Experiment: Ying_Others16 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4268 and Pf6N2E2_4269 are separated by 106 nucleotides Pf6N2E2_4269 and Pf6N2E2_4270 are separated by 304 nucleotides
Pf6N2E2_4268 - Pyridoxal kinase (EC 2.7.1.35), at 4,965,148 to 4,966,020
_4268
Pf6N2E2_4269 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 4,966,127 to 4,966,585
_4269
Pf6N2E2_4270 - putative Glutathione-regulated potassium-efflux system protein KefB, at 4,966,890 to 4,968,653
_4270
Position (kb)
4966
4967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4965.156 kb on + strand at 4965.265 kb on - strand, within Pf6N2E2_4268 at 4965.292 kb on + strand, within Pf6N2E2_4268 at 4965.344 kb on + strand, within Pf6N2E2_4268 at 4965.344 kb on + strand, within Pf6N2E2_4268 at 4965.344 kb on + strand, within Pf6N2E2_4268 at 4965.344 kb on + strand, within Pf6N2E2_4268 at 4965.344 kb on + strand, within Pf6N2E2_4268 at 4965.345 kb on - strand, within Pf6N2E2_4268 at 4965.345 kb on - strand, within Pf6N2E2_4268 at 4965.345 kb on - strand, within Pf6N2E2_4268 at 4965.345 kb on - strand, within Pf6N2E2_4268 at 4965.345 kb on - strand, within Pf6N2E2_4268 at 4965.478 kb on + strand, within Pf6N2E2_4268 at 4965.478 kb on + strand, within Pf6N2E2_4268 at 4965.479 kb on - strand, within Pf6N2E2_4268 at 4965.479 kb on - strand, within Pf6N2E2_4268 at 4965.479 kb on - strand at 4965.851 kb on + strand, within Pf6N2E2_4268 at 4965.851 kb on + strand, within Pf6N2E2_4268 at 4965.854 kb on + strand, within Pf6N2E2_4268 at 4965.854 kb on + strand, within Pf6N2E2_4268 at 4965.854 kb on + strand, within Pf6N2E2_4268 at 4965.854 kb on + strand, within Pf6N2E2_4268 at 4965.855 kb on - strand, within Pf6N2E2_4268 at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.940 kb on + strand at 4965.941 kb on - strand at 4966.078 kb on + strand at 4966.079 kb on - strand at 4966.079 kb on - strand at 4966.079 kb on - strand at 4966.079 kb on - strand at 4966.109 kb on + strand at 4966.110 kb on - strand at 4966.110 kb on - strand at 4966.110 kb on - strand at 4966.110 kb on - strand at 4966.110 kb on - strand at 4966.261 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.380 kb on + strand, within Pf6N2E2_4269 at 4966.381 kb on - strand, within Pf6N2E2_4269 at 4966.381 kb on - strand, within Pf6N2E2_4269 at 4966.467 kb on + strand, within Pf6N2E2_4269 at 4966.467 kb on + strand, within Pf6N2E2_4269 at 4966.479 kb on + strand, within Pf6N2E2_4269 at 4966.497 kb on + strand, within Pf6N2E2_4269 at 4966.498 kb on - strand, within Pf6N2E2_4269 at 4966.498 kb on - strand, within Pf6N2E2_4269 at 4966.498 kb on - strand, within Pf6N2E2_4269 at 4966.498 kb on - strand, within Pf6N2E2_4269 at 4966.515 kb on + strand, within Pf6N2E2_4269 at 4966.516 kb on - strand, within Pf6N2E2_4269 at 4966.516 kb on - strand, within Pf6N2E2_4269 at 4966.617 kb on + strand at 4966.617 kb on + strand at 4966.617 kb on + strand at 4966.617 kb on + strand at 4966.617 kb on + strand at 4966.618 kb on - strand at 4966.618 kb on - strand at 4966.618 kb on - strand at 4966.618 kb on - strand at 4966.697 kb on - strand at 4966.762 kb on + strand at 4966.762 kb on + strand at 4966.762 kb on + strand at 4966.763 kb on - strand at 4966.763 kb on - strand at 4966.763 kb on - strand at 4966.763 kb on - strand at 4966.763 kb on - strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.764 kb on + strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.765 kb on - strand at 4966.984 kb on + strand at 4966.985 kb on - strand at 4966.992 kb on + strand at 4967.024 kb on + strand at 4967.024 kb on + strand at 4967.042 kb on + strand at 4967.043 kb on - strand at 4967.202 kb on + strand, within Pf6N2E2_4270 at 4967.520 kb on + strand, within Pf6N2E2_4270 at 4967.521 kb on - strand, within Pf6N2E2_4270 at 4967.521 kb on - strand, within Pf6N2E2_4270
