Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03315 and BWI76_RS03320 are separated by 56 nucleotides BWI76_RS03320 and BWI76_RS03325 are separated by 196 nucleotides
BWI76_RS03315: BWI76_RS03315 - clavaldehyde dehydrogenase, at 683,133 to 683,900
_RS03315
BWI76_RS03320: BWI76_RS03320 - alcohol dehydrogenase, at 683,957 to 685,015
_RS03320
BWI76_RS03325: BWI76_RS03325 - LysR family transcriptional regulator, at 685,212 to 686,105
_RS03325
Position (kb)
683
684
685
686 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 683.035 kb on + strand at 683.180 kb on - strand at 683.424 kb on - strand, within BWI76_RS03315 at 683.497 kb on + strand at 683.548 kb on + strand, within BWI76_RS03315 at 683.554 kb on + strand, within BWI76_RS03315 at 683.562 kb on - strand, within BWI76_RS03315 at 683.562 kb on - strand, within BWI76_RS03315 at 683.844 kb on + strand at 683.934 kb on + strand at 683.947 kb on + strand at 683.953 kb on - strand at 683.965 kb on - strand at 683.981 kb on - strand at 684.000 kb on - strand at 684.000 kb on - strand at 684.086 kb on - strand, within BWI76_RS03320 at 684.104 kb on + strand, within BWI76_RS03320 at 684.114 kb on + strand, within BWI76_RS03320 at 684.130 kb on + strand, within BWI76_RS03320 at 684.159 kb on + strand, within BWI76_RS03320 at 684.196 kb on + strand, within BWI76_RS03320 at 684.196 kb on + strand, within BWI76_RS03320 at 684.396 kb on + strand at 684.431 kb on + strand, within BWI76_RS03320 at 684.461 kb on + strand, within BWI76_RS03320 at 684.492 kb on + strand, within BWI76_RS03320 at 684.510 kb on + strand, within BWI76_RS03320 at 684.519 kb on - strand, within BWI76_RS03320 at 684.546 kb on + strand, within BWI76_RS03320 at 684.566 kb on - strand, within BWI76_RS03320 at 684.589 kb on + strand, within BWI76_RS03320 at 684.597 kb on + strand, within BWI76_RS03320 at 684.606 kb on - strand, within BWI76_RS03320 at 684.609 kb on + strand, within BWI76_RS03320 at 684.708 kb on + strand, within BWI76_RS03320 at 684.712 kb on + strand, within BWI76_RS03320 at 684.752 kb on + strand, within BWI76_RS03320 at 684.827 kb on + strand, within BWI76_RS03320 at 684.833 kb on - strand, within BWI76_RS03320 at 684.960 kb on - strand at 684.963 kb on + strand at 684.986 kb on + strand at 685.040 kb on - strand at 685.040 kb on - strand at 685.056 kb on + strand at 685.056 kb on + strand at 685.064 kb on + strand at 685.064 kb on - strand at 685.064 kb on - strand at 685.097 kb on - strand at 685.161 kb on - strand at 685.170 kb on + strand at 685.178 kb on - strand at 685.187 kb on + strand at 685.222 kb on + strand at 685.225 kb on + strand at 685.225 kb on + strand at 685.232 kb on + strand at 685.233 kb on - strand at 685.233 kb on - strand at 685.243 kb on + strand at 685.243 kb on - strand at 685.249 kb on - strand at 685.289 kb on + strand at 685.305 kb on + strand, within BWI76_RS03325 at 685.305 kb on + strand, within BWI76_RS03325 at 685.305 kb on + strand, within BWI76_RS03325 at 685.313 kb on - strand, within BWI76_RS03325 at 685.320 kb on + strand at 685.336 kb on - strand, within BWI76_RS03325 at 685.354 kb on + strand, within BWI76_RS03325 at 685.363 kb on + strand, within BWI76_RS03325 at 685.371 kb on - strand, within BWI76_RS03325 at 685.371 kb on - strand, within BWI76_RS03325 at 685.373 kb on + strand, within BWI76_RS03325 at 685.420 kb on + strand, within BWI76_RS03325 at 685.430 kb on + strand, within