Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_667 and Pf6N2E2_668 are separated by 55 nucleotides Pf6N2E2_668 and Pf6N2E2_669 are separated by 70 nucleotides Pf6N2E2_669 and Pf6N2E2_670 are separated by 137 nucleotides
Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170
_667
Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320
_668
Pf6N2E2_669 - Nuclease inhibitor, at 774,391 to 774,945
_669
Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000
_670
Position (kb) 773
774
775 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 772.782 kb on - strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.948 kb on - strand, within Pf6N2E2_667 at 772.989 kb on + strand, within Pf6N2E2_667 at 772.990 kb on - strand, within Pf6N2E2_667 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.389 kb on - strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.590 kb on - strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 774.147 kb on + strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.746 kb on - strand, within Pf6N2E2_669 at 774.782 kb on + strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.858 kb on - strand, within Pf6N2E2_669 at 774.938 kb on + strand at 774.938 kb on + strand at 774.939 kb on - strand at 775.072 kb on + strand at 775.084 kb on + strand at 775.147 kb on + strand at 775.147 kb on + strand at 775.148 kb on - strand at 775.148 kb on - strand at 775.288 kb on + strand, within Pf6N2E2_670 at 775.288 kb on + strand at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 772,782 - Pf6N2E2_667 0.65 -0.5 772,895 + Pf6N2E2_667 0.75 -0.2 772,895 + Pf6N2E2_667 0.75 +1.9 772,896 - Pf6N2E2_667 0.75 -0.7 772,896 - Pf6N2E2_667 0.75 -0.1 772,948 - Pf6N2E2_667 0.80 +0.9 772,989 + Pf6N2E2_667 0.84 -0.4 772,990 - Pf6N2E2_667 0.84 +0.9 773,388 + Pf6N2E2_668 0.15 +0.9 773,388 + Pf6N2E2_668 0.15 +0.9 773,389 - Pf6N2E2_668 0.15 +0.9 773,568 + Pf6N2E2_668 0.31 -0.7 773,568 + Pf6N2E2_668 0.31 +1.3 773,569 - Pf6N2E2_668 0.31 -1.5 773,569 - Pf6N2E2_668 0.31 +0.9 773,572 - Pf6N2E2_668 0.32 +0.9 773,572 - Pf6N2E2_668 0.32 +0.9 773,572 - Pf6N2E2_668 0.32 -2.7 773,572 - Pf6N2E2_668 0.32 -0.1 773,590 - Pf6N2E2_668 0.33 -0.1 773,676 + Pf6N2E2_668 0.41 -0.3 773,676 + Pf6N2E2_668 0.41 +0.9 774,147 + Pf6N2E2_668 0.84 +0.9 774,148 - Pf6N2E2_668 0.84 +0.9 774,148 - Pf6N2E2_668 0.84 +0.9 774,148 - Pf6N2E2_668 0.84 +0.9 774,466 - Pf6N2E2_669 0.14 -0.1 774,466 - Pf6N2E2_669 0.14 +0.9 774,746 - Pf6N2E2_669 0.64 -3.3 774,782 + Pf6N2E2_669 0.70 +0.9 774,783 - Pf6N2E2_669 0.71 +0.9 774,783 - Pf6N2E2_669 0.71 -0.7 774,858 - Pf6N2E2_669 0.84 +0.9 774,938 + +0.9 774,938 + -0.1 774,939 - +0.9 775,072 + -0.4 775,084 + +0.9 775,147 + -0.1 775,147 + +0.9 775,148 - +0.9 775,148 - +0.9 775,288 + Pf6N2E2_670 0.22 +0.9 775,288 + +0.9 775,289 - Pf6N2E2_670 0.22 -0.1 775,289 - Pf6N2E2_670 0.22 +0.9 775,289 - Pf6N2E2_670 0.22 -1.1
Or see this region's nucleotide sequence