Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02620 and BWI76_RS02625 are separated by 12 nucleotides BWI76_RS02625 and BWI76_RS02630 are separated by 9 nucleotides BWI76_RS02630 and BWI76_RS02635 are separated by 13 nucleotides BWI76_RS02635 and BWI76_RS02640 are separated by 9 nucleotides
BWI76_RS02620: BWI76_RS02620 - PTS ascorbate transporter subunit IIC, at 536,057 to 537,457
_RS02620
BWI76_RS02625: BWI76_RS02625 - PTS ascorbate transporter subunit IIB, at 537,470 to 537,775
_RS02625
BWI76_RS02630: BWI76_RS02630 - PTS ascorbate transporter subunit IIA, at 537,785 to 538,252
_RS02630
BWI76_RS02635: BWI76_RS02635 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 538,266 to 538,916
_RS02635
BWI76_RS02640: BWI76_RS02640 - xylulose 5-phosphate 3-epimerase, at 538,926 to 539,780
_RS02640
Position (kb) 537
538
539 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 536.827 kb on + strand, within BWI76_RS02620 at 536.836 kb on + strand, within BWI76_RS02620 at 536.844 kb on - strand, within BWI76_RS02620 at 536.856 kb on + strand, within BWI76_RS02620 at 536.905 kb on + strand, within BWI76_RS02620 at 537.035 kb on + strand, within BWI76_RS02620 at 537.115 kb on - strand, within BWI76_RS02620 at 537.241 kb on + strand, within BWI76_RS02620 at 537.331 kb on - strand at 537.376 kb on + strand at 537.397 kb on + strand at 537.487 kb on + strand at 537.491 kb on + strand at 537.529 kb on + strand, within BWI76_RS02625 at 537.583 kb on - strand, within BWI76_RS02625 at 537.590 kb on - strand, within BWI76_RS02625 at 537.644 kb on - strand, within BWI76_RS02625 at 537.824 kb on - strand at 537.868 kb on + strand, within BWI76_RS02630 at 537.908 kb on + strand, within BWI76_RS02630 at 537.941 kb on - strand, within BWI76_RS02630 at 538.020 kb on + strand, within BWI76_RS02630 at 538.154 kb on - strand, within BWI76_RS02630 at 538.156 kb on + strand, within BWI76_RS02630 at 538.168 kb on + strand, within BWI76_RS02630 at 538.334 kb on + strand, within BWI76_RS02635 at 538.342 kb on - strand, within BWI76_RS02635 at 538.538 kb on + strand, within BWI76_RS02635 at 538.707 kb on - strand, within BWI76_RS02635 at 538.731 kb on + strand, within BWI76_RS02635 at 538.810 kb on - strand, within BWI76_RS02635 at 538.883 kb on + strand at 538.891 kb on - strand at 538.904 kb on + strand at 538.980 kb on - strand at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.113 kb on - strand, within BWI76_RS02640 at 539.187 kb on - strand, within BWI76_RS02640
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 536,827 + BWI76_RS02620 0.55 -1.6 536,836 + BWI76_RS02620 0.56 -1.0 536,844 - BWI76_RS02620 0.56 -0.5 536,856 + BWI76_RS02620 0.57 -0.4 536,905 + BWI76_RS02620 0.61 -0.5 537,035 + BWI76_RS02620 0.70 -1.3 537,115 - BWI76_RS02620 0.76 +0.4 537,241 + BWI76_RS02620 0.85 -0.7 537,331 - -0.9 537,376 + -0.0 537,397 + +4.8 537,487 + +0.3 537,491 + -0.2 537,529 + BWI76_RS02625 0.19 +0.2 537,583 - BWI76_RS02625 0.37 -2.0 537,590 - BWI76_RS02625 0.39 -0.6 537,644 - BWI76_RS02625 0.57 -1.4 537,824 - -0.9 537,868 + BWI76_RS02630 0.18 -0.1 537,908 + BWI76_RS02630 0.26 -0.3 537,941 - BWI76_RS02630 0.33 -0.4 538,020 + BWI76_RS02630 0.50 -2.2 538,154 - BWI76_RS02630 0.79 -1.6 538,156 + BWI76_RS02630 0.79 -0.4 538,168 + BWI76_RS02630 0.82 -0.8 538,334 + BWI76_RS02635 0.10 +0.1 538,342 - BWI76_RS02635 0.12 +0.7 538,538 + BWI76_RS02635 0.42 +0.1 538,707 - BWI76_RS02635 0.68 -1.6 538,731 + BWI76_RS02635 0.71 -0.2 538,810 - BWI76_RS02635 0.84 -2.8 538,883 + -0.2 538,891 - +0.1 538,904 + -1.5 538,980 - -2.5 539,103 - BWI76_RS02640 0.21 +0.3 539,103 - BWI76_RS02640 0.21 -1.3 539,103 - BWI76_RS02640 0.21 -1.1 539,113 - BWI76_RS02640 0.22 -1.4 539,187 - BWI76_RS02640 0.31 +0.6
Or see this region's nucleotide sequence