Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_637 and Pf6N2E2_638 are separated by 195 nucleotides Pf6N2E2_638 and Pf6N2E2_639 are separated by 180 nucleotides Pf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides
Pf6N2E2_637 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 728,851 to 730,284
_637
Pf6N2E2_638 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 730,480 to 730,920
_638
Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442
_639
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Position (kb) 730
731 Strain fitness (log2 ratio) -2
-1
0
1 at 729.531 kb on - strand, within Pf6N2E2_637 at 729.825 kb on - strand, within Pf6N2E2_637 at 729.825 kb on - strand, within Pf6N2E2_637 at 730.061 kb on + strand, within Pf6N2E2_637 at 730.061 kb on + strand, within Pf6N2E2_637 at 730.061 kb on + strand, within Pf6N2E2_637 at 730.062 kb on - strand, within Pf6N2E2_637 at 730.062 kb on - strand, within Pf6N2E2_637 at 730.101 kb on - strand, within Pf6N2E2_637 at 730.101 kb on - strand, within Pf6N2E2_637 at 730.115 kb on + strand, within Pf6N2E2_637 at 730.116 kb on - strand, within Pf6N2E2_637 at 730.232 kb on + strand at 730.233 kb on - strand at 730.233 kb on - strand at 730.233 kb on - strand at 730.358 kb on + strand at 730.359 kb on - strand at 730.426 kb on + strand at 730.498 kb on + strand at 730.498 kb on + strand at 730.501 kb on + strand at 730.501 kb on + strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.801 kb on + strand, within Pf6N2E2_638 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 731.029 kb on - strand at 731.103 kb on - strand at 731.169 kb on + strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.495 kb on + strand at 731.528 kb on + strand at 731.528 kb on + strand at 731.529 kb on - strand at 731.540 kb on - strand at 731.540 kb on - strand at 731.572 kb on + strand at 731.573 kb on - strand at 731.573 kb on - strand at 731.803 kb on + strand, within Pf6N2E2_640 at 731.803 kb on + strand, within Pf6N2E2_640 at 731.804 kb on - strand, within Pf6N2E2_640 at 731.882 kb on + strand, within Pf6N2E2_640
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 729,531 - Pf6N2E2_637 0.47 -0.1 729,825 - Pf6N2E2_637 0.68 -0.1 729,825 - Pf6N2E2_637 0.68 +0.9 730,061 + Pf6N2E2_637 0.84 +0.9 730,061 + Pf6N2E2_637 0.84 -0.1 730,061 + Pf6N2E2_637 0.84 -0.4 730,062 - Pf6N2E2_637 0.84 -0.1 730,062 - Pf6N2E2_637 0.84 +0.9 730,101 - Pf6N2E2_637 0.87 +0.9 730,101 - Pf6N2E2_637 0.87 -0.1 730,115 + Pf6N2E2_637 0.88 -0.7 730,116 - Pf6N2E2_637 0.88 +0.9 730,232 + +0.9 730,233 - +0.9 730,233 - +0.9 730,233 - -0.5 730,358 + -0.9 730,359 - -0.1 730,426 + -0.1 730,498 + +0.9 730,498 + +1.5 730,501 + +0.8 730,501 + +0.9 730,502 - +0.9 730,502 - +0.9 730,502 - +0.3 730,502 - -0.1 730,502 - +0.9 730,801 + Pf6N2E2_638 0.73 -0.1 730,801 + Pf6N2E2_638 0.73 -0.1 730,802 - Pf6N2E2_638 0.73 -1.9 730,802 - Pf6N2E2_638 0.73 +0.9 731,029 - -0.7 731,103 - -2.4 731,169 + Pf6N2E2_639 0.20 +0.9 731,170 - Pf6N2E2_639 0.20 -0.6 731,170 - Pf6N2E2_639 0.20 +0.9 731,170 - Pf6N2E2_639 0.20 +0.9 731,170 - Pf6N2E2_639 0.20 -0.7 731,495 + +0.9 731,528 + +0.9 731,528 + +0.9 731,529 - +0.9 731,540 - -0.1 731,540 - +0.9 731,572 + -0.1 731,573 - -0.7 731,573 - -0.6 731,803 + Pf6N2E2_640 0.24 -0.1 731,803 + Pf6N2E2_640 0.24 +0.9 731,804 - Pf6N2E2_640 0.25 -0.1 731,882 + Pf6N2E2_640 0.37 +0.9
Or see this region's nucleotide sequence