Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_61 and Pf6N2E2_62 overlap by 31 nucleotides Pf6N2E2_62 and Pf6N2E2_63 are separated by 157 nucleotides Pf6N2E2_63 and Pf6N2E2_64 are separated by 11 nucleotides Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides
Pf6N2E2_61 - Cys regulon transcriptional activator CysB, at 101,960 to 102,238
_61
Pf6N2E2_62 - Cys regulon transcriptional activator CysB, at 102,208 to 102,852
_62
Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428
_63
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Position (kb) 103
104
105 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.078 kb on - strand, within Pf6N2E2_61 at 102.168 kb on - strand, within Pf6N2E2_61 at 102.180 kb on + strand, within Pf6N2E2_61 at 102.250 kb on + strand at 102.393 kb on - strand at 102.455 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.823 kb on + strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.826 kb on - strand at 102.826 kb on - strand at 102.920 kb on + strand at 102.921 kb on - strand at 102.929 kb on - strand at 102.955 kb on + strand at 102.981 kb on + strand at 102.982 kb on - strand at 103.028 kb on + strand at 103.028 kb on + strand at 103.088 kb on + strand at 103.088 kb on + strand at 103.088 kb on + strand at 103.206 kb on + strand, within Pf6N2E2_63 at 103.207 kb on - strand, within Pf6N2E2_63 at 103.288 kb on + strand, within Pf6N2E2_63 at 103.748 kb on + strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.749 kb on - strand, within Pf6N2E2_63 at 103.997 kb on + strand, within Pf6N2E2_63 at 103.998 kb on - strand, within Pf6N2E2_63 at 104.318 kb on - strand at 104.350 kb on + strand at 104.602 kb on + strand, within Pf6N2E2_64 at 104.603 kb on - strand, within Pf6N2E2_64 at 104.660 kb on - strand, within Pf6N2E2_64 at 104.774 kb on - strand, within Pf6N2E2_64 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 102,052 + Pf6N2E2_61 0.33 -0.3 102,052 + Pf6N2E2_61 0.33 -0.6 102,052 + Pf6N2E2_61 0.33 -0.1 102,052 + Pf6N2E2_61 0.33 -0.6 102,052 + Pf6N2E2_61 0.33 +0.9 102,052 + Pf6N2E2_61 0.33 -0.6 102,052 + Pf6N2E2_61 0.33 -0.1 102,052 + Pf6N2E2_61 0.33 +0.9 102,053 - Pf6N2E2_61 0.33 -1.1 102,053 - Pf6N2E2_61 0.33 +0.9 102,053 - Pf6N2E2_61 0.33 +0.9 102,053 - Pf6N2E2_61 0.33 -0.9 102,053 - Pf6N2E2_61 0.33 +0.9 102,054 + Pf6N2E2_61 0.34 +0.9 102,054 + Pf6N2E2_61 0.34 +0.9 102,054 + Pf6N2E2_61 0.34 -0.1 102,055 - Pf6N2E2_61 0.34 -0.5 102,055 - Pf6N2E2_61 0.34 +0.9 102,055 - Pf6N2E2_61 0.34 -1.1 102,055 - Pf6N2E2_61 0.34 -0.1 102,059 + Pf6N2E2_61 0.35 -0.1 102,059 + Pf6N2E2_61 0.35 +0.3 102,060 - Pf6N2E2_61 0.36 +0.9 102,060 - Pf6N2E2_61 0.36 -0.9 102,077 + Pf6N2E2_61 0.42 +0.1 102,077 + Pf6N2E2_61 0.42 -0.6 102,077 + Pf6N2E2_61 0.42 -0.1 102,077 + Pf6N2E2_61 0.42 +0.9 102,078 - Pf6N2E2_61 0.42 +0.9 102,168 - Pf6N2E2_61 0.75 -0.5 102,180 + Pf6N2E2_61 0.79 -0.1 102,250 + +0.4 102,393 - -0.8 102,455 + Pf6N2E2_62 0.38 -0.1 102,550 + Pf6N2E2_62 0.53 -0.9 102,550 + Pf6N2E2_62 0.53 -1.1 102,551 - Pf6N2E2_62 0.53 +0.9 102,551 - Pf6N2E2_62 0.53 -0.4 102,551 - Pf6N2E2_62 0.53 -0.8 102,551 - Pf6N2E2_62 0.53 +0.9 102,823 + +0.9 102,824 - +0.9 102,824 - +0.9 102,824 - -0.4 102,824 - -0.1 102,825 + +0.9 102,825 + +0.9 102,825 + +0.9 102,825 + +0.9 102,826 - -0.4 102,826 - -0.4 102,920 + -0.8 102,921 - +0.9 102,929 - -5.4 102,955 + -0.6 102,981 + +0.9 102,982 - +0.9 103,028 + -0.6 103,028 + +0.9 103,088 + -1.4 103,088 + +0.9 103,088 + -0.6 103,206 + Pf6N2E2_63 0.14 +0.9 103,207 - Pf6N2E2_63 0.14 -5.8 103,288 + Pf6N2E2_63 0.20 -3.1 103,748 + Pf6N2E2_63 0.52 -0.1 103,749 - Pf6N2E2_63 0.52 -1.4 103,749 - Pf6N2E2_63 0.52 -0.1 103,997 + Pf6N2E2_63 0.70 -0.1 103,998 - Pf6N2E2_63 0.70 -0.1 104,318 - -0.1 104,350 + -0.6 104,602 + Pf6N2E2_64 0.25 -0.6 104,603 - Pf6N2E2_64 0.25 -0.1 104,660 - Pf6N2E2_64 0.34 -4.6 104,774 - Pf6N2E2_64 0.52 -0.1 105,092 + -0.8 105,251 + -0.9 105,252 - -0.7 105,252 - -0.3 105,283 + +0.9 105,329 + Pf6N2E2_65 0.14 -1.0 105,330 - Pf6N2E2_65 0.14 -0.1 105,330 - Pf6N2E2_65 0.14 +0.2 105,330 - Pf6N2E2_65 0.14 -0.3 105,330 - Pf6N2E2_65 0.14 +0.9 105,330 - Pf6N2E2_65 0.14 +0.9
Or see this region's nucleotide sequence