BWI76_RS03325 at 685.430 kb on + strand, within BWI76_RS03325 at 685.438 kb on - strand, within BWI76_RS03325 at 685.465 kb on + strand, within BWI76_RS03325 at 685.472 kb on + strand, within BWI76_RS03325 at 685.481 kb on + strand, within BWI76_RS03325 at 685.488 kb on + strand, within BWI76_RS03325 at 685.489 kb on - strand, within BWI76_RS03325 at 685.489 kb on - strand, within BWI76_RS03325 at 685.586 kb on + strand, within BWI76_RS03325 at 685.690 kb on + strand, within BWI76_RS03325 at 685.952 kb on + strand, within BWI76_RS03325 at 685.952 kb on + strand, within BWI76_RS03325 at 685.952 kb on + strand, within BWI76_RS03325 at 685.960 kb on - strand, within BWI76_RS03325 at 685.960 kb on - strand, within BWI76_RS03325 at 685.960 kb on - strand, within BWI76_RS03325 at 685.960 kb on - strand, within BWI76_RS03325
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 683,035 + +0.7 683,180 - -0.1 683,424 - BWI76_RS03315 0.38 +0.5 683,497 + -0.8 683,548 + BWI76_RS03315 0.54 +0.2 683,554 + BWI76_RS03315 0.55 -0.3 683,562 - BWI76_RS03315 0.56 +0.1 683,562 - BWI76_RS03315 0.56 +0.3 683,844 + -0.7 683,934 + -1.2 683,947 + +1.3 683,953 - +0.9 683,965 - +1.4 683,981 - -1.3 684,000 - -1.6 684,000 - +0.7 684,086 - BWI76_RS03320 0.12 -1.2 684,104 + BWI76_RS03320 0.14 -1.7 684,114 + BWI76_RS03320 0.15 +0.4 684,130 + BWI76_RS03320 0.16 -0.8 684,159 + BWI76_RS03320 0.19 -0.6 684,196 + BWI76_RS03320 0.23 -1.6 684,196 + BWI76_RS03320 0.23 +1.4 684,396 + -0.2 684,431 + BWI76_RS03320 0.45 -0.1 684,461 + BWI76_RS03320 0.48 -1.4 684,492 + BWI76_RS03320 0.51 -1.2 684,510 + BWI76_RS03320 0.52 +1.0 684,519 - BWI76_RS03320 0.53 -0.1 684,546 + BWI76_RS03320 0.56 +0.6 684,566 - BWI76_RS03320 0.58 -1.0 684,589 + BWI76_RS03320 0.60 +0.2 684,597 + BWI76_RS03320 0.60 +0.0 684,606 - BWI76_RS03320 0.61 -0.8 684,609 + BWI76_RS03320 0.62 -1.3 684,708 + BWI76_RS03320 0.71 +1.8 684,712 + BWI76_RS03320 0.71 -2.0 684,752 + BWI76_RS03320 0.75 -1.0 684,827 + BWI76_RS03320 0.82 -4.2 684,833 - BWI76_RS03320 0.83 -1.3 684,960 - +1.3 684,963 + -4.6 684,986 + -0.7 685,040 - +2.2 685,040 - -0.1 685,056 + +0.2 685,056 + -0.0 685,064 + -2.4 685,064 - +2.6 685,064 - -0.2 685,097 - +1.2 685,161 - -0.5 685,170 + -2.1 685,178 - +2.4 685,187 + +0.1 685,222 + +0.5 685,225 + -4.6 685,225 + -0.4 685,232 + +1.3 685,233 - -0.2 685,233 - +4.2 685,243 + +0.7 685,243 - -0.1 685,249 - +0.3 685,289 + +1.0 685,305 + BWI76_RS03325 0.10 -1.3 685,305 + BWI76_RS03325 0.10 -0.5 685,305 + BWI76_RS03325 0.10 +0.2 685,313 - BWI76_RS03325 0.11 +2.2 685,320 + +1.6 685,336 - BWI76_RS03325 0.14 -0.8 685,354 + BWI76_RS03325 0.16 +0.0 685,363 + BWI76_RS03325 0.17 +0.4 685,371 - BWI76_RS03325 0.18 +0.5 685,371 - BWI76_RS03325 0.18 +0.3 685,373 + BWI76_RS03325 0.18 -2.4 685,420 + BWI76_RS03325 0.23 -0.6 685,430 + BWI76_RS03325 0.24 -0.8 685,430 + BWI76_RS03325 0.24 -0.9 685,438 - BWI76_RS03325 0.25 +0.3 685,465 + BWI76_RS03325 0.28 +4.8 685,472 + BWI76_RS03325 0.29 +0.4 685,481 + BWI76_RS03325 0.30 -1.5 685,488 + BWI76_RS03325 0.31 +0.5 685,489 - BWI76_RS03325 0.31 -1.9 685,489 - BWI76_RS03325 0.31 -0.1 685,586 + BWI76_RS03325 0.42 -1.1 685,690 + BWI76_RS03325 0.53 -0.3 685,952 + BWI76_RS03325 0.83 -0.1 685,952 + BWI76_RS03325 0.83 -3.1 685,952 + BWI76_RS03325 0.83 +0.2 685,960 - BWI76_RS03325 0.84 -1.9 685,960 - BWI76_RS03325 0.84 -1.6 685,960 - BWI76_RS03325 0.84 -0.4 685,960 - BWI76_RS03325 0.84 +0.9
Or see this region's nucleotide sequence