Experiment: Ying_Others16 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1100 and Pf6N2E2_1101 are separated by 54 nucleotides Pf6N2E2_1101 and Pf6N2E2_1102 are separated by 186 nucleotides Pf6N2E2_1102 and Pf6N2E2_1103 are separated by 11 nucleotides Pf6N2E2_1103 and Pf6N2E2_1104 overlap by 4 nucleotides
Pf6N2E2_1100 - L-rhamnose operon transcriptional activator RhaR, at 1,286,629 to 1,287,465
_1100
Pf6N2E2_1101 - Transporter, LysE family, at 1,287,520 to 1,288,122
_1101
Pf6N2E2_1102 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 1,288,309 to 1,289,742
_1102
Pf6N2E2_1103 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,289,754 to 1,290,554
_1103
Pf6N2E2_1104 - Gluconate dehydratase (EC 4.2.1.39), at 1,290,551 to 1,291,750
_1104
Position (kb)
1288
1289
1290 Strain fitness (log2 ratio)
-2
-1
0
1 at 1287.434 kb on - strand at 1287.531 kb on - strand at 1287.603 kb on + strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.752 kb on + strand, within Pf6N2E2_1101 at 1288.009 kb on + strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.300 kb on + strand at 1288.322 kb on + strand at 1288.336 kb on - strand at 1288.344 kb on - strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.350 kb on - strand at 1288.350 kb on - strand at 1288.393 kb on + strand at 1288.442 kb on + strand at 1288.442 kb on + strand at 1288.442 kb on + strand at 1288.443 kb on - strand at 1288.443 kb on - strand at 1288.443 kb on - strand at 1288.524 kb on - strand, within Pf6N2E2_1102 at 1288.629 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.670 kb on - strand at 1289.758 kb on + strand at 1289.759 kb on - strand at 1289.973 kb on + strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1290.145 kb on + strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.359 kb on - strand, within Pf6N2E2_1103
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2 remove 1,287,434 - -2.0 1,287,531 - -0.2 1,287,603 + Pf6N2E2_1101 0.14 -0.2 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,604 - Pf6N2E2_1101 0.14 -1.0 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,604 - Pf6N2E2_1101 0.14 -0.2 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,752 + Pf6N2E2_1101 0.38 -0.8 1,288,009 + Pf6N2E2_1101 0.81 -0.2 1,288,010 - Pf6N2E2_1101 0.81 -1.1 1,288,010 - Pf6N2E2_1101 0.81 -0.5 1,288,010 - Pf6N2E2_1101 0.81 -0.2 1,288,300 + -0.2 1,288,322 + -0.8 1,288,336 - +0.8 1,288,344 - +0.8 1,288,349 + +0.8 1,288,349 + +0.8 1,288,349 + -0.8 1,288,349 + +0.8 1,288,350 - +0.8 1,288,350 - -0.2 1,288,393 + +0.8 1,288,442 + -0.2 1,288,442 + +0.8 1,288,442 + +0.8 1,288,443 - +0.8 1,288,443 - -0.2 1,288,443 - -1.8 1,288,524 - Pf6N2E2_1102 0.15 +0.8 1,288,629 + Pf6N2E2_1102 0.22 +0.8 1,288,877 + Pf6N2E2_1102 0.40 -0.2 1,288,877 + Pf6N2E2_1102 0.40 -1.5 1,288,877 + Pf6N2E2_1102 0.40 -1.2 1,289,408 + Pf6N2E2_1102 0.77 -0.6 1,289,408 + Pf6N2E2_1102 0.77 +0.8 1,289,408 + Pf6N2E2_1102 0.77 -1.0 1,289,409 - Pf6N2E2_1102 0.77 +0.8 1,289,409 - Pf6N2E2_1102 0.77 -0.8 1,289,417 + Pf6N2E2_1102 0.77 -0.2 1,289,417 + Pf6N2E2_1102 0.77 -0.2 1,289,417 + Pf6N2E2_1102 0.77 +0.8 1,289,417 + Pf6N2E2_1102 0.77 +0.8 1,289,417 + Pf6N2E2_1102 0.77 +0.8 1,289,418 - Pf6N2E2_1102 0.77 +0.8 1,289,418 - Pf6N2E2_1102 0.77 -0.2 1,289,669 + +0.8 1,289,669 + -1.9 1,289,669 + -0.8 1,289,669 + +0.8 1,289,669 + -0.6 1,289,670 - -0.4 1,289,758 + +0.8 1,289,759 - -0.2 1,289,973 + Pf6N2E2_1103 0.27 -0.5 1,289,974 - Pf6N2E2_1103 0.27 -0.2 1,289,974 - Pf6N2E2_1103 0.27 -1.5 1,290,145 + Pf6N2E2_1103 0.49 +0.1 1,290,146 - Pf6N2E2_1103 0.49 -0.2 1,290,146 - Pf6N2E2_1103 0.49 -0.0 1,290,146 - Pf6N2E2_1103 0.49 +0.8 1,290,359 - Pf6N2E2_1103 0.76 +0.8
Or see this region's nucleotide sequence