Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5344

Experiment: Ying_Nucleobase14 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5342 and Pf6N2E2_5343 overlap by 4 nucleotidesPf6N2E2_5343 and Pf6N2E2_5344 are separated by 102 nucleotidesPf6N2E2_5344 and Pf6N2E2_5345 overlap by 1 nucleotides Pf6N2E2_5342 - 21 kDa hemolysin precursor, at 6,136,430 to 6,137,008 _5342 Pf6N2E2_5343 - Phosphoheptose isomerase (EC 5.3.1.-), at 6,137,005 to 6,137,598 _5343 Pf6N2E2_5344 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 6,137,701 to 6,138,063 _5344 Pf6N2E2_5345 - LppC putative lipoprotein, at 6,138,063 to 6,139,874 _5345 Position (kb) 6137 6138 6139Strain fitness (log2 ratio) -2 -1 0 1at 6136.704 kb on - strand, within Pf6N2E2_5342at 6136.704 kb on - strand, within Pf6N2E2_5342at 6136.704 kb on - strand, within Pf6N2E2_5342at 6137.611 kb on - strandat 6137.611 kb on - strandat 6137.611 kb on - strandat 6137.611 kb on - strandat 6137.611 kb on - strandat 6137.611 kb on - strandat 6137.615 kb on - strandat 6137.814 kb on - strand, within Pf6N2E2_5344at 6137.884 kb on - strand, within Pf6N2E2_5344at 6137.912 kb on - strand, within Pf6N2E2_5344at 6137.912 kb on - strand, within Pf6N2E2_5344at 6137.915 kb on - strand, within Pf6N2E2_5344at 6137.915 kb on - strand, within Pf6N2E2_5344at 6137.915 kb on - strand, within Pf6N2E2_5344at 6137.915 kb on - strand, within Pf6N2E2_5344at 6137.915 kb on - strand, within Pf6N2E2_5344at 6137.915 kb on - strand, within Pf6N2E2_5344at 6137.920 kb on - strand, within Pf6N2E2_5344at 6137.920 kb on - strand, within Pf6N2E2_5344at 6138.132 kb on + strandat 6138.237 kb on + strandat 6138.237 kb on + strandat 6138.250 kb on + strand, within Pf6N2E2_5345at 6138.250 kb on + strand, within Pf6N2E2_5345at 6138.370 kb on + strand, within Pf6N2E2_5345at 6138.371 kb on - strand, within Pf6N2E2_5345at 6138.416 kb on - strand, within Pf6N2E2_5345at 6138.436 kb on + strand, within Pf6N2E2_5345at 6138.437 kb on - strand, within Pf6N2E2_5345at 6138.847 kb on + strand, within Pf6N2E2_5345at 6138.847 kb on + strand, within Pf6N2E2_5345at 6138.847 kb on + strand, within Pf6N2E2_5345at 6138.848 kb on - strand, within Pf6N2E2_5345at 6138.848 kb on - strand, within Pf6N2E2_5345at 6138.848 kb on - strand, within Pf6N2E2_5345at 6138.996 kb on + strand, within Pf6N2E2_5345at 6138.997 kb on + strand, within Pf6N2E2_5345at 6138.997 kb on + strand, within Pf6N2E2_5345at 6138.998 kb on - strand, within Pf6N2E2_5345at 6139.043 kb on - strand, within Pf6N2E2_5345at 6139.043 kb on - strand, within Pf6N2E2_5345

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2
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6,136,704 - Pf6N2E2_5342 0.47 +1.0
6,136,704 - Pf6N2E2_5342 0.47 +1.0
6,136,704 - Pf6N2E2_5342 0.47 +0.0
6,137,611 - +0.1
6,137,611 - +1.0
6,137,611 - +1.0
6,137,611 - -1.8
6,137,611 - +0.0
6,137,611 - +1.0
6,137,615 - +1.0
6,137,814 - Pf6N2E2_5344 0.31 -1.0
6,137,884 - Pf6N2E2_5344 0.50 +0.0
6,137,912 - Pf6N2E2_5344 0.58 +0.1
6,137,912 - Pf6N2E2_5344 0.58 -1.0
6,137,915 - Pf6N2E2_5344 0.59 +0.0
6,137,915 - Pf6N2E2_5344 0.59 +1.0
6,137,915 - Pf6N2E2_5344 0.59 -1.0
6,137,915 - Pf6N2E2_5344 0.59 -0.4
6,137,915 - Pf6N2E2_5344 0.59 -0.2
6,137,915 - Pf6N2E2_5344 0.59 +0.4
6,137,920 - Pf6N2E2_5344 0.60 +1.0
6,137,920 - Pf6N2E2_5344 0.60 +0.0
6,138,132 + -1.6
6,138,237 + -0.6
6,138,237 + +1.0
6,138,250 + Pf6N2E2_5345 0.10 -2.3
6,138,250 + Pf6N2E2_5345 0.10 +0.0
6,138,370 + Pf6N2E2_5345 0.17 -2.0
6,138,371 - Pf6N2E2_5345 0.17 -0.6
6,138,416 - Pf6N2E2_5345 0.19 -1.0
6,138,436 + Pf6N2E2_5345 0.21 -2.2
6,138,437 - Pf6N2E2_5345 0.21 +1.0
6,138,847 + Pf6N2E2_5345 0.43 +1.0
6,138,847 + Pf6N2E2_5345 0.43 +1.0
6,138,847 + Pf6N2E2_5345 0.43 +0.0
6,138,848 - Pf6N2E2_5345 0.43 -0.6
6,138,848 - Pf6N2E2_5345 0.43 +1.0
6,138,848 - Pf6N2E2_5345 0.43 -1.2
6,138,996 + Pf6N2E2_5345 0.51 -0.6
6,138,997 + Pf6N2E2_5345 0.52 +1.0
6,138,997 + Pf6N2E2_5345 0.52 +0.0
6,138,998 - Pf6N2E2_5345 0.52 -0.6
6,139,043 - Pf6N2E2_5345 0.54 +0.0
6,139,043 - Pf6N2E2_5345 0.54 +0.0

Or see this region's nucleotide sequence