Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_695

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_694 and Pf6N2E2_695 are separated by 99 nucleotidesPf6N2E2_695 and Pf6N2E2_696 overlap by 4 nucleotidesPf6N2E2_696 and Pf6N2E2_697 are separated by 40 nucleotides Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415 _694 Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011 _695 Pf6N2E2_696 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 803,008 to 803,850 _696 Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069 _697 Position (kb) 801 802 803 804Strain fitness (log2 ratio) -2 -1 0 1at 800.814 kb on - strand, within Pf6N2E2_694at 800.843 kb on + strand, within Pf6N2E2_694at 800.843 kb on + strand, within Pf6N2E2_694at 800.844 kb on - strand, within Pf6N2E2_694at 800.844 kb on - strand, within Pf6N2E2_694at 800.844 kb on - strand, within Pf6N2E2_694at 800.864 kb on + strand, within Pf6N2E2_694at 800.925 kb on - strand, within Pf6N2E2_694at 801.051 kb on - strand, within Pf6N2E2_694at 801.068 kb on + strand, within Pf6N2E2_694at 801.068 kb on + strand, within Pf6N2E2_694at 801.068 kb on + strand, within Pf6N2E2_694at 801.485 kb on + strandat 801.561 kb on + strandat 801.561 kb on + strandat 801.562 kb on - strandat 801.562 kb on - strandat 801.562 kb on - strandat 801.807 kb on + strand, within Pf6N2E2_695at 801.957 kb on + strand, within Pf6N2E2_695at 801.958 kb on - strand, within Pf6N2E2_695at 802.124 kb on - strand, within Pf6N2E2_695at 802.224 kb on + strand, within Pf6N2E2_695at 802.432 kb on - strand, within Pf6N2E2_695at 802.726 kb on - strand, within Pf6N2E2_695at 802.917 kb on + strandat 802.917 kb on + strandat 802.917 kb on + strandat 802.917 kb on + strandat 802.918 kb on - strandat 802.918 kb on - strandat 802.982 kb on + strandat 802.982 kb on + strandat 802.983 kb on - strandat 802.985 kb on + strandat 802.985 kb on + strandat 802.985 kb on + strandat 802.986 kb on - strandat 802.986 kb on - strandat 802.986 kb on - strandat 803.232 kb on + strand, within Pf6N2E2_696at 803.232 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.297 kb on + strand, within Pf6N2E2_696at 803.429 kb on + strand, within Pf6N2E2_696at 803.429 kb on + strand, within Pf6N2E2_696at 803.430 kb on - strand, within Pf6N2E2_696at 803.430 kb on - strand, within Pf6N2E2_696at 803.499 kb on - strand, within Pf6N2E2_696at 803.646 kb on - strand, within Pf6N2E2_696at 803.705 kb on + strand, within Pf6N2E2_696at 803.706 kb on - strand, within Pf6N2E2_696at 803.706 kb on - strand, within Pf6N2E2_696at 803.706 kb on - strand, within Pf6N2E2_696

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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800,814 - Pf6N2E2_694 0.54 -0.8
800,843 + Pf6N2E2_694 0.56 +0.8
800,843 + Pf6N2E2_694 0.56 -0.8
800,844 - Pf6N2E2_694 0.56 +0.8
800,844 - Pf6N2E2_694 0.56 +0.8
800,844 - Pf6N2E2_694 0.56 +0.8
800,864 + Pf6N2E2_694 0.57 -2.6
800,925 - Pf6N2E2_694 0.62 +0.8
801,051 - Pf6N2E2_694 0.72 -0.5
801,068 + Pf6N2E2_694 0.73 -0.2
801,068 + Pf6N2E2_694 0.73 +0.8
801,068 + Pf6N2E2_694 0.73 -0.9
801,485 + +0.2
801,561 + +0.8
801,561 + +0.8
801,562 - +0.8
801,562 - +0.8
801,562 - -0.2
801,807 + Pf6N2E2_695 0.20 +0.8
801,957 + Pf6N2E2_695 0.30 -0.2
801,958 - Pf6N2E2_695 0.30 -0.8
802,124 - Pf6N2E2_695 0.41 +0.8
802,224 + Pf6N2E2_695 0.47 +0.8
802,432 - Pf6N2E2_695 0.61 +0.8
802,726 - Pf6N2E2_695 0.81 -0.8
802,917 + -0.2
802,917 + +0.3
802,917 + +0.8
802,917 + +0.8
802,918 - +0.8
802,918 - +0.8
802,982 + -0.2
802,982 + +0.8
802,983 - +0.8
802,985 + +0.8
802,985 + -0.2
802,985 + +0.8
802,986 - +0.8
802,986 - +0.8
802,986 - +0.8
803,232 + Pf6N2E2_696 0.27 +0.4
803,232 + Pf6N2E2_696 0.27 +0.8
803,297 + Pf6N2E2_696 0.34 -1.0
803,297 + Pf6N2E2_696 0.34 +0.8
803,297 + Pf6N2E2_696 0.34 +0.8
803,297 + Pf6N2E2_696 0.34 -0.2
803,429 + Pf6N2E2_696 0.50 -0.2
803,429 + Pf6N2E2_696 0.50 +0.8
803,430 - Pf6N2E2_696 0.50 +0.8
803,430 - Pf6N2E2_696 0.50 +0.8
803,499 - Pf6N2E2_696 0.58 -0.9
803,646 - Pf6N2E2_696 0.76 +0.8
803,705 + Pf6N2E2_696 0.83 -0.1
803,706 - Pf6N2E2_696 0.83 -0.2
803,706 - Pf6N2E2_696 0.83 -1.0
803,706 - Pf6N2E2_696 0.83 +0.8

Or see this region's nucleotide sequence