Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt BWI76_RS15120 and BWI76_RS15125 overlap by 7 nucleotides BWI76_RS15125 and BWI76_RS15130 overlap by 4 nucleotides BWI76_RS15130 and BWI76_RS15135 are separated by 300 nucleotides BWI76_RS15135 and BWI76_RS15140 are separated by 35 nucleotides  
        
        BWI76_RS15120: BWI76_RS15120 - hypothetical protein, at 3,170,405 to 3,170,623 
         
        _RS15120 
         
        
        BWI76_RS15125: BWI76_RS15125 - acetyltransferase, at 3,170,617 to 3,171,195 
         
        _RS15125 
         
        
        BWI76_RS15130: BWI76_RS15130 - hypothetical protein, at 3,171,192 to 3,171,638 
         
        _RS15130 
         
        
        BWI76_RS15135: BWI76_RS15135 - hypothetical protein, at 3,171,939 to 3,172,229 
         
        _RS15135 
         
        
        BWI76_RS15140: BWI76_RS15140 - CusA/CzcA family heavy metal efflux RND transporter, at 3,172,265 to 3,175,378 
         
        _RS15140 
         Position (kb)  
3171 
 
3172 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 at 3170.213 kb on + strand at 3170.300 kb on + strand at 3170.346 kb on + strand at 3170.402 kb on + strand at 3170.402 kb on + strand at 3170.402 kb on + strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.418 kb on - strand at 3170.422 kb on + strand at 3170.422 kb on + strand at 3170.423 kb on - strand at 3170.430 kb on - strand, within BWI76_RS15120 at 3170.439 kb on - strand, within BWI76_RS15120 at 3170.445 kb on - strand at 3170.496 kb on - strand, within BWI76_RS15120 at 3170.511 kb on + strand, within BWI76_RS15120 at 3170.519 kb on - strand at 3170.548 kb on - strand, within BWI76_RS15120 at 3170.625 kb on + strand at 3170.633 kb on - strand at 3170.655 kb on - strand at 3170.707 kb on - strand, within BWI76_RS15125 at 3170.814 kb on - strand, within BWI76_RS15125 at 3170.822 kb on - strand, within BWI76_RS15125 at 3170.880 kb on + strand, within BWI76_RS15125 at 3170.991 kb on + strand, within BWI76_RS15125 at 3171.183 kb on - strand at 3171.359 kb on + strand, within BWI76_RS15130 at 3171.413 kb on - strand, within BWI76_RS15130 at 3171.745 kb on - strand at 3171.904 kb on - strand at 3172.060 kb on - strand, within BWI76_RS15135 at 3172.090 kb on - strand, within BWI76_RS15135 at 3172.159 kb on + strand, within BWI76_RS15135 at 3172.167 kb on - strand, within BWI76_RS15135 at 3172.346 kb on + strand at 3172.542 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Parafilmed volatile agar plate with Trichoderma atroviridae IMI             remove   3,170,213   +        -0.3   3,170,300   +        +0.5   3,170,346   +        -1.0   3,170,402   +        +1.0   3,170,402   +        -1.1   3,170,402   +        +0.6   3,170,410   -        -2.2   3,170,410   -        -2.7   3,170,410   -        -0.1   3,170,410   -        -1.0   3,170,410   -        -1.8   3,170,418   -        -1.5   3,170,422   +        -1.6   3,170,422   +        +0.5   3,170,423   -        -2.8   3,170,430   -    BWI76_RS15120   0.11  -1.2   3,170,439   -    BWI76_RS15120   0.16  -0.1   3,170,445   -        +1.7   3,170,496   -    BWI76_RS15120   0.42  +0.7   3,170,511   +    BWI76_RS15120   0.48  -3.4   3,170,519   -        -0.7   3,170,548   -    BWI76_RS15120   0.65  +0.5   3,170,625   +        +0.2   3,170,633   -        +1.9   3,170,655   -        -0.6   3,170,707   -    BWI76_RS15125   0.16  +1.3   3,170,814   -    BWI76_RS15125   0.34  +1.2   3,170,822   -    BWI76_RS15125   0.35  +0.0   3,170,880   +    BWI76_RS15125   0.45  +0.9   3,170,991   +    BWI76_RS15125   0.65  +1.2   3,171,183   -        -1.9   3,171,359   +    BWI76_RS15130   0.37  -1.9   3,171,413   -    BWI76_RS15130   0.49  -0.4   3,171,745   -        +1.1   3,171,904   -        -1.0   3,172,060   -    BWI76_RS15135   0.42  +0.7   3,172,090   -    BWI76_RS15135   0.52  -0.2   3,172,159   +    BWI76_RS15135   0.76  +0.8   3,172,167   -    BWI76_RS15135   0.78  +0.4   3,172,346   +        +0.9   3,172,542   -        +1.0 
 
Or see this region's nucleotide sequence