Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09995 and BWI76_RS10000 are separated by 28 nucleotides BWI76_RS10000 and BWI76_RS10005 are separated by 154 nucleotides
BWI76_RS09995: BWI76_RS09995 - DNA helicase IV, at 2,103,951 to 2,106,005
_RS09995
BWI76_RS10000: BWI76_RS10000 - methylglyoxal synthase, at 2,106,034 to 2,106,492
_RS10000
BWI76_RS10005: BWI76_RS10005 - CoA-binding protein, at 2,106,647 to 2,107,060
_RS10005
Position (kb)
2106
2107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2105.437 kb on - strand, within BWI76_RS09995 at 2105.444 kb on + strand, within BWI76_RS09995 at 2105.444 kb on + strand, within BWI76_RS09995 at 2105.534 kb on - strand, within BWI76_RS09995 at 2105.879 kb on - strand at 2105.910 kb on + strand at 2105.910 kb on + strand at 2105.918 kb on - strand at 2105.918 kb on - strand at 2106.056 kb on - strand at 2106.128 kb on + strand, within BWI76_RS10000 at 2106.128 kb on + strand, within BWI76_RS10000 at 2106.197 kb on - strand, within BWI76_RS10000 at 2106.197 kb on - strand, within BWI76_RS10000 at 2106.269 kb on + strand, within BWI76_RS10000 at 2106.277 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.323 kb on - strand, within BWI76_RS10000 at 2106.447 kb on + strand at 2106.455 kb on - strand at 2106.524 kb on - strand at 2106.582 kb on + strand at 2106.584 kb on - strand at 2106.626 kb on - strand at 2106.708 kb on - strand, within BWI76_RS10005 at 2106.708 kb on - strand, within BWI76_RS10005 at 2106.720 kb on - strand, within BWI76_RS10005 at 2106.772 kb on + strand, within BWI76_RS10005 at 2106.801 kb on + strand, within BWI76_RS10005 at 2106.804 kb on + strand, within BWI76_RS10005 at 2106.817 kb on - strand, within BWI76_RS10005 at 2106.882 kb on + strand, within BWI76_RS10005 at 2106.905 kb on + strand, within BWI76_RS10005 at 2106.949 kb on + strand, within BWI76_RS10005 at 2106.985 kb on - strand, within BWI76_RS10005 at 2106.998 kb on - strand, within BWI76_RS10005 at 2107.007 kb on + strand, within BWI76_RS10005 at 2107.042 kb on + strand at 2107.046 kb on + strand at 2107.061 kb on + strand at 2107.061 kb on + strand at 2107.090 kb on - strand at 2107.091 kb on - strand at 2107.091 kb on - strand at 2107.188 kb on - strand at 2107.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,105,437 - BWI76_RS09995 0.72 +0.0 2,105,444 + BWI76_RS09995 0.73 +0.2 2,105,444 + BWI76_RS09995 0.73 +0.5 2,105,534 - BWI76_RS09995 0.77 +1.6 2,105,879 - -0.6 2,105,910 + -1.9 2,105,910 + +0.1 2,105,918 - -2.2 2,105,918 - -0.9 2,106,056 - +0.2 2,106,128 + BWI76_RS10000 0.20 -1.0 2,106,128 + BWI76_RS10000 0.20 -0.6 2,106,197 - BWI76_RS10000 0.36 -1.3 2,106,197 - BWI76_RS10000 0.36 -1.3 2,106,269 + BWI76_RS10000 0.51 +0.2 2,106,277 - BWI76_RS10000 0.53 -0.4 2,106,323 - BWI76_RS10000 0.63 -2.9 2,106,323 - BWI76_RS10000 0.63 -0.3 2,106,323 - BWI76_RS10000 0.63 -0.4 2,106,447 + -1.6 2,106,455 - -1.7 2,106,524 - -2.1 2,106,582 + +0.7 2,106,584 - -0.9 2,106,626 - -1.7 2,106,708 - BWI76_RS10005 0.15 -0.4 2,106,708 - BWI76_RS10005 0.15 -0.4 2,106,720 - BWI76_RS10005 0.18 -1.8 2,106,772 + BWI76_RS10005 0.30 +1.5 2,106,801 + BWI76_RS10005 0.37 +1.0 2,106,804 + BWI76_RS10005 0.38 +0.1 2,106,817 - BWI76_RS10005 0.41 -0.9 2,106,882 + BWI76_RS10005 0.57 -0.6 2,106,905 + BWI76_RS10005 0.62 -0.3 2,106,949 + BWI76_RS10005 0.73 +1.2 2,106,985 - BWI76_RS10005 0.82 -1.7 2,106,998 - BWI76_RS10005 0.85 -1.1 2,107,007 + BWI76_RS10005 0.87 +0.4 2,107,042 + +1.0 2,107,046 + +1.2 2,107,061 + +0.3 2,107,061 + +0.6 2,107,090 - -0.1 2,107,091 - -0.6 2,107,091 - -1.6 2,107,188 - +2.4 2,107,196 - +1.3
Or see this region's nucleotide sequence