Experiment: Ying_Others16 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1073 and Pf6N2E2_1074 are separated by 229 nucleotides Pf6N2E2_1074 and Pf6N2E2_1075 are separated by 20 nucleotides Pf6N2E2_1075 and Pf6N2E2_1076 overlap by 4 nucleotides
Pf6N2E2_1073 - Glycogen debranching enzyme (EC 3.2.1.-), at 1,251,877 to 1,254,036
_1073
Pf6N2E2_1074 - FIG00953356: hypothetical protein, at 1,254,266 to 1,254,619
_1074
Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426
_1075
Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501
_1076
Position (kb)
1254
1255
1256
1257
1258 Strain fitness (log2 ratio)
-2
-1
0
1 at 1253.765 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.780 kb on + strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.781 kb on - strand, within Pf6N2E2_1073 at 1253.819 kb on + strand, within Pf6N2E2_1073 at 1253.834 kb on + strand at 1253.834 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.036 kb on + strand at 1254.037 kb on - strand at 1254.037 kb on - strand at 1254.132 kb on + strand at 1254.133 kb on - strand at 1254.133 kb on - strand at 1254.560 kb on - strand, within Pf6N2E2_1074 at 1254.583 kb on - strand, within Pf6N2E2_1074 at 1254.850 kb on + strand at 1254.850 kb on + strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.955 kb on + strand, within Pf6N2E2_1075 at 1254.956 kb on - strand, within Pf6N2E2_1075 at 1255.149 kb on - strand, within Pf6N2E2_1075 at 1255.149 kb on - strand, within Pf6N2E2_1075 at 1255.316 kb on + strand, within Pf6N2E2_1075 at 1255.556 kb on + strand, within Pf6N2E2_1075 at 1255.557 kb on - strand, within Pf6N2E2_1075 at 1255.721 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.006 kb on + strand, within Pf6N2E2_1075 at 1256.007 kb on - strand, within Pf6N2E2_1075 at 1256.016 kb on - strand, within Pf6N2E2_1075 at 1256.522 kb on - strand, within Pf6N2E2_1075 at 1256.681 kb on + strand, within Pf6N2E2_1075 at 1256.787 kb on - strand, within Pf6N2E2_1075 at 1256.921 kb on + strand, within Pf6N2E2_1075 at 1256.922 kb on - strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.169 kb on - strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.303 kb on - strand at 1257.465 kb on + strand at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.073 kb on - strand, within Pf6N2E2_1076
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2 remove 1,253,765 + Pf6N2E2_1073 0.87 -1.5 1,253,780 + Pf6N2E2_1073 0.88 -1.7 1,253,780 + Pf6N2E2_1073 0.88 -1.4 1,253,780 + Pf6N2E2_1073 0.88 -1.7 1,253,780 + Pf6N2E2_1073 0.88 +0.8 1,253,780 + Pf6N2E2_1073 0.88 -0.2 1,253,780 + Pf6N2E2_1073 0.88 -2.0 1,253,780 + Pf6N2E2_1073 0.88 -0.2 1,253,780 + Pf6N2E2_1073 0.88 +0.8 1,253,780 + Pf6N2E2_1073 0.88 +0.8 1,253,781 - Pf6N2E2_1073 0.88 +0.8 1,253,781 - Pf6N2E2_1073 0.88 +0.8 1,253,781 - Pf6N2E2_1073 0.88 +0.8 1,253,819 + Pf6N2E2_1073 0.90 +0.8 1,253,834 + +0.8 1,253,834 + +0.8 1,254,036 + -1.5 1,254,036 + +0.8 1,254,036 + -0.8 1,254,036 + -0.3 1,254,036 + -2.1 1,254,037 - -1.2 1,254,037 - +0.8 1,254,132 + +0.8 1,254,133 - +0.8 1,254,133 - -0.7 1,254,560 - Pf6N2E2_1074 0.83 +1.2 1,254,583 - Pf6N2E2_1074 0.90 +0.8 1,254,850 + -2.2 1,254,850 + -0.2 1,254,851 - -0.8 1,254,851 - +0.8 1,254,851 - +0.8 1,254,955 + Pf6N2E2_1075 0.11 -0.5 1,254,955 + Pf6N2E2_1075 0.11 -0.2 1,254,956 - Pf6N2E2_1075 0.11 -0.4 1,255,149 - Pf6N2E2_1075 0.18 +0.8 1,255,149 - Pf6N2E2_1075 0.18 -0.7 1,255,316 + Pf6N2E2_1075 0.24 +0.8 1,255,556 + Pf6N2E2_1075 0.33 -1.0 1,255,557 - Pf6N2E2_1075 0.33 +0.8 1,255,721 + Pf6N2E2_1075 0.39 -1.6 1,256,006 + Pf6N2E2_1075 0.49 +0.8 1,256,006 + Pf6N2E2_1075 0.49 -0.6 1,256,006 + Pf6N2E2_1075 0.49 -1.1 1,256,006 + Pf6N2E2_1075 0.49 -1.2 1,256,006 + Pf6N2E2_1075 0.49 -0.2 1,256,007 - Pf6N2E2_1075 0.49 +0.8 1,256,016 - Pf6N2E2_1075 0.49 +0.8 1,256,522 - Pf6N2E2_1075 0.68 +1.0 1,256,681 + Pf6N2E2_1075 0.73 -1.5 1,256,787 - Pf6N2E2_1075 0.77 +0.8 1,256,921 + Pf6N2E2_1075 0.82 +0.8 1,256,922 - Pf6N2E2_1075 0.82 +0.8 1,257,068 + Pf6N2E2_1075 0.87 -0.8 1,257,068 + Pf6N2E2_1075 0.87 +0.8 1,257,169 - +0.8 1,257,302 + +0.8 1,257,302 + -0.6 1,257,302 + -0.8 1,257,302 + +0.8 1,257,303 - +0.8 1,257,465 + +0.8 1,258,072 + Pf6N2E2_1076 0.31 -1.4 1,258,072 + Pf6N2E2_1076 0.31 +0.8 1,258,072 + Pf6N2E2_1076 0.31 +0.8 1,258,073 - Pf6N2E2_1076 0.31 +0.8
Or see this region's nucleotide sequence