Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15475 and BWI76_RS15480 are separated by 90 nucleotides BWI76_RS15480 and BWI76_RS15485 are separated by 51 nucleotides BWI76_RS15485 and BWI76_RS15490 are separated by 278 nucleotides
BWI76_RS15475: BWI76_RS15475 - NAD(P)-dependent oxidoreductase, at 3,242,553 to 3,243,302
_RS15475
BWI76_RS15480: BWI76_RS15480 - GntR family transcriptional regulator, at 3,243,393 to 3,244,079
_RS15480
BWI76_RS15485: BWI76_RS15485 - putative universal stress protein G, at 3,244,131 to 3,244,562
_RS15485
BWI76_RS15490: BWI76_RS15490 - mannitol dehydrogenase family protein, at 3,244,841 to 3,246,304
_RS15490
Position (kb)
3244
3245 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3243.241 kb on - strand at 3243.323 kb on + strand at 3243.475 kb on + strand, within BWI76_RS15480 at 3243.631 kb on - strand, within BWI76_RS15480 at 3243.646 kb on - strand, within BWI76_RS15480 at 3243.752 kb on + strand, within BWI76_RS15480 at 3243.776 kb on - strand, within BWI76_RS15480 at 3243.846 kb on + strand, within BWI76_RS15480 at 3243.846 kb on + strand, within BWI76_RS15480 at 3243.895 kb on + strand, within BWI76_RS15480 at 3243.895 kb on + strand, within BWI76_RS15480 at 3243.919 kb on - strand, within BWI76_RS15480 at 3243.926 kb on - strand, within BWI76_RS15480 at 3243.976 kb on + strand, within BWI76_RS15480 at 3243.978 kb on - strand, within BWI76_RS15480 at 3243.985 kb on - strand, within BWI76_RS15480 at 3243.985 kb on - strand, within BWI76_RS15480 at 3244.003 kb on + strand, within BWI76_RS15480 at 3244.011 kb on - strand at 3244.011 kb on - strand at 3244.011 kb on - strand at 3244.011 kb on - strand at 3244.094 kb on + strand at 3244.124 kb on - strand at 3244.212 kb on - strand, within BWI76_RS15485 at 3244.276 kb on - strand, within BWI76_RS15485 at 3244.320 kb on - strand, within BWI76_RS15485 at 3244.332 kb on - strand, within BWI76_RS15485 at 3244.338 kb on + strand, within BWI76_RS15485 at 3244.346 kb on - strand, within BWI76_RS15485 at 3244.346 kb on - strand, within BWI76_RS15485 at 3244.357 kb on + strand, within BWI76_RS15485 at 3244.382 kb on - strand, within BWI76_RS15485 at 3244.382 kb on - strand, within BWI76_RS15485 at 3244.439 kb on + strand, within BWI76_RS15485 at 3244.452 kb on - strand, within BWI76_RS15485 at 3244.452 kb on - strand, within BWI76_RS15485 at 3244.452 kb on - strand, within BWI76_RS15485 at 3244.452 kb on - strand, within BWI76_RS15485 at 3244.494 kb on + strand, within BWI76_RS15485 at 3244.520 kb on - strand at 3244.540 kb on - strand at 3244.540 kb on - strand at 3244.853 kb on + strand at 3244.853 kb on + strand at 3244.854 kb on + strand at 3244.861 kb on - strand at 3244.862 kb on - strand at 3244.873 kb on + strand at 3244.873 kb on + strand at 3244.873 kb on + strand at 3244.873 kb on + strand at 3244.881 kb on - strand at 3244.881 kb on - strand at 3244.881 kb on - strand at 3244.913 kb on - strand at 3244.935 kb on + strand at 3244.940 kb on + strand at 3244.945 kb on + strand at 3245.009 kb on + strand, within BWI76_RS15490 at 3245.047 kb on - strand, within BWI76_RS15490 at 3245.065 kb on + strand, within BWI76_RS15490 at 3245.440 kb on + strand, within BWI76_RS15490
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,243,241 - -0.7 3,243,323 + +1.4 3,243,475 + BWI76_RS15480 0.12 -0.2 3,243,631 - BWI76_RS15480 0.35 -0.5 3,243,646 - BWI76_RS15480 0.37 -1.6 3,243,752 + BWI76_RS15480 0.52 +0.4 3,243,776 - BWI76_RS15480 0.56 +2.3 3,243,846 + BWI76_RS15480 0.66 -1.1 3,243,846 + BWI76_RS15480 0.66 -0.8 3,243,895 + BWI76_RS15480 0.73 -1.8 3,243,895 + BWI76_RS15480 0.73 -2.9 3,243,919 - BWI76_RS15480 0.77 -0.4 3,243,926 - BWI76_RS15480 0.78 -0.8 3,243,976 + BWI76_RS15480 0.85 -0.0 3,243,978 - BWI76_RS15480 0.85 +1.7 3,243,985 - BWI76_RS15480 0.86 -0.3 3,243,985 - BWI76_RS15480 0.86 -3.4 3,244,003 + BWI76_RS15480 0.89 +1.9 3,244,011 - -2.7 3,244,011 - +0.5 3,244,011 - -1.1 3,244,011 - -0.2 3,244,094 + -2.2 3,244,124 - -0.8 3,244,212 - BWI76_RS15485 0.19 -1.5 3,244,276 - BWI76_RS15485 0.34 +1.0 3,244,320 - BWI76_RS15485 0.44 -1.6 3,244,332 - BWI76_RS15485 0.47 -1.7 3,244,338 + BWI76_RS15485 0.48 +1.4 3,244,346 - BWI76_RS15485 0.50 -0.4 3,244,346 - BWI76_RS15485 0.50 -0.1 3,244,357 + BWI76_RS15485 0.52 -0.6 3,244,382 - BWI76_RS15485 0.58 -3.9 3,244,382 - BWI76_RS15485 0.58 -0.4 3,244,439 + BWI76_RS15485 0.71 -1.2 3,244,452 - BWI76_RS15485 0.74 +0.6 3,244,452 - BWI76_RS15485 0.74 -3.1 3,244,452 - BWI76_RS15485 0.74 -1.9 3,244,452 - BWI76_RS15485 0.74 -3.7 3,244,494 + BWI76_RS15485 0.84 -0.2 3,244,520 - -0.3 3,244,540 - -0.2 3,244,540 - -1.9 3,244,853 + -1.9 3,244,853 + +2.4 3,244,854 + -1.8 3,244,861 - -0.9 3,244,862 - +0.5 3,244,873 + -1.0 3,244,873 + +1.6 3,244,873 + -1.1 3,244,873 + +1.4 3,244,881 - -1.2 3,244,881 - -0.7 3,244,881 - +1.5 3,244,913 - -0.4 3,244,935 + -0.2 3,244,940 + -0.2 3,244,945 + +0.0 3,245,009 + BWI76_RS15490 0.11 +0.9 3,245,047 - BWI76_RS15490 0.14 -0.4 3,245,065 + BWI76_RS15490 0.15 -3.8 3,245,440 + BWI76_RS15490 0.41 +1.2
Or see this region's nucleotide sequence