Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5803 and Pf6N2E2_5804 are separated by 54 nucleotides Pf6N2E2_5804 and Pf6N2E2_5805 overlap by 4 nucleotides Pf6N2E2_5805 and Pf6N2E2_5806 are separated by 45 nucleotides Pf6N2E2_5806 and Pf6N2E2_5807 are separated by 191 nucleotides Pf6N2E2_5807 and Pf6N2E2_5808 are separated by 96 nucleotides
Pf6N2E2_5803 - Allantoicase (EC 3.5.3.4), at 6,620,661 to 6,621,656
_5803
Pf6N2E2_5804 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 6,621,711 to 6,622,226
_5804
Pf6N2E2_5805 - Uricase (urate oxidase) (EC 1.7.3.3), at 6,622,223 to 6,623,164
_5805
Pf6N2E2_5806 - hypothetical protein, at 6,623,210 to 6,623,386
_5806
Pf6N2E2_5807 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 6,623,578 to 6,623,931
_5807
Pf6N2E2_5808 - hypothetical protein, at 6,624,028 to 6,625,617
_5808
Position (kb)
6622
6623
6624 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 6621.367 kb on + strand, within Pf6N2E2_5803 at 6621.368 kb on - strand, within Pf6N2E2_5803 at 6621.368 kb on - strand, within Pf6N2E2_5803 at 6621.371 kb on + strand, within Pf6N2E2_5803 at 6621.372 kb on - strand, within Pf6N2E2_5803 at 6621.639 kb on + strand at 6621.646 kb on + strand at 6621.646 kb on + strand at 6621.647 kb on - strand at 6621.647 kb on - strand at 6621.647 kb on - strand at 6621.673 kb on + strand at 6621.673 kb on + strand at 6621.673 kb on + strand at 6621.674 kb on - strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.676 kb on + strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.677 kb on - strand at 6621.840 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.877 kb on + strand, within Pf6N2E2_5804 at 6621.878 kb on - strand, within Pf6N2E2_5804 at 6622.153 kb on + strand, within Pf6N2E2_5804 at 6622.154 kb on - strand, within Pf6N2E2_5804 at 6622.257 kb on + strand at 6622.288 kb on + strand at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.333 kb on - strand, within Pf6N2E2_5805 at 6622.435 kb on - strand, within Pf6N2E2_5805 at 6622.496 kb on + strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.522 kb on + strand, within Pf6N2E2_5805 at 6622.582 kb on - strand, within Pf6N2E2_5805 at 6622.599 kb on + strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.674 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.219 kb on - strand at 6623.272 kb on - strand, within Pf6N2E2_5806 at 6623.316 kb on - strand, within Pf6N2E2_5806 at 6623.316 kb on - strand, within Pf6N2E2_5806 at 6623.348 kb on - strand, within Pf6N2E2_5806 at 6623.367 kb on + strand, within Pf6N2E2_5806 at 6623.384 kb on + strand at 6623.428 kb on - strand at 6623.592 kb on + strand at 6623.592 kb on + strand at 6623.593 kb on - strand at 6623.650 kb on + strand, within Pf6N2E2_5807 at 6623.740 kb on - strand, within Pf6N2E2_5807 at 6623.743 kb on + strand, within Pf6N2E2_5807 at 6623.743 kb on + strand, within Pf6N2E2_5807 at 6623.743 kb on + strand, within Pf6N2E2_5807 at 6623.744 kb on - strand, within Pf6N2E2_5807 at 6623.759 kb on - strand, within Pf6N2E2_5807 at 6623.786 kb on - strand, within Pf6N2E2_5807 at 6623.875 kb on + strand, within Pf6N2E2_5807 at 6623.876 kb on - strand, within Pf6N2E2_5807 at 6623.902 kb on + strand at 6623.903 kb on - strand at 6623.909 kb on - strand at 6623.917 kb on + strand at 6623.917 kb on + strand at 6623.918 kb on - strand at 6623.918 kb on - strand at 6623.918 kb on - strand at 6623.918 kb on - strand at 6624.025 kb on + strand at 6624.029 kb on + strand at 6624.030 kb on - strand at 6624.030 kb on - strand at 6624.