Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5837 and Pf6N2E2_5838 are separated by 177 nucleotides Pf6N2E2_5838 and Pf6N2E2_5839 are separated by 169 nucleotides
Pf6N2E2_5837 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 6,654,362 to 6,655,252
_5837
Pf6N2E2_5838 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 6,655,430 to 6,656,212
_5838
Pf6N2E2_5839 - Glyoxylate carboligase (EC 4.1.1.47), at 6,656,382 to 6,658,148
_5839
Position (kb)
6655
6656
6657 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 6654.481 kb on + strand, within Pf6N2E2_5837 at 6655.272 kb on + strand at 6655.272 kb on + strand at 6655.272 kb on + strand at 6655.272 kb on + strand at 6655.273 kb on - strand at 6655.273 kb on - strand at 6655.491 kb on + strand at 6655.491 kb on + strand at 6655.492 kb on - strand at 6655.551 kb on + strand, within Pf6N2E2_5838 at 6655.552 kb on - strand, within Pf6N2E2_5838 at 6655.552 kb on - strand, within Pf6N2E2_5838 at 6655.674 kb on + strand, within Pf6N2E2_5838 at 6655.674 kb on + strand, within Pf6N2E2_5838 at 6655.674 kb on + strand, within Pf6N2E2_5838 at 6655.675 kb on - strand, within Pf6N2E2_5838 at 6655.675 kb on - strand, within Pf6N2E2_5838 at 6655.675 kb on - strand, within Pf6N2E2_5838 at 6655.683 kb on + strand, within Pf6N2E2_5838 at 6655.684 kb on - strand, within Pf6N2E2_5838 at 6655.752 kb on + strand, within Pf6N2E2_5838 at 6655.752 kb on + strand, within Pf6N2E2_5838 at 6655.752 kb on + strand, within Pf6N2E2_5838 at 6655.753 kb on - strand, within Pf6N2E2_5838 at 6655.830 kb on + strand, within Pf6N2E2_5838 at 6655.830 kb on + strand, within Pf6N2E2_5838 at 6655.830 kb on + strand, within Pf6N2E2_5838 at 6656.094 kb on + strand, within Pf6N2E2_5838 at 6656.094 kb on + strand, within Pf6N2E2_5838 at 6656.094 kb on + strand, within Pf6N2E2_5838 at 6656.094 kb on + strand, within Pf6N2E2_5838 at 6656.094 kb on + strand, within Pf6N2E2_5838 at 6656.095 kb on - strand, within Pf6N2E2_5838 at 6656.095 kb on - strand, within Pf6N2E2_5838 at 6656.112 kb on + strand, within Pf6N2E2_5838 at 6656.112 kb on + strand, within Pf6N2E2_5838 at 6656.113 kb on - strand, within Pf6N2E2_5838 at 6656.113 kb on - strand, within Pf6N2E2_5838 at 6656.223 kb on + strand at 6656.223 kb on + strand at 6656.223 kb on + strand at 6656.223 kb on + strand at 6656.224 kb on - strand at 6656.224 kb on - strand at 6656.224 kb on - strand at 6656.224 kb on - strand at 6656.233 kb on + strand at 6656.233 kb on + strand at 6656.234 kb on - strand at 6656.234 kb on - strand at 6656.234 kb on - strand at 6656.359 kb on + strand at 6656.359 kb on + strand at 6656.380 kb on + strand at 6656.380 kb on + strand at 6656.380 kb on + strand at 6656.384 kb on - strand at 6656.480 kb on + strand at 6656.498 kb on + strand at 6656.689 kb on + strand, within Pf6N2E2_5839 at 6656.690 kb on - strand, within Pf6N2E2_5839 at 6656.734 kb on + strand, within Pf6N2E2_5839 at 6656.735 kb on - strand, within Pf6N2E2_5839 at 6656.761 kb on + strand, within Pf6N2E2_5839 at 6656.761 kb on + strand, within Pf6N2E2_5839 at 6656.761 kb on + strand, within Pf6N2E2_5839 at 6656.762 kb on - strand, within Pf6N2E2_5839 at 6656.762 kb on - strand, within Pf6N2E2_5839 at 6656.762 kb on - strand, within Pf6N2E2_5839 at 6656.818 kb on + strand, within Pf6N2E2_5839 at 6656.938 kb on + strand, within Pf6N2E2_5839 at 6656.939 kb on - strand, within Pf6N2E2_5839 at 6656.992 kb on + strand, within Pf6N2E2_5839 at 6656.993 kb on - strand, within Pf6N2E2_5839 at 6656.993 kb on - strand, within Pf6N2E2_5839 at 6656.993 kb on - strand, within Pf6N2E2_5839 at 6657.013 kb on - strand, within Pf6N2E2_5839 at 6657.031 kb on + strand, within Pf6N2E2_5839 at 6657.032 kb on - strand, within Pf6N2E2_5839 at 6657.032 kb on - strand, within Pf6N2E2_5839
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 6,654,481 + Pf6N2E2_5837 0.13 +1.0 6,655,272 + +1.0 6,655,272 + -0.0 6,655,272 + -0.0 6,655,272 + -2.9 6,655,273 - +1.0 6,655,273 - +1.0 6,655,491 + -6.2 6,655,491 + -1.3 6,655,492 - +1.0 6,655,551 + Pf6N2E2_5838 0.15 +1.0 6,655,552 - Pf6N2E2_5838 0.16 -0.0 6,655,552 - Pf6N2E2_5838 0.16 +1.0 6,655,674 + Pf6N2E2_5838 0.31 -0.6 6,655,674 + Pf6N2E2_5838 0.31 -0.0 6,655,674 + Pf6N2E2_5838 0.31 -1.3 6,655,675 - Pf6N2E2_5838 0.31 -0.0 6,655,675 - Pf6N2E2_5838 0.31 +1.0 6,655,675 - Pf6N2E2_5838 0.31 -0.6 6,655,683 + Pf6N2E2_5838 0.32 +1.0 6,655,684 - Pf6N2E2_5838 0.32 +1.0 6,655,752 + Pf6N2E2_5838 0.41 -0.0 6,655,752 + Pf6N2E2_5838 0.41 -2.3 6,655,752 + Pf6N2E2_5838 0.41 -0.0 6,655,753 - Pf6N2E2_5838 0.41 +1.0 6,655,830 + Pf6N2E2_5838 0.51 +1.0 6,655,830 + Pf6N2E2_5838 0.51 -0.6 6,655,830 + Pf6N2E2_5838 0.51 -0.0 6,656,094 + Pf6N2E2_5838 0.85 -6.6 6,656,094 + Pf6N2E2_5838 0.85 +1.0 6,656,094 + Pf6N2E2_5838 0.85 +1.0 6,656,094 + Pf6N2E2_5838 0.85 -0.0 6,656,094 + Pf6N2E2_5838 0.85 +1.0 6,656,095 - Pf6N2E2_5838 0.85 +1.0 6,656,095 - Pf6N2E2_5838 0.85 +1.0 6,656,112 + Pf6N2E2_5838 0.87 -1.0 6,656,112 + Pf6N2E2_5838 0.87 -0.6 6,656,113 - Pf6N2E2_5838 0.87 +1.0 6,656,113 - Pf6N2E2_5838 0.87 -0.0 6,656,223 + +1.0 6,656,223 + +1.0 6,656,223 + -0.0 6,656,223 + -3.9 6,656,224 - -0.0 6,656,224 - +0.6 6,656,224 - -0.6 6,656,224 - +1.0 6,656,233 + -0.0 6,656,233 + -0.6 6,656,234 - -0.6 6,656,234 - +1.0 6,656,234 - +1.0 6,656,359 + -0.6 6,656,359 + +1.0 6,656,380 + +1.0 6,656,380 + -2.7 6,656,380 + +1.0 6,656,384 - +1.0 6,656,480 + -0.0 6,656,498 + +1.0 6,656,689 + Pf6N2E2_5839 0.17 -0.0 6,656,690 - Pf6N2E2_5839 0.17 -1.5 6,656,734 + Pf6N2E2_5839 0.20 +1.0 6,656,735 - Pf6N2E2_5839 0.20 +1.0 6,656,761 + Pf6N2E2_5839 0.21 -0.6 6,656,761 + Pf6N2E2_5839 0.21 -0.6 6,656,761 + Pf6N2E2_5839 0.21 -0.0 6,656,762 - Pf6N2E2_5839 0.22 -0.0 6,656,762 - Pf6N2E2_5839 0.22 -1.2 6,656,762 - Pf6N2E2_5839 0.22 -2.3 6,656,818 + Pf6N2E2_5839 0.25 -0.6 6,656,938 + Pf6N2E2_5839 0.31 -0.0 6,656,939 - Pf6N2E2_5839 0.32 -1.8 6,656,992 + Pf6N2E2_5839 0.35 -1.3 6,656,993 - Pf6N2E2_5839 0.35 +1.0 6,656,993 - Pf6N2E2_5839 0.35 -1.1 6,656,993 - Pf6N2E2_5839 0.35 +1.0 6,657,013 - Pf6N2E2_5839 0.36 -1.5 6,657,031 + Pf6N2E2_5839 0.37 -1.0 6,657,032 - Pf6N2E2_5839 0.37 -1.4 6,657,032 - Pf6N2E2_5839 0.37 -0.0
Or see this region's nucleotide sequence