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep C; time point 2 remove 4,965,156 + +1.2 4,965,265 - Pf6N2E2_4268 0.13 +0.9 4,965,292 + Pf6N2E2_4268 0.16 -0.5 4,965,344 + Pf6N2E2_4268 0.22 -1.8 4,965,344 + Pf6N2E2_4268 0.22 -0.4 4,965,344 + Pf6N2E2_4268 0.22 -0.8 4,965,344 + Pf6N2E2_4268 0.22 +0.2 4,965,344 + Pf6N2E2_4268 0.22 -0.4 4,965,345 - Pf6N2E2_4268 0.23 -0.4 4,965,345 - Pf6N2E2_4268 0.23 -0.4 4,965,345 - Pf6N2E2_4268 0.23 +1.2 4,965,345 - Pf6N2E2_4268 0.23 -3.0 4,965,345 - Pf6N2E2_4268 0.23 -0.6 4,965,478 + Pf6N2E2_4268 0.38 -0.7 4,965,478 + Pf6N2E2_4268 0.38 +0.2 4,965,479 - Pf6N2E2_4268 0.38 -0.8 4,965,479 - Pf6N2E2_4268 0.38 +0.2 4,965,479 - +1.2 4,965,851 + Pf6N2E2_4268 0.81 -1.2 4,965,851 + Pf6N2E2_4268 0.81 +0.2 4,965,854 + Pf6N2E2_4268 0.81 -2.0 4,965,854 + Pf6N2E2_4268 0.81 -0.4 4,965,854 + Pf6N2E2_4268 0.81 -0.5 4,965,854 + Pf6N2E2_4268 0.81 +1.2 4,965,855 - Pf6N2E2_4268 0.81 +1.2 4,965,940 + +1.2 4,965,940 + +1.2 4,965,940 + +1.2 4,965,940 + -0.4 4,965,940 + +1.2 4,965,940 + +0.6 4,965,940 + +1.2 4,965,940 + -0.8 4,965,940 + -0.4 4,965,941 - +0.2 4,966,078 + +1.2 4,966,079 - -1.8 4,966,079 - -0.3 4,966,079 - +0.2 4,966,079 - +1.2 4,966,109 + +0.2 4,966,110 - +0.2 4,966,110 - +1.2 4,966,110 - +1.2 4,966,110 - +0.2 4,966,110 - +0.2 4,966,261 + Pf6N2E2_4269 0.29 +0.2 4,966,380 + Pf6N2E2_4269 0.55 -1.4 4,966,380 + Pf6N2E2_4269 0.55 +0.0 4,966,380 + Pf6N2E2_4269 0.55 -0.4 4,966,380 + Pf6N2E2_4269 0.55 +0.2 4,966,380 + Pf6N2E2_4269 0.55 -0.4 4,966,380 + Pf6N2E2_4269 0.55 +0.2 4,966,380 + Pf6N2E2_4269 0.55 +0.2 4,966,381 - Pf6N2E2_4269 0.55 +0.2 4,966,381 - Pf6N2E2_4269 0.55 -0.4 4,966,467 + Pf6N2E2_4269 0.74 +0.2 4,966,467 + Pf6N2E2_4269 0.74 +1.2 4,966,479 + Pf6N2E2_4269 0.77 -0.8 4,966,497 + Pf6N2E2_4269 0.81 +0.2 4,966,498 - Pf6N2E2_4269 0.81 +1.2 4,966,498 - Pf6N2E2_4269 0.81 -0.0 4,966,498 - Pf6N2E2_4269 0.81 +1.2 4,966,498 - Pf6N2E2_4269 0.81 -0.4 4,966,515 + Pf6N2E2_4269 0.85 -1.6 4,966,516 - Pf6N2E2_4269 0.85 +1.2 4,966,516 - Pf6N2E2_4269 0.85 +0.2 4,966,617 + +1.2 4,966,617 + +1.2 4,966,617 + -0.8 4,966,617 + +0.2 4,966,617 + +1.2 4,966,618 - +1.2 4,966,618 - +1.2 4,966,618 - -0.8 4,966,618 - +1.2 4,966,697 - +1.2 4,966,762 + +0.2 4,966,762 + -0.3 4,966,762 + -1.4 4,966,763 - +0.2 4,966,763 - -0.4 4,966,763 - +0.2 4,966,763 - -0.4 4,966,763 - -0.4 4,966,764 + -0.4 4,966,764 + -0.6 4,966,764 + +0.2 4,966,764 + -0.4 4,966,764 + +0.7 4,966,764 + -0.7 4,966,764 + -0.4 4,966,764 + +2.2 4,966,764 + +0.2 4,966,764 + -0.4 4,966,764 + +1.2 4,966,765 - -1.8 4,966,765 - +0.9 4,966,765 - +0.2 4,966,765 - +1.2 4,966,765 - -0.2 4,966,765 - -1.3 4,966,765 - +0.2 4,966,765 - -0.4 4,966,765 - +0.2 4,966,765 - +1.2 4,966,765 - -1.2 4,966,984 + +0.2 4,966,985 - -0.4 4,966,992 + +0.2 4,967,024 + +0.2 4,967,024 + -1.2 4,967,042 + +1.2 4,967,043 - +0.2 4,967,202 + Pf6N2E2_4270 0.18 -0.4 4,967,520 + Pf6N2E2_4270 0.36 +1.2 4,967,521 - Pf6N2E2_4270 0.36 +1.2 4,967,521 - Pf6N2E2_4270 0.36 +0.2
Or see this region's nucleotide sequence