Experiment: Ying_Others16 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1143 and Pf6N2E2_1144 are separated by 16 nucleotides Pf6N2E2_1144 and Pf6N2E2_1145 are separated by 13 nucleotides Pf6N2E2_1145 and Pf6N2E2_1146 are separated by 9 nucleotides
Pf6N2E2_1143 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 1,339,620 to 1,341,293
_1143
Pf6N2E2_1144 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 1,341,310 to 1,342,071
_1144
Pf6N2E2_1145 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 1,342,085 to 1,343,275
_1145
Pf6N2E2_1146 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,343,285 to 1,344,412
_1146
Position (kb)
1342
1343
1344 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1341.230 kb on + strand at 1341.231 kb on - strand at 1341.999 kb on + strand at 1341.999 kb on + strand at 1341.999 kb on + strand at 1342.000 kb on - strand at 1342.000 kb on - strand at 1342.057 kb on - strand at 1342.057 kb on - strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.069 kb on + strand at 1342.070 kb on - strand at 1342.070 kb on - strand at 1342.070 kb on - strand at 1342.073 kb on + strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.074 kb on - strand at 1342.102 kb on + strand at 1342.102 kb on + strand at 1342.102 kb on + strand at 1342.103 kb on - strand at 1343.270 kb on + strand at 1343.270 kb on + strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.273 kb on + strand at 1343.281 kb on + strand at 1343.281 kb on + strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.973 kb on - strand, within Pf6N2E2_1146 at 1344.068 kb on + strand, within Pf6N2E2_1146 at 1344.069 kb on - strand, within Pf6N2E2_1146 at 1344.179 kb on + strand, within Pf6N2E2_1146
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep C; time point 2 remove 1,341,230 + -0.2 1,341,231 - -0.2 1,341,999 + -0.9 1,341,999 + +0.8 1,341,999 + -0.3 1,342,000 - -0.8 1,342,000 - -1.8 1,342,057 - +0.0 1,342,057 - +0.8 1,342,069 + +0.8 1,342,069 + -1.0 1,342,069 + +0.8 1,342,069 + -1.2 1,342,069 + -0.2 1,342,069 + +0.8 1,342,069 + -3.4 1,342,070 - -1.8 1,342,070 - +0.2 1,342,070 - +0.0 1,342,073 + -0.8 1,342,074 - -0.2 1,342,074 - -0.2 1,342,074 - -1.7 1,342,074 - -0.5 1,342,102 + -1.7 1,342,102 + +0.8 1,342,102 + -0.7 1,342,103 - -0.2 1,343,270 + +0.8 1,343,270 + -0.2 1,343,271 - -2.0 1,343,271 - -1.5 1,343,271 - +0.8 1,343,273 + +0.8 1,343,281 + -0.2 1,343,281 + -0.2 1,343,282 - -0.2 1,343,282 - +0.8 1,343,282 - +0.8 1,343,483 + Pf6N2E2_1146 0.18 +0.8 1,343,483 + Pf6N2E2_1146 0.18 -0.8 1,343,720 + Pf6N2E2_1146 0.39 -0.5 1,343,720 + Pf6N2E2_1146 0.39 +0.8 1,343,720 + Pf6N2E2_1146 0.39 +0.8 1,343,720 + Pf6N2E2_1146 0.39 -0.2 1,343,720 + Pf6N2E2_1146 0.39 +0.8 1,343,721 - Pf6N2E2_1146 0.39 -2.0 1,343,721 - Pf6N2E2_1146 0.39 +0.8 1,343,972 + Pf6N2E2_1146 0.61 -0.2 1,343,972 + Pf6N2E2_1146 0.61 +0.8 1,343,973 - Pf6N2E2_1146 0.61 +0.8 1,344,068 + Pf6N2E2_1146 0.69 +0.8 1,344,069 - Pf6N2E2_1146 0.70 +0.4 1,344,179 + Pf6N2E2_1146 0.79 +0.8
Or see this region's nucleotide sequence