Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_106

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_104 and Pf6N2E2_105 overlap by 4 nucleotidesPf6N2E2_105 and Pf6N2E2_106 are separated by 10 nucleotidesPf6N2E2_106 and Pf6N2E2_107 are separated by 10 nucleotidesPf6N2E2_107 and Pf6N2E2_108 are separated by 48 nucleotides Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747 _104 Pf6N2E2_105 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 150,744 to 151,610 _105 Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421 _106 Pf6N2E2_107 - Probable transmembrane protein, at 152,432 to 152,704 _107 Pf6N2E2_108 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 152,753 to 154,495 _108 Position (kb) 151 152 153Strain fitness (log2 ratio) -3 -2 -1 0 1at 150.627 kb on - strand, within Pf6N2E2_104at 150.667 kb on + strandat 150.667 kb on + strandat 150.667 kb on + strandat 150.701 kb on - strandat 150.712 kb on + strandat 150.712 kb on + strandat 150.879 kb on + strand, within Pf6N2E2_105at 151.167 kb on + strand, within Pf6N2E2_105at 151.167 kb on + strand, within Pf6N2E2_105at 151.168 kb on - strand, within Pf6N2E2_105at 151.168 kb on - strand, within Pf6N2E2_105at 151.173 kb on + strand, within Pf6N2E2_105at 151.173 kb on + strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.258 kb on - strand, within Pf6N2E2_105at 151.287 kb on + strand, within Pf6N2E2_105at 151.288 kb on - strand, within Pf6N2E2_105at 151.288 kb on - strand, within Pf6N2E2_105at 151.290 kb on + strand, within Pf6N2E2_105at 151.290 kb on + strandat 151.290 kb on + strand, within Pf6N2E2_105at 151.291 kb on - strand, within Pf6N2E2_105at 151.413 kb on + strand, within Pf6N2E2_105at 151.414 kb on - strand, within Pf6N2E2_105at 151.540 kb on - strandat 151.540 kb on - strandat 151.540 kb on - strandat 151.540 kb on - strandat 151.575 kb on + strandat 151.575 kb on + strandat 151.654 kb on + strandat 151.655 kb on - strandat 151.655 kb on - strandat 151.655 kb on - strandat 151.681 kb on + strandat 151.695 kb on + strandat 151.695 kb on + strandat 151.696 kb on - strandat 151.768 kb on + strand, within Pf6N2E2_106at 151.768 kb on + strand, within Pf6N2E2_106at 151.768 kb on + strand, within Pf6N2E2_106at 151.769 kb on - strand, within Pf6N2E2_106at 151.798 kb on + strand, within Pf6N2E2_106at 151.823 kb on - strand, within Pf6N2E2_106at 151.825 kb on + strand, within Pf6N2E2_106at 151.826 kb on - strand, within Pf6N2E2_106at 151.826 kb on - strand, within Pf6N2E2_106at 151.984 kb on + strand, within Pf6N2E2_106at 151.984 kb on + strand, within Pf6N2E2_106at 151.985 kb on - strand, within Pf6N2E2_106at 151.985 kb on - strand, within Pf6N2E2_106at 152.117 kb on - strand, within Pf6N2E2_106at 152.703 kb on - strandat 152.731 kb on + strandat 152.752 kb on + strandat 152.753 kb on - strandat 152.753 kb on - strandat 152.753 kb on - strandat 152.753 kb on - strandat 152.782 kb on + strandat 152.783 kb on - strandat 152.783 kb on - strandat 152.893 kb on + strandat 152.893 kb on + strandat 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.127 kb on + strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.128 kb on - strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.131 kb on + strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.132 kb on - strand, within Pf6N2E2_108at 153.268 kb on + strand, within Pf6N2E2_108at 153.278 kb on - strand, within Pf6N2E2_108at 153.336 kb on - strand, within Pf6N2E2_108at 153.395 kb on + strand, within Pf6N2E2_108at 153.395 kb on + strand, within Pf6N2E2_108at 153.396 kb on - strand, within Pf6N2E2_108at 153.396 kb on - strand, within Pf6N2E2_108at 153.396 kb on - strand, within Pf6N2E2_108

