Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5221

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5220 and Pf6N2E2_5221 overlap by 4 nucleotidesPf6N2E2_5221 and Pf6N2E2_5222 are separated by 14 nucleotidesPf6N2E2_5222 and Pf6N2E2_5223 overlap by 7 nucleotides Pf6N2E2_5220 - Glutamyl-tRNA reductase (EC 1.2.1.70), at 6,018,693 to 6,019,922 _5220 Pf6N2E2_5221 - Peptide chain release factor 1, at 6,019,919 to 6,021,001 _5221 Pf6N2E2_5222 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 6,021,016 to 6,021,846 _5222 Pf6N2E2_5223 - Molybdopterin biosynthesis protein MoeB, at 6,021,840 to 6,022,595 _5223 Position (kb) 6019 6020 6021 6022Strain fitness (log2 ratio) -2 -1 0 1at 6020.999 kb on + strandat 6021.013 kb on + strandat 6021.013 kb on + strandat 6021.013 kb on + strandat 6021.978 kb on + strand, within Pf6N2E2_5223at 6021.978 kb on + strand, within Pf6N2E2_5223at 6021.978 kb on + strand, within Pf6N2E2_5223at 6021.978 kb on + strand, within Pf6N2E2_5223

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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6,020,999 + +0.1
6,021,013 + +1.1
6,021,013 + +1.1
6,021,013 + -2.4
6,021,978 + Pf6N2E2_5223 0.18 +0.1
6,021,978 + Pf6N2E2_5223 0.18 -1.7
6,021,978 + Pf6N2E2_5223 0.18 -0.5
6,021,978 + Pf6N2E2_5223 0.18 +0.1

Or see this region's nucleotide sequence