I methionyl aminopeptidase, at 3,090,332 to 3,091,111
_RS14715
BWI76_RS14720: BWI76_RS14720 - hypothetical protein, at 3,091,092 to 3,091,310
_RS14720
Position (kb)
3088
3089
3090
3091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3087.971 kb on - strand, within BWI76_RS14705 at 3087.972 kb on + strand, within BWI76_RS14705 at 3087.990 kb on + strand at 3087.997 kb on + strand at 3087.997 kb on + strand at 3087.997 kb on + strand at 3087.997 kb on - strand at 3087.997 kb on - strand at 3087.997 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.005 kb on - strand at 3088.007 kb on - strand at 3088.016 kb on - strand at 3088.099 kb on - strand at 3088.110 kb on - strand at 3088.271 kb on - strand at 3088.284 kb on + strand at 3088.521 kb on + strand at 3088.529 kb on - strand at 3088.539 kb on - strand at 3088.574 kb on + strand at 3088.585 kb on + strand at 3088.590 kb on + strand at 3088.591 kb on + strand at 3088.623 kb on - strand at 3088.666 kb on + strand at 3088.666 kb on + strand at 3088.703 kb on - strand at 3088.773 kb on - strand at 3088.800 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.825 kb on + strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.833 kb on - strand at 3088.847 kb on + strand at 3088.855 kb on - strand at 3088.855 kb on - strand at 3088.855 kb on - strand at 3088.859 kb on + strand at 3088.859 kb on + strand at 3088.864 kb on + strand at 3088.864 kb on + strand at 3088.867 kb on - strand at 3088.867 kb on - strand at 3088.872 kb on - strand at 3088.914 kb on + strand at 3088.919 kb on + strand at 3089.023 kb on - strand at 3089.052 kb on + strand at 3089.060 kb on - strand at 3089.120 kb on + strand, within BWI76_RS14710 at 3089.141 kb on - strand, within BWI76_RS14710 at 3089.227 kb on - strand, within BWI76_RS14710 at 3089.405 kb on + strand, within BWI76_RS14710 at 3089.424 kb on + strand, within BWI76_RS14710 at 3089.836 kb on - strand, within BWI76_RS14710 at 3090.044 kb on - strand, within BWI76_RS14710 at 3090.516 kb on - strand, within BWI76_RS14715 at 3090.652 kb on - strand, within BWI76_RS14715 at 3090.693 kb on + strand, within BWI76_RS14715 at 3091.003 kb on - strand, within BWI76_RS14715 at 3091.020 kb on - strand, within BWI76_RS14715
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,087,971 - BWI76_RS14705 0.87 +0.7 3,087,972 + BWI76_RS14705 0.87 -0.5 3,087,990 + -1.8 3,087,997 + -1.9 3,087,997 + +0.3 3,087,997 + +0.0 3,087,997 - +1.2 3,087,997 - -1.1 3,087,997 - -0.4 3,088,005 - +1.1 3,088,005 - +0.4 3,088,005 - -1.8 3,088,005 - +0.2 3,088,005 - -2.8 3,088,005 - -0.3 3,088,007 - -2.5 3,088,016 - +0.7 3,088,099 - -0.6 3,088,110 - +1.1 3,088,271 - +3.2 3,088,284 + +0.2 3,088,521 + +0.6 3,088,529 - -0.7 3,088,539 - +0.3 3,088,574 + -1.6 3,088,585 + +0.9 3,088,590 + -0.9 3,088,591 + +1.1 3,088,623 - -0.1 3,088,666 + -0.7 3,088,666 + -0.0 3,088,703 - +0.0 3,088,773 - +0.3 3,088,800 + -1.4 3,088,825 + +0.3 3,088,825 + -0.0 3,088,825 + -2.4 3,088,825 + +0.1 3,088,825 + -0.1 3,088,825 + -0.0 3,088,825 + +0.9 3,088,825 + -0.7 3,088,825 + +1.4 3,088,825 + +0.3 3,088,825 + -0.8 3,088,833 - +0.2 3,088,833 - -0.5 3,088,833 - -0.3 3,088,833 - +1.7 3,088,833 - +0.9 3,088,833 - -1.2 3,088,833 - -0.1 3,088,833 - +0.1 3,088,833 - -1.3 3,088,833 - +0.7 3,088,847 + +0.2 3,088,855 - -0.9 3,088,855 - -1.5 3,088,855 - +0.4 3,088,859 + +3.8 3,088,859 + +0.2 3,088,864 + +0.2 3,088,864 + +0.2 3,088,867 - +0.3 3,088,867 - +0.7 3,088,872 - -0.6 3,088,914 + -0.7 3,088,919 + +0.9 3,089,023 - +0.4 3,089,052 + +0.2 3,089,060 - -1.7 3,089,120 + BWI76_RS14710 0.12 -0.7 3,089,141 - BWI76_RS14710 0.14 +0.6 3,089,227 - BWI76_RS14710 0.20 +0.4 3,089,405 + BWI76_RS14710 0.34 +0.2 3,089,424 + BWI76_RS14710 0.35 +0.2 3,089,836 - BWI76_RS14710 0.66 +2.8 3,090,044 - BWI76_RS14710 0.82 +0.2 3,090,516 - BWI76_RS14715 0.24 -1.4 3,090,652 - BWI76_RS14715 0.41 +0.1 3,090,693 + BWI76_RS14715 0.46 -0.9 3,091,003 - BWI76_RS14715 0.86 +1.3 3,091,020 - BWI76_RS14715 0.88 +0.2
Or see this region's nucleotide sequence