Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02710

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02700 and BWI76_RS02705 are separated by 115 nucleotidesBWI76_RS02705 and BWI76_RS02710 are separated by 82 nucleotidesBWI76_RS02710 and BWI76_RS02715 are separated by 75 nucleotidesBWI76_RS02715 and BWI76_RS02720 are separated by 80 nucleotides BWI76_RS02700: BWI76_RS02700 - D-serine/D-alanine/glycine transporter, at 546,870 to 548,273 _RS02700 BWI76_RS02705: BWI76_RS02705 - iron-sulfur cluster repair di-iron protein, at 548,389 to 549,051 _RS02705 BWI76_RS02710: BWI76_RS02710 - EamA family transporter, at 549,134 to 550,105 _RS02710 BWI76_RS02715: BWI76_RS02715 - putative helix-turn-helix AraC-type transcriptional regulator, at 550,181 to 551,005 _RS02715 BWI76_RS02720: BWI76_RS02720 - NAD(P)-dependent oxidoreductase, at 551,086 to 551,934 _RS02720 Position (kb) 549 550 551Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 548.215 kb on + strandat 548.218 kb on - strandat 548.258 kb on - strandat 548.408 kb on + strandat 548.408 kb on + strandat 548.519 kb on + strand, within BWI76_RS02705at 548.554 kb on - strand, within BWI76_RS02705at 548.615 kb on + strand, within BWI76_RS02705at 548.615 kb on + strand, within BWI76_RS02705at 548.684 kb on - strand, within BWI76_RS02705at 548.701 kb on - strand, within BWI76_RS02705at 549.182 kb on + strandat 549.280 kb on - strand, within BWI76_RS02710at 549.395 kb on + strand, within BWI76_RS02710at 549.408 kb on - strand, within BWI76_RS02710at 549.432 kb on - strand, within BWI76_RS02710at 549.573 kb on + strand, within BWI76_RS02710at 549.583 kb on + strand, within BWI76_RS02710at 549.591 kb on - strand, within BWI76_RS02710at 549.591 kb on - strand, within BWI76_RS02710at 549.602 kb on - strand, within BWI76_RS02710at 549.711 kb on + strand, within BWI76_RS02710at 549.716 kb on + strand, within BWI76_RS02710at 549.857 kb on + strand, within BWI76_RS02710at 549.857 kb on + strand, within BWI76_RS02710at 549.857 kb on + strand, within BWI76_RS02710at 549.959 kb on + strand, within BWI76_RS02710at 550.138 kb on + strandat 550.138 kb on + strandat 550.224 kb on - strandat 550.235 kb on + strandat 550.247 kb on + strandat 550.255 kb on - strandat 550.255 kb on - strandat 550.521 kb on - strand, within BWI76_RS02715at 550.654 kb on + strand, within BWI76_RS02715at 550.761 kb on + strand, within BWI76_RS02715at 550.789 kb on + strand, within BWI76_RS02715at 550.818 kb on - strand, within BWI76_RS02715at 550.881 kb on - strand, within BWI76_RS02715at 550.896 kb on - strand, within BWI76_RS02715at 550.896 kb on - strand, within BWI76_RS02715at 550.985 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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548,215 + -1.1
548,218 - -1.2
548,258 - +0.2
548,408 + +1.2
548,408 + -0.6
548,519 + BWI76_RS02705 0.20 +0.2
548,554 - BWI76_RS02705 0.25 +0.2
548,615 + BWI76_RS02705 0.34 +0.4
548,615 + BWI76_RS02705 0.34 +0.8
548,684 - BWI76_RS02705 0.44 +0.3
548,701 - BWI76_RS02705 0.47 +0.2
549,182 + -0.2
549,280 - BWI76_RS02710 0.15 +0.3
549,395 + BWI76_RS02710 0.27 -0.5
549,408 - BWI76_RS02710 0.28 +0.2
549,432 - BWI76_RS02710 0.31 -1.6
549,573 + BWI76_RS02710 0.45 +2.2
549,583 + BWI76_RS02710 0.46 -0.5
549,591 - BWI76_RS02710 0.47 +1.6
549,591 - BWI76_RS02710 0.47 +0.5
549,602 - BWI76_RS02710 0.48 +3.6
549,711 + BWI76_RS02710 0.59 +2.0
549,716 + BWI76_RS02710 0.60 -0.6
549,857 + BWI76_RS02710 0.74 +0.2
549,857 + BWI76_RS02710 0.74 +0.9
549,857 + BWI76_RS02710 0.74 -2.8
549,959 + BWI76_RS02710 0.85 -1.3
550,138 + -0.6
550,138 + -0.9
550,224 - -0.1
550,235 + -3.8
550,247 + +0.6
550,255 - +1.9
550,255 - +1.6
550,521 - BWI76_RS02715 0.41 -0.7
550,654 + BWI76_RS02715 0.57 -0.2
550,761 + BWI76_RS02715 0.70 -0.2
550,789 + BWI76_RS02715 0.74 -0.0
550,818 - BWI76_RS02715 0.77 -1.9
550,881 - BWI76_RS02715 0.85 -1.0
550,896 - BWI76_RS02715 0.87 +0.2
550,896 - BWI76_RS02715 0.87 +0.6
550,985 - +0.8

Or see this region's nucleotide sequence