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 6,621,367 + Pf6N2E2_5803 0.71 -0.0 6,621,368 - Pf6N2E2_5803 0.71 -1.3 6,621,368 - Pf6N2E2_5803 0.71 -0.0 6,621,371 + Pf6N2E2_5803 0.71 +1.0 6,621,372 - Pf6N2E2_5803 0.71 +1.0 6,621,639 + +1.0 6,621,646 + -3.2 6,621,646 + -4.6 6,621,647 - +1.0 6,621,647 - -1.0 6,621,647 - +1.0 6,621,673 + +1.0 6,621,673 + -0.0 6,621,673 + +1.0 6,621,674 - -0.0 6,621,676 + -3.2 6,621,676 + -0.6 6,621,676 + +1.0 6,621,676 + +1.0 6,621,677 - +1.0 6,621,677 - -1.3 6,621,677 - +1.0 6,621,677 - -0.0 6,621,677 - -0.5 6,621,840 + Pf6N2E2_5804 0.25 +1.0 6,621,877 + Pf6N2E2_5804 0.32 +1.0 6,621,877 + Pf6N2E2_5804 0.32 -0.0 6,621,877 + Pf6N2E2_5804 0.32 -1.8 6,621,878 - Pf6N2E2_5804 0.32 +1.0 6,622,153 + Pf6N2E2_5804 0.86 -0.0 6,622,154 - Pf6N2E2_5804 0.86 +1.0 6,622,257 + -0.6 6,622,288 + +1.0 6,622,332 + Pf6N2E2_5805 0.12 +1.0 6,622,332 + Pf6N2E2_5805 0.12 -0.0 6,622,333 - Pf6N2E2_5805 0.12 -0.6 6,622,435 - Pf6N2E2_5805 0.23 +1.0 6,622,496 + Pf6N2E2_5805 0.29 -2.0 6,622,497 - Pf6N2E2_5805 0.29 +1.0 6,622,497 - Pf6N2E2_5805 0.29 +1.0 6,622,522 + Pf6N2E2_5805 0.32 +1.0 6,622,582 - Pf6N2E2_5805 0.38 -3.5 6,622,599 + Pf6N2E2_5805 0.40 +1.0 6,622,600 - Pf6N2E2_5805 0.40 +1.0 6,622,600 - Pf6N2E2_5805 0.40 -0.0 6,622,600 - Pf6N2E2_5805 0.40 -0.0 6,622,600 - Pf6N2E2_5805 0.40 +1.0 6,622,615 - Pf6N2E2_5805 0.42 -5.6 6,622,615 - Pf6N2E2_5805 0.42 -0.0 6,622,674 + Pf6N2E2_5805 0.48 -2.0 6,622,761 + Pf6N2E2_5805 0.57 +1.0 6,622,761 + Pf6N2E2_5805 0.57 +1.0 6,622,761 + Pf6N2E2_5805 0.57 -0.0 6,622,761 + Pf6N2E2_5805 0.57 +1.0 6,622,761 + Pf6N2E2_5805 0.57 +1.0 6,622,761 + Pf6N2E2_5805 0.57 -0.0 6,622,761 + Pf6N2E2_5805 0.57 -0.0 6,622,761 + Pf6N2E2_5805 0.57 +1.0 6,622,761 + Pf6N2E2_5805 0.57 +1.0 6,622,762 - Pf6N2E2_5805 0.57 -0.6 6,622,762 - Pf6N2E2_5805 0.57 -0.0 6,622,762 - Pf6N2E2_5805 0.57 +1.0 6,622,762 - Pf6N2E2_5805 0.57 +1.0 6,622,762 - Pf6N2E2_5805 0.57 +1.0 6,623,218 + +1.0 6,623,218 + -2.7 6,623,218 + +1.0 6,623,219 - +1.0 6,623,272 - Pf6N2E2_5806 0.35 -0.0 6,623,316 - Pf6N2E2_5806 0.60 +1.0 6,623,316 - Pf6N2E2_5806 0.60 -0.0 6,623,348 - Pf6N2E2_5806 0.78 -1.3 6,623,367 + Pf6N2E2_5806 0.89 -0.0 6,623,384 + -0.0 6,623,428 - -0.0 6,623,592 + -0.6 6,623,592 + +1.0 6,623,593 - +1.0 6,623,650 + Pf6N2E2_5807 0.20 -0.0 6,623,740 - Pf6N2E2_5807 0.46 -1.8 6,623,743 + Pf6N2E2_5807 0.47 -0.0 6,623,743 + Pf6N2E2_5807 0.47 +1.0 6,623,743 + Pf6N2E2_5807 0.47 -1.0 6,623,744 - Pf6N2E2_5807 0.47 -0.0 6,623,759 - Pf6N2E2_5807 0.51 +1.0 6,623,786 - Pf6N2E2_5807 0.59 -0.6 6,623,875 + Pf6N2E2_5807 0.84 -1.8 6,623,876 - Pf6N2E2_5807 0.84 -0.6 6,623,902 + -1.3 6,623,903 - +1.0 6,623,909 - +1.0 6,623,917 + +1.0 6,623,917 + +1.0 6,623,918 - -5.4 6,623,918 - +1.0 6,623,918 - -6.3 6,623,918 - -0.0 6,624,025 + +0.4 6,624,029 + -0.5 6,624,030 - -0.0 6,624,030 - +0.1 6,624,030 - -1.1
Or see this region's nucleotide sequence