Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04400 and BWI76_RS04405 are separated by 44 nucleotides BWI76_RS04405 and BWI76_RS04410 overlap by 8 nucleotides BWI76_RS04410 and BWI76_RS04415 are separated by 246 nucleotides
BWI76_RS04400: BWI76_RS04400 - alanyl-tRNA synthetase, at 907,968 to 908,597
_RS04400
BWI76_RS04405: BWI76_RS04405 - response regulator, at 908,642 to 909,340
_RS04405
BWI76_RS04410: BWI76_RS04410 - signal transduction histidine kinase regulating citrate/malate metabolism, at 909,333 to 910,961
_RS04410
BWI76_RS04415: BWI76_RS04415 - oxaloacetate decarboxylase subunit beta, at 911,208 to 912,509
_RS04415
Position (kb) 909
910
911 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 908.336 kb on - strand, within BWI76_RS04400 at 908.365 kb on - strand, within BWI76_RS04400 at 908.497 kb on - strand, within BWI76_RS04400 at 908.509 kb on + strand, within BWI76_RS04400 at 908.595 kb on - strand at 908.740 kb on + strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.836 kb on - strand, within BWI76_RS04405 at 908.843 kb on + strand, within BWI76_RS04405 at 908.850 kb on - strand, within BWI76_RS04405 at 908.855 kb on + strand, within BWI76_RS04405 at 908.910 kb on + strand, within BWI76_RS04405 at 908.956 kb on + strand, within BWI76_RS04405 at 909.112 kb on + strand, within BWI76_RS04405 at 909.170 kb on - strand, within BWI76_RS04405 at 909.186 kb on + strand, within BWI76_RS04405 at 909.204 kb on - strand, within BWI76_RS04405 at 909.238 kb on - strand, within BWI76_RS04405 at 909.257 kb on - strand, within BWI76_RS04405 at 909.273 kb on + strand at 909.298 kb on + strand at 909.306 kb on - strand at 909.325 kb on + strand at 909.333 kb on - strand at 909.447 kb on - strand at 909.449 kb on + strand at 909.599 kb on + strand, within BWI76_RS04410 at 909.607 kb on - strand, within BWI76_RS04410 at 909.699 kb on + strand, within BWI76_RS04410 at 909.700 kb on - strand, within BWI76_RS04410 at 909.707 kb on - strand, within BWI76_RS04410 at 909.708 kb on - strand, within BWI76_RS04410 at 909.731 kb on + strand, within BWI76_RS04410 at 909.739 kb on - strand, within BWI76_RS04410 at 909.754 kb on + strand, within BWI76_RS04410 at 909.832 kb on - strand, within BWI76_RS04410 at 909.843 kb on - strand, within BWI76_RS04410 at 910.034 kb on - strand, within BWI76_RS04410 at 910.034 kb on - strand, within BWI76_RS04410 at 910.080 kb on - strand, within BWI76_RS04410 at 910.082 kb on + strand, within BWI76_RS04410 at 910.082 kb on + strand, within BWI76_RS04410 at 910.090 kb on - strand, within BWI76_RS04410 at 910.207 kb on - strand, within BWI76_RS04410 at 910.313 kb on + strand, within BWI76_RS04410 at 910.380 kb on + strand, within BWI76_RS04410 at 910.472 kb on + strand, within BWI76_RS04410 at 910.532 kb on + strand, within BWI76_RS04410 at 910.532 kb on + strand, within BWI76_RS04410 at 910.584 kb on - strand, within BWI76_RS04410 at 910.658 kb on - strand, within BWI76_RS04410
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 908,336 - BWI76_RS04400 0.58 +0.5 908,365 - BWI76_RS04400 0.63 -1.0 908,497 - BWI76_RS04400 0.84 +0.8 908,509 + BWI76_RS04400 0.86 +0.9 908,595 - -0.4 908,740 + BWI76_RS04405 0.14 +0.2 908,753 - BWI76_RS04405 0.16 +1.5 908,753 - BWI76_RS04405 0.16 -1.2 908,836 - BWI76_RS04405 0.28 +2.2 908,843 + BWI76_RS04405 0.29 +1.4 908,850 - BWI76_RS04405 0.30 -0.7 908,855 + BWI76_RS04405 0.30 -0.2 908,910 + BWI76_RS04405 0.38 -0.6 908,956 + BWI76_RS04405 0.45 -0.1 909,112 + BWI76_RS04405 0.67 +0.9 909,170 - BWI76_RS04405 0.76 -0.7 909,186 + BWI76_RS04405 0.78 -0.6 909,204 - BWI76_RS04405 0.80 -0.3 909,238 - BWI76_RS04405 0.85 -1.6 909,257 - BWI76_RS04405 0.88 -0.4 909,273 + +0.9 909,298 + +0.5 909,306 - -2.5 909,325 + -0.8 909,333 - -0.4 909,447 - -0.4 909,449 + -2.1 909,599 + BWI76_RS04410 0.16 +0.9 909,607 - BWI76_RS04410 0.17 -1.0 909,699 + BWI76_RS04410 0.22 -1.8 909,700 - BWI76_RS04410 0.23 -0.5 909,707 - BWI76_RS04410 0.23 -2.0 909,708 - BWI76_RS04410 0.23 +0.8 909,731 + BWI76_RS04410 0.24 +1.2 909,739 - BWI76_RS04410 0.25 -2.5 909,754 + BWI76_RS04410 0.26 +0.7 909,832 - BWI76_RS04410 0.31 +0.1 909,843 - BWI76_RS04410 0.31 -0.2 910,034 - BWI76_RS04410 0.43 -3.0 910,034 - BWI76_RS04410 0.43 -1.0 910,080 - BWI76_RS04410 0.46 -3.0 910,082 + BWI76_RS04410 0.46 -1.4 910,082 + BWI76_RS04410 0.46 +1.4 910,090 - BWI76_RS04410 0.46 +0.9 910,207 - BWI76_RS04410 0.54 -0.6 910,313 + BWI76_RS04410 0.60 -2.5 910,380 + BWI76_RS04410 0.64 -0.4 910,472 + BWI76_RS04410 0.70 -1.8 910,532 + BWI76_RS04410 0.74 +0.1 910,532 + BWI76_RS04410 0.74 -1.6 910,584 - BWI76_RS04410 0.77 -1.1 910,658 - BWI76_RS04410 0.81 -0.6
Or see this region's nucleotide sequence