Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06175

Experiment: Taped volatile agar plate with Trichoderma atroviridae IMI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06165 and BWI76_RS06170 are separated by 46 nucleotidesBWI76_RS06170 and BWI76_RS06175 are separated by 116 nucleotidesBWI76_RS06175 and BWI76_RS06180 overlap by 4 nucleotides BWI76_RS06165: BWI76_RS06165 - phosphate starvation-inducible protein PsiF, at 1,295,596 to 1,295,916 _RS06165 BWI76_RS06170: BWI76_RS06170 - pyrroline-5-carboxylate reductase, at 1,295,963 to 1,296,772 _RS06170 BWI76_RS06175: BWI76_RS06175 - DUF188 domain-containing protein, at 1,296,889 to 1,297,347 _RS06175 BWI76_RS06180: BWI76_RS06180 - hypothetical protein, at 1,297,344 to 1,298,042 _RS06180 Position (kb) 1296 1297 1298Strain fitness (log2 ratio) -1 0 1at 1296.958 kb on - strand, within BWI76_RS06175at 1297.039 kb on + strand, within BWI76_RS06175at 1297.173 kb on + strand, within BWI76_RS06175at 1297.405 kb on + strandat 1297.546 kb on - strand, within BWI76_RS06180at 1297.580 kb on - strand, within BWI76_RS06180at 1297.853 kb on + strand, within BWI76_RS06180at 1298.038 kb on - strandat 1298.039 kb on + strandat 1298.052 kb on - strandat 1298.125 kb on - strandat 1298.125 kb on - strandat 1298.125 kb on - strandat 1298.321 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Taped volatile agar plate with Trichoderma atroviridae IMI
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1,296,958 - BWI76_RS06175 0.15 -1.4
1,297,039 + BWI76_RS06175 0.33 -1.0
1,297,173 + BWI76_RS06175 0.62 -1.1
1,297,405 + +0.7
1,297,546 - BWI76_RS06180 0.29 +0.5
1,297,580 - BWI76_RS06180 0.34 +0.4
1,297,853 + BWI76_RS06180 0.73 -0.3
1,298,038 - +1.7
1,298,039 + +0.2
1,298,052 - -0.4
1,298,125 - -0.5
1,298,125 - +0.2
1,298,125 - +0.6
1,298,321 - -0.9

Or see this region's nucleotide sequence