Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4968 and Pf6N2E2_4969 overlap by 8 nucleotides Pf6N2E2_4969 and Pf6N2E2_4970 are separated by 443 nucleotides
Pf6N2E2_4968 - DNA cytosine methyltransferase M.NgoMIII, at 5,731,516 to 5,732,685
_4968
Pf6N2E2_4969 - hypothetical protein, at 5,732,678 to 5,732,830
_4969
Pf6N2E2_4970 - Serine phosphatase RsbU, regulator of sigma subunit, at 5,733,274 to 5,735,517
_4970
Position (kb)
5732
5733 Strain fitness (log2 ratio)
-1
0
1 at 5732.751 kb on - strand, within Pf6N2E2_4969 at 5732.859 kb on - strand at 5732.859 kb on - strand at 5732.883 kb on - strand at 5732.883 kb on - strand at 5732.885 kb on - strand at 5732.906 kb on - strand at 5732.906 kb on - strand at 5732.948 kb on - strand at 5732.983 kb on - strand at 5732.983 kb on - strand at 5732.998 kb on - strand at 5732.998 kb on - strand at 5733.003 kb on - strand at 5733.030 kb on + strand at 5733.030 kb on + strand at 5733.031 kb on - strand at 5733.031 kb on - strand at 5733.052 kb on - strand at 5733.097 kb on + strand at 5733.097 kb on + strand at 5733.097 kb on + strand at 5733.097 kb on + strand at 5733.098 kb on - strand at 5733.098 kb on - strand at 5733.098 kb on - strand at 5733.101 kb on + strand at 5733.101 kb on + strand at 5733.101 kb on + strand at 5733.101 kb on + strand at 5733.101 kb on + strand at 5733.101 kb on + strand at 5733.102 kb on - strand at 5733.102 kb on - strand at 5733.102 kb on - strand at 5733.129 kb on - strand at 5733.184 kb on + strand at 5733.184 kb on + strand at 5733.185 kb on - strand at 5733.185 kb on - strand at 5733.185 kb on - strand at 5733.204 kb on + strand at 5733.208 kb on + strand at 5733.208 kb on + strand at 5733.208 kb on + strand at 5733.208 kb on + strand at 5733.208 kb on + strand at 5733.208 kb on + strand at 5733.209 kb on - strand at 5733.209 kb on - strand at 5733.209 kb on - strand at 5733.209 kb on - strand at 5733.257 kb on + strand at 5733.258 kb on - strand at 5733.338 kb on + strand at 5733.339 kb on - strand at 5733.365 kb on + strand at 5733.365 kb on + strand at 5733.365 kb on + strand at 5733.366 kb on - strand at 5733.366 kb on - strand at 5733.366 kb on - strand at 5733.369 kb on + strand at 5733.369 kb on + strand at 5733.369 kb on + strand at 5733.369 kb on + strand at 5733.369 kb on + strand at 5733.369 kb on + strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.370 kb on - strand at 5733.420 kb on - strand at 5733.420 kb on - strand at 5733.452 kb on + strand at 5733.452 kb on + strand at 5733.453 kb on - strand at 5733.560 kb on - strand, within Pf6N2E2_4970 at 5733.591 kb on + strand, within Pf6N2E2_4970 at 5733.648 kb on - strand, within Pf6N2E2_4970 at 5733.648 kb on - strand, within Pf6N2E2_4970 at 5733.648 kb on - strand, within Pf6N2E2_4970 at 5733.676 kb on - strand, within Pf6N2E2_4970 at 5733.678 kb on + strand, within Pf6N2E2_4970 at 5733.678 kb on + strand, within Pf6N2E2_4970 at 5733.679 kb on - strand, within Pf6N2E2_4970
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 5,732,751 - Pf6N2E2_4969 0.48 -1.2 5,732,859 - -1.1 5,732,859 - -1.5 5,732,883 - -0.4 5,732,883 - +0.5 5,732,885 - +1.1 5,732,906 - -0.3 5,732,906 - +0.1 5,732,948 - -0.4 5,732,983 - +0.1 5,732,983 - -0.9 5,732,998 - +0.1 5,732,998 - -1.5 5,733,003 - +0.1 5,733,030 + +1.1 5,733,030 + -1.2 5,733,031 - +1.1 5,733,031 - +0.1 5,733,052 - -0.5 5,733,097 + -0.5 5,733,097 + -0.5 5,733,097 + +0.4 5,733,097 + -0.9 5,733,098 - +1.1 5,733,098 - -0.9 5,733,098 - -0.9 5,733,101 + +1.1 5,733,101 + +0.1 5,733,101 + +1.1 5,733,101 + +1.1 5,733,101 + -0.6 5,733,101 + +1.1 5,733,102 - +0.1 5,733,102 - +1.1 5,733,102 - +1.1 5,733,129 - +1.1 5,733,184 + +1.1 5,733,184 + +1.1 5,733,185 - -1.6 5,733,185 - -1.2 5,733,185 - +1.1 5,733,204 + +0.1 5,733,208 + +1.1 5,733,208 + +1.1 5,733,208 + +0.1 5,733,208 + -1.2 5,733,208 + -0.9 5,733,208 + +0.1 5,733,209 - +1.1 5,733,209 - -0.9 5,733,209 - +1.1 5,733,209 - +0.1 5,733,257 + +0.1 5,733,258 - -0.8 5,733,338 + +1.1 5,733,339 - -0.0 5,733,365 + +0.7 5,733,365 + +1.1 5,733,365 + +1.1 5,733,366 - +0.1 5,733,366 - +1.1 5,733,366 - +1.1 5,733,369 + +1.1 5,733,369 + -0.5 5,733,369 + +1.1 5,733,369 + +1.1 5,733,369 + +0.1 5,733,369 + -0.1 5,733,370 - +0.1 5,733,370 - -0.9 5,733,370 - +0.1 5,733,370 - +0.1 5,733,370 - -0.3 5,733,370 - +0.1 5,733,370 - -0.5 5,733,370 - +0.4 5,733,370 - -0.5 5,733,370 - +1.1 5,733,420 - -0.5 5,733,420 - +0.1 5,733,452 + +0.1 5,733,452 + +1.1 5,733,453 - +1.1 5,733,560 - Pf6N2E2_4970 0.13 -0.1 5,733,591 + Pf6N2E2_4970 0.14 +0.1 5,733,648 - Pf6N2E2_4970 0.17 +1.1 5,733,648 - Pf6N2E2_4970 0.17 +1.1 5,733,648 - Pf6N2E2_4970 0.17 +1.1 5,733,676 - Pf6N2E2_4970 0.18 +1.1 5,733,678 + Pf6N2E2_4970 0.18 -0.5 5,733,678 + Pf6N2E2_4970 0.18 +1.1 5,733,679 - Pf6N2E2_4970 0.18 +1.1
Or see this region's nucleotide sequence