Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5266 and Pf6N2E2_5267 are separated by 40 nucleotides Pf6N2E2_5267 and Pf6N2E2_5268 are separated by 310 nucleotides
Pf6N2E2_5266 - Glycosyltransferase, at 6,060,149 to 6,061,348
_5266
Pf6N2E2_5267 - Sulfate transporter, CysZ-type, at 6,061,389 to 6,062,144
_5267
Pf6N2E2_5268 - Thioredoxin reductase (EC 1.8.1.9), at 6,062,455 to 6,063,417
_5268
Position (kb)
6061
6062
6063 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.405 kb on + strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.406 kb on - strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.501 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.894 kb on + strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.895 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.906 kb on - strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.942 kb on + strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.943 kb on - strand, within Pf6N2E2_5266 at 6060.982 kb on + strand, within Pf6N2E2_5266 at 6061.041 kb on + strand, within Pf6N2E2_5266 at 6061.041 kb on + strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.042 kb on - strand, within Pf6N2E2_5266 at 6061.199 kb on + strand, within Pf6N2E2_5266 at 6061.303 kb on + strand at 6061.304 kb on - strand at 6061.609 kb on + strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.610 kb on - strand, within Pf6N2E2_5267 at 6061.720 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.936 kb on + strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6061.937 kb on - strand, within Pf6N2E2_5267 at 6062.014 kb on + strand, within Pf6N2E2_5267 at 6062.015 kb on - strand, within Pf6N2E2_5267 at 6062.015 kb on - strand, within Pf6N2E2_5267 at 6062.061 kb on + strand, within Pf6N2E2_5267 at 6062.061 kb on + strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.062 kb on - strand, within Pf6N2E2_5267 at 6062.114 kb on - strand at 6062.144 kb on + strand at 6062.144 kb on + strand at 6062.144 kb on + strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.145 kb on - strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.220 kb on + strand at 6062.221 kb on - strand at 6062.221 kb on - strand at 6062.249 kb on - strand at 6062.269 kb on + strand at 6062.280 kb on + strand at 6062.295 kb on + strand at 6062.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 6,060,405 + Pf6N2E2_5266 0.21 +1.0 6,060,405 + Pf6N2E2_5266 0.21 +1.0 6,060,406 - Pf6N2E2_5266 0.21 -0.7 6,060,406 - Pf6N2E2_5266 0.21 +0.0 6,060,501 + Pf6N2E2_5266 0.29 -0.9 6,060,501 + Pf6N2E2_5266 0.29 +1.0 6,060,894 + Pf6N2E2_5266 0.62 -0.2 6,060,894 + Pf6N2E2_5266 0.62 -1.0 6,060,895 - Pf6N2E2_5266 0.62 +2.0 6,060,895 - Pf6N2E2_5266 0.62 -0.2 6,060,895 - Pf6N2E2_5266 0.62 +0.0 6,060,906 - Pf6N2E2_5266 0.63 +1.0 6,060,906 - Pf6N2E2_5266 0.63 +1.0 6,060,942 + Pf6N2E2_5266 0.66 -0.4 6,060,942 + Pf6N2E2_5266 0.66 +1.0 6,060,943 - Pf6N2E2_5266 0.66 +1.0 6,060,943 - Pf6N2E2_5266 0.66 -0.6 6,060,943 - Pf6N2E2_5266 0.66 +1.0 6,060,982 + Pf6N2E2_5266 0.69 +0.0 6,061,041 + Pf6N2E2_5266 0.74 +1.0 6,061,041 + Pf6N2E2_5266 0.74 +1.0 6,061,042 - Pf6N2E2_5266 0.74 +0.4 6,061,042 - Pf6N2E2_5266 0.74 +1.0 6,061,042 - Pf6N2E2_5266 0.74 +1.0 6,061,199 + Pf6N2E2_5266 0.88 +0.0 6,061,303 + -1.1 6,061,304 - +1.0 6,061,609 + Pf6N2E2_5267 0.29 +0.0 6,061,610 - Pf6N2E2_5267 0.29 +1.0 6,061,610 - Pf6N2E2_5267 0.29 -0.6 6,061,610 - Pf6N2E2_5267 0.29 -2.4 6,061,610 - Pf6N2E2_5267 0.29 +0.0 6,061,720 + Pf6N2E2_5267 0.44 +1.0 6,061,936 + Pf6N2E2_5267 0.72 +0.0 6,061,936 + Pf6N2E2_5267 0.72 -2.8 6,061,936 + Pf6N2E2_5267 0.72 -0.7 6,061,936 + Pf6N2E2_5267 0.72 +0.0 6,061,936 + Pf6N2E2_5267 0.72 -0.3 6,061,936 + Pf6N2E2_5267 0.72 +0.0 6,061,936 + Pf6N2E2_5267 0.72 +1.0 6,061,936 + Pf6N2E2_5267 0.72 -1.0 6,061,936 + Pf6N2E2_5267 0.72 +1.0 6,061,937 - Pf6N2E2_5267 0.72 +0.0 6,061,937 - Pf6N2E2_5267 0.72 +1.0 6,061,937 - Pf6N2E2_5267 0.72 -0.9 6,061,937 - Pf6N2E2_5267 0.72 +0.0 6,061,937 - Pf6N2E2_5267 0.72 -1.0 6,061,937 - Pf6N2E2_5267 0.72 +1.0 6,061,937 - Pf6N2E2_5267 0.72 +1.0 6,061,937 - Pf6N2E2_5267 0.72 -0.6 6,061,937 - Pf6N2E2_5267 0.72 +0.0 6,062,014 + Pf6N2E2_5267 0.83 +0.0 6,062,015 - Pf6N2E2_5267 0.83 +1.0 6,062,015 - Pf6N2E2_5267 0.83 +1.0 6,062,061 + Pf6N2E2_5267 0.89 +1.0 6,062,061 + Pf6N2E2_5267 0.89 -0.6 6,062,062 - Pf6N2E2_5267 0.89 +0.0 6,062,062 - Pf6N2E2_5267 0.89 +1.0 6,062,062 - Pf6N2E2_5267 0.89 -1.0 6,062,114 - +1.0 6,062,144 + +0.0 6,062,144 + +0.0 6,062,144 + +1.0 6,062,145 - -0.6 6,062,145 - -2.0 6,062,145 - +1.0 6,062,145 - -1.0 6,062,145 - +1.0 6,062,145 - +0.3 6,062,145 - +0.0 6,062,145 - +1.0 6,062,145 - -1.3 6,062,145 - +1.0 6,062,220 + +1.0 6,062,220 + +1.0 6,062,220 + -0.6 6,062,220 + -1.3 6,062,220 + -0.9 6,062,220 + +1.0 6,062,220 + +0.0 6,062,221 - -0.6 6,062,221 - -0.8 6,062,249 - +0.4 6,062,269 + -1.7 6,062,280 + -0.6 6,062,295 + +1.0 6,062,295 + -2.0
Or see this region's nucleotide sequence