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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150,627 - Pf6N2E2_104 0.89 +0.9
150,667 + -2.8
150,667 + -1.1
150,667 + -0.1
150,701 - -0.3
150,712 + +0.9
150,712 + +0.9
150,879 + Pf6N2E2_105 0.16 -2.1
151,167 + Pf6N2E2_105 0.49 -0.1
151,167 + Pf6N2E2_105 0.49 +0.9
151,168 - Pf6N2E2_105 0.49 +0.9
151,168 - Pf6N2E2_105 0.49 -0.7
151,173 + Pf6N2E2_105 0.49 -0.7
151,173 + Pf6N2E2_105 0.49 +0.9
151,174 - Pf6N2E2_105 0.50 -0.1
151,174 - Pf6N2E2_105 0.50 +0.9
151,174 - Pf6N2E2_105 0.50 +0.9
151,258 - Pf6N2E2_105 0.59 +0.9
151,287 + Pf6N2E2_105 0.63 +0.9
151,288 - Pf6N2E2_105 0.63 -1.2
151,288 - Pf6N2E2_105 0.63 +0.9
151,290 + Pf6N2E2_105 0.63 +0.9
151,290 + +0.7
151,290 + Pf6N2E2_105 0.63 +0.9
151,291 - Pf6N2E2_105 0.63 +0.9
151,413 + Pf6N2E2_105 0.77 -1.1
151,414 - Pf6N2E2_105 0.77 +0.9
151,540 - +0.9
151,540 - -2.0
151,540 - -0.1
151,540 - -0.7
151,575 + +0.9
151,575 + +0.9
151,654 + -0.1
151,655 - -0.1
151,655 - -1.4
151,655 - +0.9
151,681 + +0.9
151,695 + -0.7
151,695 + -0.7
151,696 - -0.7
151,768 + Pf6N2E2_106 0.18 -1.3
151,768 + Pf6N2E2_106 0.18 +0.9
151,768 + Pf6N2E2_106 0.18 +0.9
151,769 - Pf6N2E2_106 0.18 -0.7
151,798 + Pf6N2E2_106 0.22 -0.1
151,823 - Pf6N2E2_106 0.25 -1.2
151,825 + Pf6N2E2_106 0.25 -0.1
151,826 - Pf6N2E2_106 0.26 -1.4
151,826 - Pf6N2E2_106 0.26 +0.9
151,984 + Pf6N2E2_106 0.45 -1.5
151,984 + Pf6N2E2_106 0.45 -1.9
151,985 - Pf6N2E2_106 0.45 -2.0
151,985 - Pf6N2E2_106 0.45 -0.1
152,117 - Pf6N2E2_106 0.62 -0.6
152,703 - +0.9
152,731 + -0.3
152,752 + +0.5
152,753 - -1.1
152,753 - -0.7
152,753 - +0.9
152,753 - +0.9
152,782 + -0.1
152,783 - +0.9
152,783 - -1.1
152,893 + +0.9
152,893 + +0.9
153,127 + Pf6N2E2_108 0.21 -0.1
153,127 + Pf6N2E2_108 0.21 +0.9
153,127 + Pf6N2E2_108 0.21 +0.9
153,127 + Pf6N2E2_108 0.21 -1.1
153,127 + Pf6N2E2_108 0.21 -0.7
153,127 + Pf6N2E2_108 0.21 +0.9
153,127 + Pf6N2E2_108 0.21 +0.9
153,128 - Pf6N2E2_108 0.22 +0.9
153,128 - Pf6N2E2_108 0.22 -0.7
153,128 - Pf6N2E2_108 0.22 -0.7
153,128 - Pf6N2E2_108 0.22 +0.9
153,128 - Pf6N2E2_108 0.22 +0.9
153,128 - Pf6N2E2_108 0.22 -0.9
153,128 - Pf6N2E2_108 0.22 -1.3
153,131 + Pf6N2E2_108 0.22 -0.1
153,131 + Pf6N2E2_108 0.22 -1.1
153,131 + Pf6N2E2_108 0.22 +0.9
153,131 + Pf6N2E2_108 0.22 +0.9
153,132 - Pf6N2E2_108 0.22 -1.0
153,132 - Pf6N2E2_108 0.22 -0.1
153,132 - Pf6N2E2_108 0.22 -0.1
153,132 - Pf6N2E2_108 0.22 -0.1
153,132 - Pf6N2E2_108 0.22 -0.1
153,132 - Pf6N2E2_108 0.22 +0.9
153,132 - Pf6N2E2_108 0.22 -0.1
153,132 - Pf6N2E2_108 0.22 -0.7
153,132 - Pf6N2E2_108 0.22 -0.1
153,132 - Pf6N2E2_108 0.22 -0.9
153,268 + Pf6N2E2_108 0.30 -0.8
153,278 - Pf6N2E2_108 0.30 -0.1
153,336 - Pf6N2E2_108 0.33 -0.4
153,395 + Pf6N2E2_108 0.37 -0.8
153,395 + Pf6N2E2_108 0.37 +0.9
153,396 - Pf6N2E2_108 0.37 +0.9
153,396 - Pf6N2E2_108 0.37 +0.9
153,396 - Pf6N2E2_108 0.37 -2.0

Or see this region's nucleotide sequence